Wheel-Running Exercise Alleviates Anxiety-Like Behavior via Down-Regulating S-Nitrosylation of Gephyrin in the Basolateral Amygdala of Male Rats.

Physical exercise has beneficial effect on anxiety disorders, but the underlying molecular mechanism remains largely unknown. Here, it is demonstrated that physical exercise can downregulate the S-nitrosylation of gephyrin (SNO-gephyrin) in the basolateral amygdala (BLA) to exert anxiolytic effects. It is found that the level of SNO-gephyrin is significantly increased in the BLA of high-anxiety rats and a downregulation of SNO-gephyrin at cysteines 212 and 284 produced anxiolytic effect. Mechanistically, inhibition of SNO-gephyrin by either Cys212 or Cys284 mutations increased the surface expression of GABAAR γ2 and the subsequent GABAergic neurotransmission, exerting anxiolytic effect in male rats. On the other side, overexpression of neuronal nitric oxide synthase in the BLA abolished the anxiolytic-like effects of physical exercise. This study reveals a key role of downregulating SNO-gephyrin in the anxiolytic effects of physical exercise, providing a new explanation for protein post-translational modifications in the brain after exercise.

Achieving Reliable and Ultrafast Memristors via Artificial Filaments in Silk Fibroin.

The practical implementation of memristors in neuromorphic computing and biomimetic sensing suffers from unexpected temporal and spatial variations due to the stochastic formation and rupture of conductive filaments (CFs). Here, the biocompatible silk fibroin (SF) is patterned with an on-demand nanocone array by using thermal scanning probe lithography (t-SPL) to guide and confine the growth of CFs in the silver/SF/gold (Ag/SF/Au) memristor. Benefiting from the high fabrication controllability, cycle-to-cycle (temporal) standard deviation of the set voltage for the structured memristor is significantly reduced by ≈95.5% (from 1.535 to 0.0686 V) and the device-to-device (spatial) standard deviation is also reduced to 0.0648 V. Besides, the statistical relationship between the structural nanocone design and the resultant performance is confirmed, optimizing at the small operation voltage (≈0.5 V) and current (100 nA), ultrafast switching speed (sub-100 ns), large on/off ratio (104 ), and the smallest switching slope (SS < 0.01 mV dec-1 ). Finally, the short-term plasticity and leaky integrated-and-fire behavior are emulated, and a reliable thermal nociceptor system is demonstrated for practical neuromorphic applications.

Brush-like gold nanowires-anchored g-C3N4 nanosheets with tunable geometry for ultrasensitive and regenerative SERS detection of gaseous molecules.

Strapping plasmonic substrate with a reliable ability to anchor molecules and achieve reproducible result provides trustworthy opportunities for flourishing surface-enhanced Raman scattering (SERS) technique. Herein, a facile controllable in-situ anisotropic growth strategy was exploited to anchor gold nanowires (Au NWs) onto two-dimensional g-C3N4 nanosheets (g-C3N4/Au NWs), facilitating a sensitive and recyclable SERS sensor for gaseous analytes. Benefiting from the attractive enrichment effect of the brush-like surface formed by numerous small Au NWs as well as their rich nanotips-mediated enhancement capability, the hybrid substrate showed an outstanding performance in SERS-based detection of trace 4-Aminothiophenol (4-ATP) molecules, demonstrating a monitoring limitation down to 10-8 M even in atmosphere. Satisfyingly, under visible light illumination, the efficient green photocatalytic ability derived from the g-C3N4 supporting matrix rendered reusable capability for the substrate, whose SERS signal was kept at a persistent high level throughout 6 cycles. Attributed to the narrow line width of SERS spectrum, the 4-ATP assay under the interference of 2-naphthalenethiol (2-NAT) was acquired in gas phase and the dependable recovery rates from 85.4 to 93.9% were confirmed as well. Thanks to the intriguing features including excellent sensitivity and recyclability, the g-C3N4/Au NWs substrate proposed here will pave the way toward the potential application of SERS technique in multiplexed gaseous detection.

Recyclable surface enhanced Raman scattering monitoring of nucleotides and their metabolites based on Au nanoflowers modified g-C3N4 nanosheets.

