RESUMEN
Our previous study confirmed that miR-219-5p inhibits the progression of ovarian cancer (OC) by targeting high mobility group AT-hook 2 (HMGA2), while the role of miR-219-5p on the chemoresistance of OC is unclear. HMGA2 and miR-219-5p expression in OC tumors and various types of OC cells were determined by reverse transcription-quantitative PCR (RT-qPCR) and western blotting. The miRNA profiles in A2780 and cisplatin-resistant A2780 cells were investigated via bulk miRNA sequencing, and the interactions of miR-219-5p and HMGA2 were determined by luciferase reporter activity assay. Cell function was verified through Cell Counting Kit-8, invasion assay, wound-healing, and TUNEL assays. HMGA2 level is highly expressed in cisplatin-resistant OC cell lines compared to normal OC cells, while the expression trend of miR-219-5p is the opposite. In addition, we found that miR-219-5p is one of the miRNAs that have the most significant reduction in levels in the cisplatin-resistant A2780/DDP cell line compared to A2780 cells. Then, we reveal that miR-219-5p directly targets HMGA2 in cisplatin-resistant OC cells, and upregulation of miR-219-5p significantly reduces the resistance of OC cells to cisplatin both in vitro and in vivo. Finally, our results suggest that Wnt/ß-catenin signaling and autophagy pathway is involved in the role of miR-219-5p/HMGA2 on resistance of OC cells to cisplatin via gain-of-function experiments. Collectively, the present study shows that miR-219-5p decreases the resistance of OC cells to cisplatin via Wnt/ß-catenin signaling and autophagy by regulating HMGA2, which provides a feasible solution for the resistance of OC to chemotherapy.
Asunto(s)
MicroARNs , Neoplasias Ováricas , Humanos , Femenino , Cisplatino/farmacología , Cisplatino/uso terapéutico , Neoplasias Ováricas/tratamiento farmacológico , Neoplasias Ováricas/genética , Neoplasias Ováricas/patología , beta Catenina/genética , beta Catenina/metabolismo , Línea Celular Tumoral , Resistencia a Antineoplásicos/genética , MicroARNs/metabolismo , Autofagia/genética , Proliferación Celular/genética , Regulación Neoplásica de la Expresión GénicaRESUMEN
By slightly vibrating the mirrors in an interferometer at different frequencies, the photons' trajectory information is stored in the light beam. To read out this information, we record the centroid location of the intensity distribution of output beam and Fourier analyze its time evolution. It is shown that every vibrating mirror contributes a peak in the Fourier spectrum. In other words, we can reveal the trajectory of the photons by figuring out the vibrating mirrors which ever interact with the light beam based on the Fourier spectrum. This techniques is not limited by the vibration amplitude of the mirrors.
RESUMEN
When a three-level atomic wavepacket is obliquely incident on a "medium slab" consisting of two far-detuned laser beams, there exists lateral shift between reflection and incident points at the surface of a "medium slab", analogous to optical Goos-Hänchen effect. We evaluate lateral shifts for reflected and transmitted waves via expansion of reflection and transmission coefficients, in contrast to the stationary phase method. Results show that lateral shifts can be either positive or negative dependent on the incident angle and the atomic internal state. Interestingly, a giant lateral shift of transmitted wave with high transmission probability is observed, which is helpful to observe such lateral shifts experimentally. Different from the two-level atomic wave case, we find that quantum interference between different atomic states plays crucial role on the transmission intensity and corresponding lateral shifts.