RESUMEN
Neuropeptide Y (NPY) is widely distributed in the autonomic nervous system and acts as a neurotransmitter and a trophic factor. However, there is no report concerning the expression of NPY and its receptors in the intestine during postnatal ontogenesis. In the current study, immunohistochemistry and western blot analysis was used to label NPY, Y1R, Y2R and Y5R receptors in the duodenum from rats of different ages (1-, 10-, 20-, 30-, 60-day-old and 2-year-old). The obtained data suggest age-dependent changes of NPY-mediated gut innervation. NPY-immunoreactive (IR) neurons were observed in the myenteric (MP) and submucous (SP) plexus from the moment of birth. In the MP, the percentage of NPY-IR neurons was low and varied from 4.1⯱â¯0.32 in 1-day-old to 2.9⯱â¯0.62 in 2-year-old rats. The proportion of NPY-IR myenteric neurons did not change significantly through the senescence (pâ¯>â¯.05). In the SP, the proportion of NPY-IR neurons significantly increased in the first month of life from 56.3⯱â¯2.4% in 1-day-old to 78.1⯱â¯5.18% in 20-day-old and significantly decreased from 75.6⯱â¯4.62% in 30-day-old rats to 59.8⯱â¯4.24% in 2-year-old rats. The expression of NPY in the duodenum did not change significantly during the development by western blot analysis. The expression of Y1R and Y2R was low in newborns and upregulated in the first ten days of life. The expression of Y5R was maximal in newborn pups and significantly decreased in in the first 20â¯days. Thus, there are some fluctuation of the percentage of NPY-IR neurons accompanies changes in relation of different subtypes of NPY receptors in the small intestine during postnatal ontogenesis.