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1.
Biosens Bioelectron ; 267: 116757, 2024 Sep 07.
Artículo en Inglés | MEDLINE | ID: mdl-39250871

RESUMEN

Plant signaling molecules can be divided into plant messenger signaling molecules (such as calcium ions, hydrogen peroxide, Nitric oxide) and plant hormone signaling molecules (such as auxin (mainly indole-3-acetic acid or IAA), salicylic acid, abscisic acid, cytokinin, jasmonic acid or methyl jasmonate, gibberellins, brassinosteroids, strigolactone, and ethylene), which play crucial roles in regulating plant growth and development, and response to the environment. Due to the important roles of the plant signaling molecules in the plants, many methods were developed to detect them. The development of in-situ and real-time detection of plant signaling molecules and field-deployable sensors will be a key breakthrough for botanical research and agricultural technology. Electrochemical methods provide convenient methods for in-situ and real-time detection of plant signaling molecules in plants because of their easy operation, high sensitivity, and high selectivity. This article comprehensively reviews the research on electrochemical detection of plant signaling molecules reported in the past decade, which summarizes the various types electrodes of electrochemical sensors and the applications of multiple nanomaterials to enhance electrode detection selectivity and sensitivity. This review also provides examples to introduce the current research trends in electrochemical detection, and highlights the applicability and innovation of electrochemical sensors such as miniaturization, non-invasive, long-term stability, integration, automation, and intelligence in the future. In all, the electrochemical sensors can realize in-situ, real-time and intelligent acquisition of dynamic changes in plant signaling molecules in plants, which is of great significance for promoting basic research in botany and the development of intelligent agriculture.

2.
PhytoKeys ; 220: 59-64, 2023.
Artículo en Inglés | MEDLINE | ID: mdl-37251609

RESUMEN

A new species Veronicahongii, from western Hubei Province, Central China is described and illustrated. The species is morphologically similar to V.henryi Yamazaki, but mainly differs in the glabrous plant, except pedicels, broadly ovate leaf blades, glandular-pubescent pedicels, obovate calyx lobes, smaller corolla, broadly ovate capsule and much smaller seeds.

3.
PhytoKeys ; 220: 51-57, 2023.
Artículo en Inglés | MEDLINE | ID: mdl-37251616

RESUMEN

Based on examination of syntype specimens deposited at P, the lectotype for the name Deutziasetchuenensis Franch. is designated here. By consulting literature and specimen records, the type locality of D.setchuenensisvar.longidentata Rehder, 'Chin-Ting shan' in the protologue is likely a misspelling of 'Chiuting shan' which is now called Jiuding shan located in southern Mao county, Sichuan province. In addition, a new variety, Deutziasetchuenensisvar.macrocarpa Q.L.Gan, Z.Y.Li & S.Z.Xu from western Hubei, Central China, is described and illustrated. It differs from other varieties of D.setchuenensis Franch. by the orange anthers, broader outer filaments, obtuse inner filaments, and larger fruits.

4.
PhytoKeys ; 219: 49-55, 2023.
Artículo en Inglés | MEDLINE | ID: mdl-37252451

RESUMEN

A new species Astragalusbashanensis, from western Hubei Province, Central China is described and illustrated. The new species is morphologically similar to Astragalussinicus and A.wulingensis, but differs from both by its spreading pubescent indumentum on stems and petioles, longer petioles, white bracts, whitish or yellow corolla, longer claw of the keel-petal, hairy pods and smaller seeds.

