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This study aimed to develop an intranasal nanovaccine by combining chiral nanoparticles with the RSV pre-fusion protein (RSV protein) to create L-nanovaccine (L-Vac). The results showed that L-NPs increased antigen attachment in the nasal cavity by 3.83 times and prolonged its retention time to 72 h. In vivo experimental data demonstrated that the intranasal immunization with L-Vac induced a 4.86-fold increase in secretory immunoglobulin A (sIgA) secretion in the upper respiratory tract, a 1.85-fold increase in the lower respiratory tract, and a 20.61-fold increase in RSV-specific immunoglobin G (IgG) titer levels in serum, compared with the commercial Alum Vac, while the neutralizing activity against the RSV authentic virus is 1.66-fold higher. The mechanistic investigation revealed that L-Vac activated the tumor necrosis factor (TNF) signaling pathway in nasal epithelial cells (NECs), in turn increasing the expression levels of interleukin-6 (IL-6) and C-C motif chemokine ligand 20 (CCL20) by 1.67-fold and 3.46-fold, respectively, through the downstream nuclear factor kappa-B (NF-κB) signaling pathway. Meanwhile, CCL20 recruited dendritic cells (DCs) and L-Vac activated the Toll-like receptor signaling pathway in DCs, promoting IL-6 expression and DCs maturation, and boosted sIgA production and T-cell responses. The findings suggested that L- Vac may serve as a candidate for the development of intranasal medicine against various types of respiratory infections.
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In this study, we used Fe-MIL-101 nanozyme to convert lactose into lactitol, and it was proved that Fe-MIL-101 nanozyme has lactase-like activity. Due to the potential health effects of nanomaterials, we evaluated the cytotoxicity of Fe-MIL-101 nanozyme. To reduce the potential toxicity of the nanozyme, we applied centrifugation and membrane filtration. When the membrane aperture size was 100 nm, the residual content of Fe-MIL-101 nanozyme was 14.09 µg/mL. The residual content of Fe-MIL-101 nanozyme was reduced by optimizing time, temperature, and Fe-MIL-101 nanozyme-to-substrate ratio. It was showed that the concentration of Fe was 38.47 mg/kg and the concentration of H2BDC was 0 mg/kg under optimized conditions (110â, 2 h of reaction and the ratio of Fe-MIL-101 nanozyme to substrate is 1:20). The result met the national standard of China. Experiments measuring cytotoxicity, oxidative stress, and cell membrane damage revealed that less than 20 µg/mL Fe-MIL-101 nanozyme had no significant cytotoxicity. Our study findings showed that Fe-MIL-101 nanozyme reduced lactose content in milk.
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Tricyclazole is commonly used to prevent rice blast to meet the carbohydrate intake needs of half of the global population, and a large number of toxicological reports indicate that monitoring of tricyclazole is necessary. Here, we analyzed the structure of tricyclazole and designed different hapten derivatization strategies to prepare a high-performance monoclonal antibody (half inhibition concentration of 1.61 ng/mL), and then a lateral flow immunochromatographic sensor based on gold nanoparticles for the detection of tricyclazole in rice, with a limit of detection of 6.74 µg/kg and 13.58 µg/kg in polished and brown rice, respectively. The recoveries in rice were in the range of 84.6-107.4%, no complex pretreatment was required for comparison with LC-MS/MS, and the comparative analysis demonstrated that our method had good accuracy and precision. Therefore, the developed lateral flow immunochromatographic analysis was a reliable and rapid means for the on-site analysis of tricyclazole in rice.
