RESUMEN
We studied phagocytic activity of macrophages towards hybrid molecular nanosomal compositions consisting of 150-800-nm nanoliposomes containing oxidized dextrans with a molecular weight of 35 and 60 kDa obtained by chemical ("permanganate") and radiochemical oxidation of dextran conjugated with isonicotinic acid hydrazide (dextrazides, intracellular prolonged antituberculous drugs). Phagocytic activity of macrophages towards hybrid molecular nanosomal compositions containing dextrazides obtained by chemical oxidation of dextrans is higher than activity towards hybrid molecular nanosomal compositions containing dextrazides prepared by radiochemical oxidation and depends on the size of hybrid molecular nanosomal compositions and molecular weight of oxidized dextrans.
Asunto(s)
Dextranos/química , Isoniazida/química , Liposomas/metabolismo , Macrófagos Peritoneales/metabolismo , Nanocompuestos/química , Fagocitosis/fisiología , Animales , Células Cultivadas , Liposomas/química , Masculino , Ratones , Ratones Endogámicos BALB C , Oxidación-ReducciónRESUMEN
We studied phagocytic activity of macrophages against molecular-liposome hybrid compositions consisting of liposomes (diameter 200-450 nm) containing oxidized dextrans with a molecular weight of 35 or 60 kDa conjugated with the basic antituberculosis preparation isonicotinic acid hydrazide (dextrazides) during modeling of various disturbances of endocytosis function of phagocytic cells in vitro. Preincubation of macrophages with trypsin, colchicine, or sodium azide did not change the parameters of adhesion of molecular-liposome hybrid compositions to macrophages. It was found that preincubation of cells with colchicine or sodium azide reduced parameters of phagocytosis of the molecular-liposome hybrid compositions; this reduction did not depend on the molecular weight of dextrans entering the composition of the molecular-liposome hybrid compositions.
Asunto(s)
Dextranos/química , Isoniazida/química , Liposomas , Macrófagos/fisiología , Modelos Biológicos , Fagocitosis/fisiología , Animales , Portadores de Fármacos/química , Portadores de Fármacos/metabolismo , Inhibidores de la Síntesis de Ácidos Grasos/química , Liposomas/química , Liposomas/metabolismo , Macrófagos/citología , Masculino , Ratones , Ratones Endogámicos BALB CRESUMEN
The effects of molecular liposomal hybrid compositions consisting of liposomes (200-450 nm) containing oxidized dextrans (dextranals; 35-60 kDa) conjugated with isonicotinic acid hydrazide (dextrazides), their components, and native dextrans on the production of granulocytic macrophage CSF by peritoneal macrophages were studied in vitro. Dextranals proved to be more potent inductors of granulocytic macrophage CSF than native dextrans. Conjugation of nicotinic acid hydrazide with dextranals did not modify their capacity to stimulate the production of granulocytic macrophage CSF. Liposomes in the molecular liposomal hybrid compositions did not attenuate the dextrazide capacity to stimulate the production of granulocytic macrophage CSF. Molecular liposomal compositions containing 60 kDa dextrazide exhibited the most potent stimulatory effect on macrophage production of granulocytic macrophage CSF.
Asunto(s)
Dextranos , Inhibidores de la Síntesis de Ácidos Grasos , Factor Estimulante de Colonias de Granulocitos y Macrófagos/biosíntesis , Isoniazida , Liposomas , Macrófagos/metabolismo , Animales , Dextranos/química , Dextranos/metabolismo , Inhibidores de la Síntesis de Ácidos Grasos/química , Inhibidores de la Síntesis de Ácidos Grasos/metabolismo , Isoniazida/química , Isoniazida/metabolismo , Liposomas/química , Liposomas/metabolismo , Macrófagos/citología , Ratones , Ratones Endogámicos BALB CRESUMEN
We studied the dependence of in vitro dextran biocompatibility on the method of oxidation of 35-kDa dextran. The biocompatibility of dextran oxidized with potassium permanganate was higher compared to that obtained by radiochemical oxidation. It was related to the formation of peroxide compounds during radiochemical oxidation.
Asunto(s)
Anticoagulantes/farmacología , Dextranos/química , Dextranos/farmacología , Peritoneo/citología , Animales , Anticoagulantes/química , Adhesión Celular/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Células Cultivadas , Masculino , Ratones , Ratones Endogámicos BALB C , Oxidación-Reducción , Permanganato de Potasio/química , RadioquímicaRESUMEN
We studied the in vitro effect of hybrid molecular-nanosomal biocompatible compositions on cultured peritoneal cells. The compositions consisted of oxidized dextrans with a mean molecular weight of 35 and 60 kDa, which were obtained by chemical and radiochemical oxidation of dextran. Hybrid nanoliposomal compositions of chemically oxidized dextran (permanganate method) had greater biocompatibility and tropic activity to macrophages compared to nanoliposomes of radiochemically oxidized dextran.