5.
Genes (Basel) ; 13(11)2022 11 02.
Artículo en Inglés | MEDLINE | ID: mdl-36360252

RESUMEN

NAC (NAM/ATAF/CUC) transcription factors belong to a unique gene family in plants, which play vital roles in regulating diverse biological processes, including growth, development, senescence, and in response to biotic and abiotic stresses. Tomato (Solanum lycopersicum), as the most highly valued vegetable and fruit crop worldwide, is constantly attacked by Pseudomonas syringae pv. tomato DC3000 (Pst DC3000), causing huge losses in production. Thus, it is essential to conduct a comprehensive identification of the SlNAC genes involved in response to Pst DC3000 in tomato. In this study, a complete overview of this gene family in tomato is presented, including genome localization, protein domain architectures, physical and chemical features, and nuclear location score. Phylogenetic analysis identified 20 SlNAC genes as putative stress-responsive genes, named SSlNAC 1-20. Expression profiles analysis revealed that 18 of these 20 SSlNAC genes were significantly induced in defense response to Pst DC3000 stress. Furthermore, the RNA-seq data were mined and analyzed, and the results revealed the expression pattern of the 20 SSlNAC genes in response to Pst DC3000 during the PTI and ETI. Among them, SSlNAC3, SSlNAC4, SSlNAC7, SSlNAC8, SSlNAC12, SSlNAC17, and SSlNAC19 were up-regulated against Pst DC3000 during PTI and ETI, which suggested that these genes may participate in both the PTI and ETI pathway during the interaction between tomato and Pst DC3000. In addition, SSlNAC genes induced by exogenous hormones, including indole-3-acetic acid (IAA), abscisic acid (ABA), salicylic acid (SA), and methyl jasmonic acid (MeJA), were also recovered. These results implied that SSlNAC genes may participate in the Pst DC3000 stress response by multiple regulatory pathways of the phytohormones. In all, this study provides important clues for further functional analysis and of the regulatory mechanism of SSlNAC genes under Pst DC3000 stress.


Asunto(s)
Solanum lycopersicum , Regulación de la Expresión Génica de las Plantas/genética , Solanum lycopersicum/metabolismo , Filogenia , Enfermedades de las Plantas/genética , Transducción de Señal/genética
6.
Int J Mol Sci ; 23(19)2022 Sep 21.
Artículo en Inglés | MEDLINE | ID: mdl-36232411

RESUMEN

Fatty acid desaturases (FADs) modulate carbon-carbon single bonds to form carbon-carbon double bonds in acyl chains, leading to unsaturated fatty acids (UFAs) that have vital roles in plant growth and development and their response to environmental stresses. In this study, a total of 23 Populus trichocarpaFAD (PtFAD) candidates were identified from the poplar genome and clustered into seven clades, including FAB2, FAD2, FAD3/7/8, FAD5, FAD6, DSD, and SLD. The exon-intron compositions and conserved motifs of the PtFADs, clustered into the same clade, were considerably conserved. It was found that segmental duplication events are predominantly attributable to the PtFAD gene family expansion. Several hormone- and stress-responsive elements in the PtFAD promoters implied that the expression of the PtFAD members was complicatedly regulated. A gene expression pattern analysis revealed that some PtFAD mRNA levels were significantly induced by abiotic stress. An interaction proteins and gene ontology (GO) analysis indicated that the PtFADs are closely associated with the UFAs biosynthesis. In addition, the UFA contents in poplars were significantly changed under drought and salt stresses, especially the ratio of linoleic and linolenic acids. The integration of the PtFAD expression patterns and UFA contents showed that the abiotic stress-induced PtFAD3/7/8 members mediating the conversion of linoleic and linolenic acids play vital roles in response to osmotic stress. This study highlights the profiles and functions of the PtFADs and identifies some valuable genes for forest improvements.


Asunto(s)
Ácido Graso Desaturasas , Populus , Carbono/metabolismo , Ácido Graso Desaturasas/genética , Ácido Graso Desaturasas/metabolismo , Ácidos Grasos Insaturados/metabolismo , Perfilación de la Expresión Génica , Regulación de la Expresión Génica de las Plantas , Hormonas , Ácidos Linolénicos , Filogenia , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Populus/genética , Populus/metabolismo , ARN Mensajero , Estearoil-CoA Desaturasa/metabolismo , Estrés Fisiológico/genética
7.
Anal Bioanal Chem ; 414(26): 7721-7730, 2022 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-36068347