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Cromatografía de Afinidad , Contaminación de Alimentos , Oryza , Oryza/química , Contaminación de Alimentos/análisis , Cromatografía de Afinidad/instrumentación , Cromatografía de Afinidad/métodos , Tiazoles/análisis , Tiazoles/química , Anticuerpos Monoclonales/química , Anticuerpos Monoclonales/análisis , Límite de Detección , Fungicidas Industriales/análisis , Inmunoensayo/métodos , Inmunoensayo/instrumentación , Residuos de Plaguicidas/análisis , Oro/químicaRESUMEN
Bupirimate (BPM) is a high-efficiency and low-toxicity fungicide used to combat powdery mildew in crops. To mitigate potential health risks to consumers resulting from improper BPM usage, we prepared a monoclonal antibody against BPM based on novel hapten synthesis, which has high sensitivity and strong specificity, and then successfully designed a colloidal gold-based immunochromatographic (ICG) strip. The newly designed ICG strip was then employed for detecting BPM residues in peach, orange, and carrot. The results show that for the peach, orange, and carrot samples, the calculated detection limits of the ICG strip are 9.36, 0.79, and 0.57 ng/g, respectively, and that it is resistant to the matrix effect and meets the maximum residue limit requirements of European Commission for BPM. Therefore, this developed ICG strip is expected to enable swift detection of BPM residues on the spot.
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Cromatografía de Afinidad , Citrus sinensis , Daucus carota , Contaminación de Alimentos , Residuos de Plaguicidas , Prunus persica , Contaminación de Alimentos/análisis , Daucus carota/química , Prunus persica/química , Cromatografía de Afinidad/métodos , Cromatografía de Afinidad/instrumentación , Citrus sinensis/química , Residuos de Plaguicidas/análisis , Fungicidas Industriales/análisis , Anticuerpos Monoclonales/análisis , Límite de DetecciónRESUMEN
Asymmetric transformations and synthesis have garnered considerable interest in recent decades due to the extensive need for chiral organic compounds in biomedical, agrochemical, chemical, and food industries. The field of chiral inorganic catalysts, garnering considerable interest for its contributions to asymmetric organic transformations, has witnessed remarkable advancements and emerged as a highly innovative research area. Here, we review the latest developments in this dynamic and emerging field to comprehensively understand the advances in chiral inorganic nanocatalysts and stimulate further progress in asymmetric catalysis.
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Chiral nanoscale enantiomers exhibit different biological effects in living systems. However, their chirality effect on the detection sensitivity for chiral biological targets still needs to be explored. Here, we discovered that Co2+ can modulate the luminescence performance of L/D-glutathione (GSH)-modified copper nanoclusters (L/D-Cu NCs) and induce strong chiroptical activities as the asymmetric factor was enhanced 223-fold with their distribution regulating from the ultraviolet to visible region. One Co2+ coordinated with two GSH molecules that modified on the surface of Cu NCs in the way of CoN2O2. On this basis, dual-modal chiral and luminescent signals of Co2+ coordinated L/D-Cu NCs (L/D-Co-Cu NCs) were used to detect the chiral adenosine triphosphate (ATP) based on the competitive interaction between surficial GSH and ATP molecules with Co2+. The limits of detection of ATP obtained with fluorescence and circular dichroism intensity were 9.15â µM and 15.75â nM for L-Co-Cu NCs, and 5.35â µM and 4.69â nM for D-Co-Cu NCs. This demonstrated that selecting suitable chiral configurations of nanoprobes effectively enhances detection sensitivity. This study presents not only a novel method to modulate and enhance the chiroptical activity of nanomaterials but also a unique perspective of chirality effects on the detection performances for bio-targets.
