RESUMEN
We performed transcriptome analysis of some human induced pluripotent stem cells, embryonic stem cells, and human somatic cells using DNA microarrays. PluriTest bioinformatic system was used for evaluation of cell pluripotency. Changes in the genome structure and status of X-chromosome gene expression was analyzed using microarray technology.
Asunto(s)
Células Madre Embrionarias/fisiología , Genes Ligados a X , Células Madre Pluripotentes Inducidas/fisiología , Transcriptoma , Células Cultivadas , ADN/genética , Células Madre Embrionarias/citología , Expresión Génica , Perfilación de la Expresión Génica , Humanos , Células Madre Pluripotentes Inducidas/citología , Análisis de Secuencia por Matrices de OligonucleótidosRESUMEN
The placebo-controlled trial of effectiveness of early intensive UHF therapy in 107 females at reproductive age with acute salpin-goophoritis has demonstrated high clinical and local immunomodulating effects of intensive UHF therapy (460 MHz, 3 procedures a day) in combination with adequate antibacterial treatment used as early as hospitalization day 1.
Asunto(s)
Ooforitis/diagnóstico por imagen , Modalidades de Fisioterapia/métodos , Terapia por Radiofrecuencia , Salpingitis/radioterapia , Enfermedad Aguda , Moco del Cuello Uterino/metabolismo , Femenino , Humanos , Interleucina-6/biosíntesis , Ooforitis/complicaciones , Ooforitis/diagnóstico , Radiografía , Salpingitis/complicaciones , Salpingitis/diagnósticoRESUMEN
The possibility of detecting cholera toxin genes in V.cholerae enterotoxigenic strains by the method of "nested" polymerase chain reaction with the use of primers on the DNA area of operon ctx of AB genes. The possibility of the detection of several V.cholerae cells by this method was shown with the use of a series of bacterial lysate dilutions. The newly developed test system for the detection of cholera toxin gene on the basis of the analysis of bacterial lysates of V.cholerae nontoxigenic strains, as well as Escherichia coli toxigenic and nontoxigenic strains, was shown to be highly specific.
Asunto(s)
Toxina del Cólera/genética , ADN Bacteriano/genética , Genes Bacterianos , Reacción en Cadena de la Polimerasa/métodos , Vibrio cholerae/genética , Secuencia de Bases , Cartilla de ADN , ADN Bacteriano/aislamiento & purificación , Electroforesis en Gel de Agar , Escherichia coli/genética , Datos de Secuencia Molecular , Operón/genética , Sensibilidad y EspecificidadRESUMEN
The method for the analysis of cholera toxin gene in V. cholerae strains was developed on the basis of polymerase chain reaction (PCR). This specific and highly sensitive method using primers affecting the site of the DNA of the operon of cholera toxin gene made it possible to identify one copy of V. cholerae genome. For the first time the content of cholera toxin gene in 4 V. cholerae (eltor) strains, obtained from the clinical material of cholera patients in Tajikistan and Dagestan, was shown with the use of PCR.