RESUMEN
Bioadsorption, bioaccumulation and biodegradation processes in algae, play an important role in the biomagnification of antibiotics, or other organic pollutants, in aquatic food chains. In this study, the bioadsorption, bioaccumulation and biodegradation of norfloxacin [NFX], sulfamethazine [SMZ] and roxithromycin [RTM]) is investigated using a series of culture experiments. Chlorella vulgaris was exposed to these antibiotics with incubation periods of 24, 72, 120 and 168 h. Results show the bioadsorption concentration of antibiotics in extracellular matter increases with increasing alkaline phosphatase activity (AKP/ALP). The bioaccumulation concentrations of NFX, SMZ and RTM within cells significantly increase after early exposure, and subsequently decrease. There is a significant positive antibiotics correlation to superoxide dismutase (SOD), the photosynthetic electron transport rate (ETR) and maximum fluorescence after dark adaptation (Fv/Fm), while showing a negative correlation to malondialdehyde (MDA). The biodegradation percentages (Pb) of NFX, SMZ and RTM range from 39.3 - 97.2, 41.3 - 90.5, and 9.3 - 99.9, respectively, and significantly increase with increasing Fv/Fm, density and chlorophyll-a. The accumulation of antibiotics in extracellular and intracellular substances of C. vulgaris is affected by antibiotic biodegradation processes associated with cell physiological state. The results succinctly explain relationships between algal growth during antibiotics exposure and the bioadsorption and bioaccumulation of these antibiotics in cell walls and cell matter. The findings draw an insightful understanding of the accumulation of antibiotics in algae and provide a scientific basis for the better utilization of algae treatment technology in antibiotic contaminated wastewaters. Under low dose exposures, the biomagnification of antibiotics in algae is affected by bioadsorption, bioaccumulation and biodegradation.
Asunto(s)
Chlorella vulgaris , Roxitromicina , Contaminantes Químicos del Agua , Antibacterianos/metabolismo , Chlorella vulgaris/metabolismo , Bioacumulación , Contaminantes Químicos del Agua/análisis , Biodegradación Ambiental , Norfloxacino , Roxitromicina/metabolismoRESUMEN
The ocean is an important sink for perfluorinated alkyl acids (PFAAs), but the toxic mechanisms of PFAAs to marine organisms have not been clearly studied. In this study, the growth rate, photosynthetic activity, oxidative stress and bioaccumulation were investigated using marine algae Chlorella sp. after the exposure of perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate acid (PFOS). The results showed that PFOA of <40 mg/L and PFOS of <20 mg/L stimulated algal reproduction, and high doses inhibited the algal growth. The absorbed PFOA and PFOS by algal cells damaged cell membrane and caused metabolic disorder. The photosynthesis activity was inhibited, which was revealed by the significantly reduced maximal quantum yield (Fv/Fm), relative electron transfer rate (rETR) and carbohydrate synthesis. However, the chlorophyll a content increased along with the up-regulation of its encoding genes (psbB and chlB), probably due to an overcompensation effect. The increase of ROS and antioxidant substances (SOD, CAT and GSH) indicated that PFOA and PFOS caused oxidative stress. The BCF of marine algae Chlorella sp. to PFOA and PFOS was calculated to be between 82 and 200, confirming the bioaccumulation of PFOA and PFOS in marine algae. In summary, PFOA and PFOS can accumulate in Chlorella sp. cells, disrupt photosynthesis, trigger oxidative stress and inhibit algal growth. PFOS shows higher toxicity and bioaccumulation than PFOA. The information is important to evaluate the environmental risks of PFAAs.
Asunto(s)
Ácidos Alcanesulfónicos , Chlorella , Fluorocarburos , Chlorella/metabolismo , Bioacumulación , Clorofila A , Caprilatos/toxicidad , Caprilatos/metabolismo , Fluorocarburos/toxicidad , Fluorocarburos/metabolismo , Ácidos Alcanesulfónicos/toxicidad , Ácidos Alcanesulfónicos/metabolismoRESUMEN
With the wide application as an alternative for perfluorooctane sulfonate (PFOS), perfluorobutane sulfonate (PFBS) has been frequently detected in the aquatic environment. However, the aquatic toxicity of PFBS is still poorly understood. The present work studied the aquatic toxicity of PFBS using freshwater algae Scenedesmus obliquus (S. obliquus) as indicator, and the toxicity of PFOS was also examined for comparison. The results showed that PFBS exhibited much lower toxicity to S. obliquus than PFOS. The EC50 value was higher than 1800 mg L-1 after 7 days of exposure to PFBS. By contrast, a much lower EC50 value of 136.69 mg L-1 was obtained for PFOS. Photosynthetic efficiency analyzed by chlorophyll fluorescence also verified that PFOS induced a higher toxic effect on the algae than PFBS. The malondialdehyde, catalase and superoxide dismutase results indicate that PFOS exposure led to the accumulation of ROS, which caused oxidative damage to the algae, thereby resulting in the inhibition in the growth and photosynthesis of the algae. Furthermore, transcriptome analysis indicates that the significant down-regulation of key genes related to photosynthesis induced by PFOS was the fundamental mechanism for the inhibition in photosynthetic efficiency and biomass growth of S. obliquus.