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Cobre , Nanoestructuras , Adenosina Trifosfato , Luminiscencia , GlutatiónRESUMEN
Contamination of oilfield chemicals (OFCs) by benzo[a]pyrene (B[a]P) is increasingly becoming a severe environmental security issue. There is an urgent need to develop a rapid and accurate method for B[a]P detection in OFCs. In this study, B[a]P hapten was designed using computer aided molecular design. A high-affinity, specific, and matrix-insensitive monoclonal antibody (mAb) with IC50 values of 6.77 ng/mL was obtained. Based on this mAb, we developed a rapid gold nanoparticle-based immunochromatographic strip assay (GICA) with double T-line mode for on-site detection of B[a]P in OFCs samples. The GICA exhibited excellent detection performance in OFCs samples with strong acidity, strong alkalinity, and deep color. Under optimal conditions, the proposed method detected B[a]P in OFCs at 0.42-300 mg/kg, and limit of detection was 0.23-1.07 mg/kg. The recovery rate was 88-106% with a coefficient of variation of 1.46-6.35%. Confirmed by natural positive OFCs samples and high-performance liquid chromatography, this GICA is accurate and reliable, with great potential for rapid and cost-effective on-site detection.
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Oro , Nanopartículas del Metal , Oro/química , Benzo(a)pireno , Análisis Costo-Beneficio , Yacimiento de Petróleo y Gas , Nanopartículas del Metal/química , Cromatografía de Afinidad , Inmunoensayo/métodos , Anticuerpos Monoclonales , Límite de DetecciónRESUMEN
The incessant mutations of viruses, variable immune responses, and likely emergence of new viral threats necessitate multiple approaches to novel antiviral therapeutics. Furthermore, the new antiviral agents should have broad-spectrum activity and be environmentally stable. Here, we show that biocompatible tapered CuS nanoparticles (NPs) efficiently agglutinate coronaviruses with binding affinity dependent on the chirality of surface ligands and particle shape. L-penicillamine-stabilized NPs with left-handed curved apexes display half-maximal inhibitory concentrations (IC50) as low as 0.66 pM (1.4 ng/mL) and 0.57 pM (1.2 ng/mL) for pseudo-type SARS-CoV-2 viruses and wild-type Wuhan-1 SARS-CoV-2 viruses, respectively, which are about 1,100 times lower than those for antibodies (0.73 nM). Benefiting from strong NPs-protein interactions, the same particles are also effective against other strains of coronaviruses, such as HCoV-HKU1, HCoV-OC43, HCoV-NL63, and SARS-CoV-2 Omicron variants with IC50 values below 10 pM (21.8 ng/mL). Considering rapid response to outbreaks, exposure to elevated temperatures causes no change in the antiviral activity of NPs while antibodies are completely deactivated. Testing in mice indicates that the chirality-optimized NPs can serve as thermally stable analogs of antiviral biologics complementing the current spectrum of treatments.
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COVID-19 , Coronavirus Humano OC43 , Humanos , Animales , Ratones , SARS-CoV-2/genética , Anticuerpos/farmacología , Antivirales/farmacología , Antivirales/uso terapéuticoRESUMEN
Excessive accumulation of reactive oxygen species (ROS) can lead to oxidative stress and oxidative damage, which is one of the important factors for aging and age-related diseases. Therefore, real-time monitoring and the moderate elimination of ROS is extremely important. In this study, a ROS-responsive circular dichroic (CD) at 553 nm and magnetic resonance imaging (MRI) dual-signals chiral manganese oxide (MnO2 ) nanoparticles (NPs) are designed and synthesized. Both the CD and MRI signals show excellent linear ranges for intracellular hydrogen peroxide (H2 O2 ) concentrations, with limits of detection (LOD) of 0.0027 nmol/106 cells and 0.016 nmol/106 cells, respectively. The lower LOD achieved with CD detection may be attributable to its higher anti-interference capability from the intracellular matrix. Importantly, ROS-induced cell aging is intervened by chiral MnO2 NPs via redox reactions with excessive intracellular ROS. In vivo experiments confirm that chiral MnO2 NPs effectively eliminate ROS in skin tissue, reduce oxidative stress levels, and alleviate skin aging. This approach provides a new strategy for the diagnosis and treatment of age-related diseases.