The effective monitoring of nucleotides and their metabolites is critical for the prevention of various genetic metabolic diseases. In this aspect, surface-enhanced Raman scattering (SERS) as an ultrasensitive and nondestructive sensing techniques has spurted an attractive prospect. Henceforth, an effective SERS-based analysis of adenosine monophosphate (AMP) as well as adenine was realized here by utilizing an innovative kind of self-cleaning substrate constructed with graphitic carbon nitride and Au nanoflowers (g-C3N4@Au NFs). Taking advantages of the numerous nanotip-triggered "hotspot" regions of Au NFs as well as the excellent photocatalytic degradation capability of g-C3N4 matrix, the hybrid substrate not only brought about a low limit of detection (LOD) of 5.01 × 10-10 M, but also yielded an attractive self-cleaning property. Particularly, these fascinating features of the proposed g-C3N4@Au NFs facilitated a recyclable monitoring of AMP and adenine in serum with reliable sensitivity and stability. The achieved wide linear range from 10-4 to 1 mg/mL together with the LOD values down to 10-5 mg/mL all envisioned that it is possible to monitor and investigate human metabolic processes by SERS protocol.

Multifunctional SERS chip mediated by black phosphorus@gold-silver nanocomposites inserted in bilayer membrane for in-situ detection and degradation of hazardous materials.

Self-cleaning surface-enhanced Raman scattering (SERS) substrates dependent on versatile two-dimensional semiconductors offer an efficient channel for the sensitive monitoring and timely degradation of hazardous molecules. Herein, a kind of sophisticated SERS-active nanocomposites was developed by incorporating Au-Ag nanoparticles onto black phosphorus (BP) nanosheets via photo-induced self-reduction. Combining the substantial electromagnetic "hot spots" triggered by bimetallic plasma coupling effect and the efficient charge transfer from BP to probe molecules, the proposed nanocomposites featured attractive SERS enhancement, facilitating a limit of detection down to 4.5 × 10-10 M. Attributed to the remarkable restriction of electron-hole recombination stemming from "Schottky contact", the photocatalytic activity of BP was prominently boosted, demonstrating a complete degradation time as short as 65 min. Furthermore, the disgusting instability of BP was considerably hindered by inserting the nanocomposites into various bilayer matrices with diverse hardness and viscosity inspired by cling film principle. Moreover, a significantly elevated collection rate high to 93.1% for in-situ detection was also achieved by the as-manufactured flexible SERS chips based on tape. This study illustrates a clear perspective for the development of versatile BP-based SERS chips which might facilitate sensitive analysis and treatment of perilous contaminants in complicated real-life scenarios.

Quantitative SERS sensing mediated by internal standard Raman signal from silica nanoparticles in flexible polymer matrix.

Attributed to poor signal uniformity and external interference, ultrasensitive surface-enhanced Raman spectroscopy (SERS) still faces difficulties in the reliable and quantitative detection of trace molecules. Here, a facile Ag/Si/sodium carboxy methyl cellulose (NaCMC) film with internal standard (IS) was promoted for quantitative determination of thiram. The effects of preparation conditions on SERS activity of the film were systematically investigated and then a flexible SERS substrate with high sensitivity and uniformity was fabricated. The enhancement factor was calculated to be 1.12 × 106 and SERS mapping was recorded with a relative standard deviation value of 19.8% by utilizing 4-mercaptobenzoic acid (4-MBA) as target molecule. Additionally, the dominant contribution of the IS from encapsulated Si nanoparticles (NPs) was confirmed in the quantitative assay of 4-MBA and thiram, facilitating attractive fitting coefficients (R2) as 0.991 and 0.998. Besides that, the proposed flexible film was conducted to scrub trace thiram from the surfaces of apple, orange, and cucumber, resulting in recoveries of 89%, 94%, and 91%. A smart and facile quantitative SERS substrate was developed here for monitoring trace biochemical molecules, verifying its potential utilizations in monitoring pesticide residues.

Reusable dual-functional SERS sensor based on gold nanoflowers-modified red phosphorus nanoplates for ultrasensitive immunoassay and degradation of CA19-9.

It is tremendously desirable for the timely and effective detection of cancer to facilitate the ultra-highly sensitive monitoring of tumor marker in clinical serum sample. In this study, an electromagnetic and chemical synergistically enabled recyclable immunoassay based on surface-enhanced Raman scattering (SERS) was proposed by realizing the anisotropic growth of sea-urchin-like gold nanoflowers (Au NFs) on two-dimensional red phosphorus (RP) nanoplates. Besides the achieved enhancement factor as high as 2.24 × 106, it was found that the photocatalytic and SERS activities were kept at a high level for the hybrid substrate of RP/Au NFs throughout 7 cycles of immunoassay. In combination with a non-metallic immunoprobe, the limit of detection was drove to 7.41 × 10-5 IU·mL-1 for cancer antigen 19-9. The comparative experiments of nonspecific monitoring verified the promising selectivity of this strategy. Considering the intriguing features of high sensitivity, recyclability, and specificity, the proposed multifunctional RP/Au NFs exhibited its superior role in the early detection of cancer and can be adapted for point-of-care diagnosis.