RESUMEN

The detection of phytohormones in real time has attracted increasing attention because of their critical roles in regulating the development and signaling of plants, especially in defense against biotic stresses. Herein, stainless steel sheet electrodes modified with carbon cement were coupled with paper-based analysis devices for direct and simultaneous detection of salicylic acid (SA) and indole-3-acetic acid (IAA) in plants. We demonstrated that the excellent conductivity of stainless steel sheet electrodes enabled us to simultaneously differentiate IAA and SA at a level of 10 nM. With our approach, the content of IAA and SA in Arabidopsis thaliana leaves infected or not infected with Pst DC3000 could be rapidly quantified at the same time. Our experimental results on differentiation of IAA and SA at different time points showed that there were antagonistic interactions between the IAA and SA after infection of Arabidopsis leaves with Pst DC3000. By offering a cost-effective approach for rapid and sensitive detection of IAA and SA, this study suggests that electrochemical detection can be used in the study and development of precision agriculture technology.


Asunto(s)
Proteínas de Arabidopsis , Arabidopsis , Ácido Salicílico/farmacología , Acero Inoxidable , Reguladores del Crecimiento de las Plantas , Proteínas de Arabidopsis/farmacología , Pseudomonas syringae/fisiología , Ácidos Indolacéticos , Electrodos , Estrés Fisiológico , Carbono/farmacología , Enfermedades de las Plantas
8.
Biosensors (Basel) ; 12(1)2022 Jan 12.
Artículo en Inglés | MEDLINE | ID: mdl-35049663

RESUMEN

As one of the pivotal signal molecules, hydrogen peroxide (H2O2) has been demonstrated to play important roles in many physiological processes of plants. Continuous monitoring of H2O2 in vivo could help understand its regulation mechanism more clearly. In this study, a disposable electrochemical microsensor for H2O2 was developed. This microsensor consists of three parts: low-cost stainless-steel wire with a diameter of 0.1 mm modified by gold nanoparticles (disposable working electrode), an untreated platinum wire with a diameter of 0.1 mm (counter electrode), and an Ag/AgCl wire with a diameter of 0.1 mm (reference electrode), respectively. The microsensor could detect H2O2 in levels from 10 to 1000 µM and exhibited excellent selectivity. On this basis, the dynamic change in H2O2 in the vein of tomato leaf under high salinity was continuously monitored in vivo. The results showed that the production of H2O2 could be induced by high salinity within two hours. This study suggests that the disposable electrochemical microsensor not only suits continuously detecting H2O2 in microscopic plant tissue in vivo but also reduces the damage to plants. Overall, our strategy will help to pave the foundation for further investigation of the generation, transportation, and elimination mechanism of H2O2 in plants.


Asunto(s)
Nanopartículas del Metal , Solanum lycopersicum , Técnicas Electroquímicas , Electrodos , Oro , Peróxido de Hidrógeno/química , Hojas de la Planta , Acero Inoxidable
9.
PhytoKeys ; 199: 1-7, 2022.
Artículo en Inglés | MEDLINE | ID: mdl-36761877

RESUMEN

A new species, Prunuswangii Q.L.Gan, Z.Y.Li & S.Z.Xu from western Hubei, Central China is described and illustrated. It is morphologically similar to P.clarofolia Schneid. and P.pseudocerasus Lindl., but differs in larger height, nearly erect branches, densely and horizontally arranged lenticels, straight lateral veins of leaves, persistent brownish bracts, reflexed and entire calyx lobes, 2-lobed petals with narrowly triangular sinus, earlier flowering and broadly ellipsoid fruits. Furthermore, P.wangii blooms in late February and the colour of flower changes with time, which makes it possible to be a new breeding material for ornamental cherry with early spring blooms.

10.
Mitochondrial DNA B Resour ; 6(7): 2079-2081, 2021 Jun 21.
Artículo en Inglés | MEDLINE | ID: mdl-34212103

RESUMEN

Catalpa fargesii Bur. is endemic to China. Its complete chloroplast genome sequence was firstly reported in this study. The whole chloroplast genome of this species was 157765 bp in length including a pair of inverted repeat (IR, 30252 bp) regions separated by a small single copy (SSC, 12662 bp) and a large single copy (LSC, 84599 bp). The genome consisted of 134 genes, including 89 protein-coding genes, 8 rRNA and 37 tRNA genes. The phylogenetic analysis strongly supported that C. fargesii was closely related to C. fargesii f. duclouxii and C. bungei.

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