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Nanopartículas , Óxidos , Especies Reactivas de Oxígeno , Compuestos de Manganeso , Peróxido de HidrógenoRESUMEN
Chirality is a fundamental characteristic of living organisms and is commonly observed at the biomolecule, cellular, and tissue levels. Chiral nanomaterials play an irreplaceable role in nanomedicine and nanobiology because of their unique enantioselectivity with biological components. Here, research progress relating to chiral nanomaterials in the field of vaccines is reviewed, including antigen presenting systems, immune adjuvants, and cancer vaccines. First, the common synthesis methods are outlined for different types of chiral nanomaterials, as well as their chiral sources, optical properties, and potential biological applications. Then, the application of chiral nanomaterials are discussed in the field of vaccines with reference to the promotion of antigen presentation and activation of the immune system for tumor immunotherapy. Finally, the current obstacles and future research directions of chiral nanomaterials are revealed with regard to regulating the immune system.
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Vacunas contra el Cáncer , Nanoestructuras , Neoplasias , Vacunas contra el Cáncer/uso terapéutico , Nanoestructuras/uso terapéutico , Adyuvantes Inmunológicos/uso terapéutico , Presentación de Antígeno , Antígenos , Neoplasias/terapiaRESUMEN
Herein, four haptens of niacin (Vitamin B3, VB3) were designed, and after a series of experiments, it was concluded that hapten D had the best immune effect. To avoid false positives in the detection of real samples, a monoclonal antibody (mAb) against VB3 was prepared by a matrix effect-enhanced mAb screening method. The concentration of the inhibition rate reaching 50% (IC50) was 603.41 ng mL-1 and the limit of detection (LOD) using an indirect enzyme-linked immunosorbent assay (ic-ELISA) was 54.89 ng mL-1. A lateral flow immunochromatographic assay (LFIA) based on gold nanoparticles was established to detect the concentration of VB3 in compound vitamin B tablets and infant formulas, with a visual LOD of 5 µg mL-1. Using a handheld reader, the quantitative LOD was calculated to be 0.60 µg mL-1. The contents of the compound vitamin B tablets and infant formulas were also verified by liquid chromatography. Therefore, the LFIA developed in this study can be applied to the specific identification and rapid detection of niacin in nutritional dietary supplements, thus meeting the market's demand for efficient niacin detection methods.
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Nanopartículas del Metal , Niacina , Lactante , Humanos , Oro/química , Nanopartículas del Metal/química , Anticuerpos Monoclonales , Suplementos Dietéticos , VitaminasRESUMEN
Titanium dioxide (TiO2) has attracted significant attention in the fields of antibacterial activity and pollutant degradation due to its well-known photocatalytic properties. However, the application of TiO2 is significantly limited by its large band gap width, which only allows excitation by ultraviolet light below 400 nm. Here, we propose the use of surface chiral functionalization of TiO2 to tune its band gap width, thus enabling it to be excited by near-infrared-region light (NIR), resulting in the effective separation of electron-hole pairs. By controlling the solvent polarity and forming numerous weak interactions (such as hydrogen bonding) between chiral ligands and TiO2, we successfully prepared chiral TiO2 superparticles (SPs) that exhibited a broad circular dichroism (CD) absorption at 792 nm. Under circularly polarized light (CPL) at 808 nm, the chiral SPs induced the separation of electron-hole pairs in TiO2, thus generating hydroxyl and singlet oxygen radicals. Antibacterial tests under CPL in NIR showed that the chiral TiO2 SPs exhibited excellent antibacterial performance, with inhibition rates of 99.4% and 100% against Gram-positive and Gram-negative bacteria, respectively. Recycling-reuse experiments and biocompatibility evaluation of the material demonstrated that the chiral TiO2 SPs are stable and safe antibacterial materials, thus indicating the potential application of chiral TiO2 SPs in antibacterial aspects of medical implants.