Asunto(s)
Chlorophyceae , Scenedesmus , Clorofila , Alcanosulfonatos , Estrés Oxidativo , Fotosíntesis , Perfilación de la Expresión GénicaRESUMEN
The waterborne pathogenic viruses threaten human health. And the nanomaterial-membrane coupling system is promising in virus removal. In this study, phage MS2 was selected as the model virus to investigate the removal of virus with the coupling system. Results revealed that commercial nano TiO2 (Degussa Aeroxide P25) showed both of excellent adsorption and photocatalysis performance for virus removal compared with nano ZnO, nano Fe3O4, carbon nanotube, graphene, nano Ni and Nano TiO2 (anatase). In P25 photocatalysis process, the removal efficiency of phage MS2 increased with the increase of P25 concentration (0~1000 mg L-1), virus initial concentration (102~106 PFU mL-1), UV irradiation doses (5~120 mJ cm-2) and UV light intensity (0.126~0.742 mW cm-2). However, when the P25 concentration increased to over 1000 mg L-1, the virus removal efficiency would remain stable with the increase of P25 concentration. The nanomaterial-membrane coupling system showed excellent performance for virus removal, which was mainly attributed to the adsorption and photocatalysis of P25, and the intercept of membrane. When the P25 concentration was 100 mg L-1, UV irradiation dose was 20 mJ cm-2 and transmembrane pressure was 20 kPa, the phage MS2 removal efficiency could be up to 100%.
Asunto(s)
Nanoestructuras , Titanio , Rayos Ultravioleta , Virus/aislamiento & purificación , Purificación del Agua , Adsorción , Catálisis , Grafito , LuzRESUMEN
Fe0 nanoparticles have been widely studied for pollution abatement in recent years; however, regarding the mechanism for pollutant degradation, studies have mainly focused on the reductive dechlorination by Fe0, and the dynamic process has not been clarified completely. As reported, some organics could be degraded during the oxidation of Fe0 by O2, and hydrogen peroxide was supposed to be produced. In this study, Fe3O4, an oxidation product of Fe0, was used to treat the pollutant combining with Fe0 nanoparticles, and 4-chlorophenol (4-CP) was used as the model pollutant. The results showed that the addition of Fe3O4 nanoparticles hindered the removal of 4-CP by Fe0 nanoparticles under anoxic conditions. However, the dechlorination efficiency was improved in the initial 6 h. Under aerobic conditions, the reused Fe3O4 nanoparticles would improve the removal and dechlorination of 4-CP. Especially, the dechlorination efficiency was obviously increased. It is proposed that the removal of 4-CP was due to the effects of both nanosized Fe0 and Fe3O4 - reducing action of Fe0 and catalytic oxidation action of Fe3O4. The reducing action of Fe0 was the major factor under anoxic conditions. And the catalytic oxidation action of Fe3O4 became an important reason under aerobic conditions.
Asunto(s)
Clorofenoles/química , Modelos Químicos , Nanopartículas/química , Catálisis , Halogenación , Oxidación-ReducciónRESUMEN
Nanoscale zero-valent iron (NZVI) has shown excellent performance for pathogenic microorganism removal but the inactivation mechanism has not been understood clearly enough. In this study, the bacteriophage f2 removal by NZVI under aerobic and anaerobic conditions was investigated, and various factors involved in f2 removal were analyzed in detail, including the ion products of NZVI (Fe(II), Fe(III)), solid phase products, the reactive oxygen species (ROS), O2 and H+. In addition, the morphologies of bacteriophage f2 during reaction were observed. The results showed that the removal efficiency of bacteriophage f2 was much higher under aerobic conditions than that in anaerobic systems, and oxygen and pH were determinants for f2 removal. The oxidation of Fe(II) was a fundamental step and played a significant role in bacteriophage f2 removal, especially in the aerobic systems. In the presence of oxygen, the virus removal was attributed to the generation of ROS (namely ·OH and ·O2-) and the oxidized iron, in which the ROS (·OH and ·O2-) made a predominant contribution. And the adsorption of iron oxide was responsible for the removal in oxygen depleted circumstance. In the anaerobic system, the virus removal was mainly attributed to the interaction between NZVI and bacteriophage f2. Besides, from the perspective of TEM images, the virus removal was mainly attributed to the damage of infective ability by NZVI at the initial stage of reaction, and later the virus was inactivated by the ROS generated.