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Antibacterianos , Bacterias Gramnegativas , Antibacterianos/farmacología , Bacterias Grampositivas , Rayos Ultravioleta , Titanio/farmacologíaRESUMEN
Alzheimer's disease (AD) is characterized by amyloid-ß accumulation in the brain and hyperphosphorylated tau aggregation, as well as neuroinflammation. The gut-brain axis has emerged as a therapeutic target in neurodegenerative diseases by modulating metabolic activity, neuroimmune functions and sensory neuronal signaling. Here we investigate interactions between orally ingested chiral Au nanoparticles and the gut microbiota in AD mice. Oral administration of chiral Au nanoparticles restored cognitive abilities and ameliorated amyloid-ß and hyperphosphorylated tau pathologies in AD mice via alterations in the gut microbiome composition and an increase in the gut metabolite, indole-3-acetic acid, which was lower in serum and cerebrospinal fluid of patients with AD compared with age-matched controls. Oral administration of indole-3-acetic acid was able to penetrate the blood-brain barrier and alleviated cognitive decline and pathology including neuroinflammation in AD mice. These findings provide a promising therapeutic target for the amelioration of neuroinflammation and treatment of neurodegenerative diseases.
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Enfermedad de Alzheimer , Microbioma Gastrointestinal , Nanopartículas del Metal , Humanos , Ratones , Animales , Microbioma Gastrointestinal/fisiología , Eje Cerebro-Intestino , Enfermedades Neuroinflamatorias , Oro , Nanopartículas del Metal/uso terapéutico , Enfermedad de Alzheimer/patología , Péptidos beta-Amiloides/metabolismoRESUMEN
Imazalil (IMZ) is a commonly used fungicide for controlling fungus in agriculture, leaving residual IMZ in crops that could be hazardous to human health. In this work, we designed IMZ haptens for mice immunization and prepared sensitive monoclonal antibody (mAb) against IMZ. The subtype of anti-IMZ mAb is IgG2a. It possessed a half inhibition concentration (IC50) of 0.95 ng mL-1 and showed no cross-reactivity against other chemicals in ic-ELISA. Taking advantage of the mAb, we developed a gold nanoparticle-based immunochromatographic assay (GICA) for the rapid detection of IMZ in grapes and tomatoes. The assay gave a visual limit of detection (vLOD) of 25 ng g-1 and cut-off value of 500 ng g-1 in both samples. According to the calibration curves, the calculated LOD were 4.12 ng g-1 and 4.70 ng g-1 in grapes and tomatoes, respectively. The recovery rates of IMZ ranged from 84.7% to 104.4% with variation coefficients (CVs) of 5.7-11.8% in spiked samples, indicating a potent practicability of the GICA. The whole GICA process took 30 min. Therefore, the developed assay can be used for on-site detection and quantitation of IMZ in grape and tomato samples.
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Oro , Nanopartículas del Metal , Humanos , Animales , Ratones , Cromatografía de Afinidad/métodos , Inmunoensayo/métodos , Anticuerpos Monoclonales/químicaRESUMEN
Here, we develop a novel methodology for synthesizing chiral CdSe@ZnS quantum dots (QDs) with enhanced circularly polarized luminescence (CPL) by incorporating l-/d-histidine (l-/d-His) ligands during ZnS shell growth at the water/oil interface. The resulting chiral QDs exhibit exceptional absolute photoluminescence quantum yield of up to 67.2%, surpassing the reported limits of 40.0% for chiral inorganic QDs, along with absorption dissymmetry factor (|gabs|) and luminescence dissymmetry factor (|glum|) values of 10-2, exceeding the range of 10-5-10-3 and 10-4-10-2, respectively. Detailed investigations of the synthetic pathway reveal that the interface, as a binary synthetic environment, facilitates the coordinated ligand exchange and shell growth mediated by chiral His-Zn2+ coordination complexes, leading to a maximum fluorescent brightness and chiroptical activities. The growth process, regulated by the His-Zn2+ coordination complex, not only reduces trap states on the CdSe surface, thereby enhancing the fluorescence intensity, but also significantly promotes the orbital hybridization between QDs and chiral ligands, effectively overcoming the shielding effect of the wide bandgap shell and imparting pronounced chirality. The proposed growth pathway elucidates the origin of chirality and provides insights into the regulation of the CPL intensity in chiral QDs. Furthermore, the application of CPL QDs in multilevel anticounterfeiting systems overcomes the limitations of replication in achiral fluorescence materials and enhances the system's resistance to counterfeiting, thus opening new opportunities for chiral QDs in optical anticounterfeiting and intelligent information encryption.
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Here, we report the synthesis of chiral selenium nanoparticles (NPs) using cysteine and the interfacial assembly strategy to generate a self-assembled nanomembrane on a large-scale with controllable morphology and handedness. The selenide (Se) NPs exhibited circular dichroism (CD) bands in the ultraviolet and visible region with a maximum intensity of 39.96â mdeg at 388â nm and optical anisotropy factors (g-factors) of up to 0.0013 while a self-assembled monolayer nanomembrane exhibited symmetrical CD approaching 72.8â mdeg at 391â nm and g-factors up to 0.0034. Analysis showed that a photocurrent of 20.97±1.55â nA was generated by the D-nanomembrane when irradiated under light while the L-nanomembrane generated a photocurrent of 20.58±1.36â nA. Owing to the asymmetric intensity of the photocurrent with respect to the handedness of the nanomembrane, an ultrasensitive recognition of enantioselective kynurenine (Kyn) was achieved by the ten-layer (10L) D-nanomembrane exhibiting a photocurrent for L-kynurenine (L-Kyn) that was 8.64-fold lower than that of D-Kyn, with a limit of detection (LOD) of 0.0074â nM for the L-Kyn, which was attributed to stronger affinity between L-Kyn and D-Se NPs. Noticeably, the chiral Se nanomembrane precisely distinguished L-Kyn in serum and cerebrospinal fluid samples from Alzheimer's disease patients and healthy subjects.
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The chemical, physical and biological effects of chiral nanomaterials have inspired general interest and demonstrated important advantages in fundamental science. Here, chiral iron oxide supraparticles (Fe3 O4 SPs) modified by chiral penicillamine (Pen) molecules with g-factor of ≈2 × 10-3 at 415 nm are fabricated, and these SPs act as high-quality magnetic resonance imaging (MRI) contrast agents. Therein, the transverse relaxation efficiency and T2 -MRI results demonstrated chiral Fe3 O4 SPs have a r2 relaxivity of 157.39 ± 2.34 mM-1 ·S-1 for D-Fe3 O4 SPs and 136.21 ± 1.26 mM-1 ·S-1 for L-Fe3 O4 SPs due to enhanced electronic transition dipole moment for D-Fe3 O4 SPs compared with L-Fe3 O4 SPs. The in vivo MRI results show that D-Fe3 O4 SPs exhibit two-fold lower contrast ratio than L-Fe3 O4 SPs, which enhances targeted enrichment in tumor tissue, such as prostate cancer, melanoma and brain glioma tumors. Notably, it is found that D-Fe3 O4 SPs have 7.7-fold higher affinity for the tumor cell surface receptor cluster-of-differentiation 47 (CD47) than L-Fe3 O4 SPs. These findings uncover that chiral Fe3 O4 SPs act as a highly effective MRI contrast agent for targeting and imaging broad tumors, thus accelerating the practical application of chiral nanomaterials and deepening the understanding of chirality in biological and non-biological environments.
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Neoplasias Encefálicas , Glioma , Masculino , Humanos , Compuestos Férricos , Medios de Contraste , Glioma/diagnóstico por imagen , Imagen por Resonancia Magnética/métodosRESUMEN
Traumatic brain injury has become a serious public health problem. Timely detection, diagnosis and treatment of brain injury are closely related to the prognosis of patients, so identification of highly sensitive and specific biochemical markers of brain injury has important clinical value. Currently, the most studied and most promising marker is the protein S100B. In this study, a rapid quantitative biosensor for S100B was established using colloidal gold labeling and double antibody (8C10-6B8) sandwich immunochromatography. The biosensor was capable of quantifying S100B within 15 min, and showed no cross-reactivity with S100A, NSE, GFAP, or PGP9.5. The detection limit was determined to be 4.6 pg mL-1 with a linear range of 0.01-2 ng mL-1. Recovery experiments also indicated that the method had an acceptable accuracy. Moreover, the quantitative colloidal gold assay correlated well with the results of a chemiluminescence immunoassay when testing 40 clinical serum samples. Our developed colloidal gold quantitative immunochromatographic biosensor is a rapid, sensitive, specific and accurate method for the detection of S100B protein in serum, which is useful in the clinic for early diagnosis, as well as assessment of disease progression and prognosis of traumatic brain injury.
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Técnicas Biosensibles , Lesiones Traumáticas del Encéfalo , Lesiones Encefálicas , Humanos , Oro Coloide/química , Lesiones Traumáticas del Encéfalo/diagnóstico , Cromatografía de Afinidad/métodos , Subunidad beta de la Proteína de Unión al Calcio S100RESUMEN
Chiral nanomaterials with different functions have been widely developed, but the deep understanding of the structural effects of nanocatalysts on enantioselective photocatalytic efficiency is still highly demanded. Herein, Pt and Pt-Au-bimetal-doped chiral nanostructures with various morphologies and compositions are facilely constructed using L-/D-arginine (L-/D-Arg) and mono-sulfonate tetraphenyl porphyrin (H2TPPS) assemblies as chiral templates. Interestingly, these Pt and Pt-Au-doped chiral nanostructures, including nanorods (NR) and nanospheres (NS), can be well regulated by controlling pH, ionic strength, and reaction time of the assembling system of Arg and H2TPPS. More impressively, specific Au growth direction along the Pt-doped chiral NR (L-/D-Pt-NR) is observed (from tip to middle) during the preparation of Pt-Au-bimetal-doped chiral NR (L-/D-Pt-Au-NR) and their compositions can be finely controlled by simply adjusting the concentrations of HAuCl4. As expected, the chiral nanostructures exhibit superior enantioselective photocatalytic ability toward chiral organics under visible light: the oxidation rate of L-dihydroxy-phenylalanine (L-DOPA) catalyzed by L-Pt-NR (or D-DOPA catalyzed by D-Pt-NR) is about 60% higher than that of L-DOPA catalyzed by D-Pt-NR (or L-DOPA catalyzed by D-Pt-NR). This study provides a facile strategy to construct chiral nanostructures for the photocatalytic conversion of chiral organics.
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In the study, a hapten was designed to preserve the molecular structure of tolfenpyrad while introducing a carboxyl group and was coupled with a carrier protein to synthesize an immunogen and coating antigen. A monoclonal antibody was fabricated against tolfenpyrad and its performance was assessed by indirect competitive enzyme-linked immunosorbent assay. Finally, we developed a colloidal gold nanoparticle immunochromatographic test strip (CGN-ICTS) for the detection of tolfenpyrad in kale, Chinese cabbage, and eggplant samples. The results shows that CGN-ICTS was sensitive, with calculated detection limits of 0.49 ng/g for kale and Chinese cabbage and 0.99 ng/g for eggplant. Subsequently, CGN-ICTS and LC-MS were used to analyze the tolfenpyrad-spiked samples. The recovery rate of the CGN-ICTS for kale samples was 97.1-103.0%, for Chinese cabbage samples was 93.7-103.4%, and for eggplant samples was 92.7-105.7%. Recovery rates were similar between CGN-ICTS and LC-MS. Therefore, CGN-ICTS can be used to quickly screen tolfenpyrad residues in foods.