The long-term release and particle fracture behaviors of nanoplastics retained in porous media: Effects of surfactants, natural organic matters, antibiotics, and bacteria.

The transport of nanoplastics (NPs) in porous media has received a lot of attention, but the studies on the long-term release of NPs retained in porous media and the particle fracture during this process are seriously lacking. For filling this deficiency, we examined the individual or synergistic effects of surfactants, natural organic matters (NOMs), antibiotics, and bacteria on the desorption, long-term release, and particle fracture behaviors of polystyrene NPs (PS-NPs) retained in porous media. It was found that the change in hydrophilicity of PS-NPs dominated the long-term release of PS-NPs retained in porous media when surfactants were present. In the single system of surfactants and the dual system of surfactants and NOMs, the release of PS-NPs were improved owing to the increasing hydrophilicity of PS-NPs, although cationic surfactants also reduced the electrostatic repulsion between PS-NPs and porous media. Increasing antibiotic concentration reduced the electrostatic repulsion between PS-NPs and porous media to inhibit the release of PS-NPs. When bacteria were present whether containing antibiotics or not, the effects on roughness of PS-NPs dominated the release of PS-NPs. The effects of surfactants and NOMs on the PS-NP desorption were similar with the long-term release, with changes in hydrophilicity dominating the process. Whereas the effects of antibiotics and bacteria on the PS-NP desorption were different with the long-term release. Surfactants and NOMs in the presence of surfactants inhibited the fracture of PS-NPs by increasing the hydrophilicity of PS-NPs brought about the coating of water molecules on PS-NPs for protection. Antibiotics had no significant effects on the fracture of PS-NPs due to unaltered vertical forces on PS-NPs and no protective effect. Bacteria in the presence or absence of antibiotics inhibited the fracture of PS-NPs by coating PS-NPs retained in porous media to protect PS-NPs from fracture.

Statuses, shortcomings, and outlooks in studying the fate of nanoplastics and engineered nanoparticles in porous media respectively and borrowable sections from engineered nanoparticles for nanoplastics.

This review discussed the research statuses, shortcomings, and outlooks for the fate of nanoplastics (NPs) and engineered nanoparticles (ENPs) in porous media and borrowable sections from ENPs for NPs. Firstly, the most important section was that we reviewed the research statuses on the fate of NPs in porous media and the main influencing factors, and explained the influencing mechanisms. Secondly, in order to give NPs a reference of research ideas and influence mechanisms, we also reviewed the research statuses on the fate of ENPs in porous media and the factors and mechanisms influencing the fate. The main mechanisms affecting the transport of ENPs were summarized (Retention or transport modes: advection, diffusion, dispersion, deposition, adsorption, blocking, ripening, and straining; Main forces and actions: Brownian motion, gravity, electrostatic forces, van der Waals forces, hydration, filtration, bridging; Affecting elements of the forces and actions: the ENP and media grain surface functional groups, size, shape, zeta potential, density, hydrophobicity, and roughness). Instead of using the findings of ENPs, thorough study on NPs was required because NPs and ENPs differed greatly. Based on the limited existing studies on the NP transport in porous media, we found that although the conclusions of ENPs could not be applied to NPs, most of the influencing mechanisms summarized from ENPs were applicable to NPs. Combining the research thoughts of ENPs, the research statuses of NPs, and some of our experiences and reflections, we reviewed the shortcomings of the current studies on the NP fate in porous media as well as the outlooks of future research. This review is very meaningful for clarifying the research statuses and influence mechanisms for the NP fate in porous media, as well as providing a great deal of inspiration for future research directions about the NP fate in porous media.

Ofloxacin weakened treatment performance of rural domestic sewage in an aerobic biofilm system by affecting biofilm resistance, bacterial community, and functional genes.

Ofloxacin (OFL) is a typical fluoroquinolone antibiotic widely detected in rural domestic sewage, however, its effects on the performance of aerobic biofilm systems during sewage treatment process remain poorly understood. We carried out an aerobic biofilm experiment to explore how the OFL with different concentrations affects the pollutant removal efficiency of rural domestic sewage. Results demonstrated that the OFL negatively affected pollutant removal in aerobic biofilm systems. High OFL levels resulted in a decrease in removal efficiency: 9.33% for chemical oxygen demand (COD), 18.57% for ammonium (NH4+-N), and 8.49% for total phosphorus (TP) after 35 days. The findings related to the chemical and biological properties of the biofilm revealed that the OFL exposure triggered oxidative stress and SOS responses, decreased the live cell number and extracellular polymeric substance content of biofilm, and altered bacterial community composition. More specifically, the relative abundance of key genera linked to COD (e.g., Rhodobacter), NH4+-N (e.g., Nitrosomonas), and TP (e.g., Dechlorimonas) removal was decreased. Such the OFL-induced decrease of these genera might result in the down-regulation of carbon degradation (amyA), ammonia oxidation (hao), and phosphorus adsorption (ppx) functional genes. The conventional pollutants (COD, NH4+-N, and TP) removal was directly affected by biofilm resistance, functional genes, and bacterial community under OFL exposure, and the bacterial community played a more dominant role based on partial least-squares path model analysis. These findings will provide valuable insights into understanding how antibiotics impact the performance of aerobic biofilm systems during rural domestic sewage treatment.

Agricultural practices and ditch size drive microbial community assembly and mediate N- and P-transformation in multistage drainage networks of paddy fields: Insights from a large-scale irrigation district in eastern China.

Agricultural drainage ditches (ADDs) are ubiquitous and regarded as active zones for biogeochemical reactions and microbe-mediated pollutant removal. However, little is known about the microbial distribution and community assembly in ADDs. Here, a typical large-scale irrigation district, including five orders of farmland drainage systems (namely field, sublateral, head, branch, and trunk ditches that could efficiently remove excess water from paddy fields to downstream water bodies), was selected to investigate the ecological processes of microbial communities and N- and P-transformation processes in multistage ditches. We found that scale effects drove distinct environmental gradients and microbial community dissimilarities and that the five ordered ditches were grouped into three clusters (field vs. sublateral vs. head, branch, and trunk ditches). Specifically, the microbial communities in the field ditches located adjacent to the paddy fields were strongly selected by agricultural fertilization and irrigation drainage, enriching salt-tolerant microbes with high nitrification and inorganic P solubilization capabilities. In comparison, the sublateral ditches showed the highest removal performance for total nitrogen (13.28-55.80%) and total phosphorus (9.06-65.07%) during the growth of rice, which was mainly attributed to the enrichment of versatile microbiota (e.g., C39, Nitrospira, and Novosphingobium) as a result of the increased stochastic processes driven by the low redox potential. Notably, the specific gene (i.e., hzsB) for anaerobic ammonium oxidation in sublateral ditches was 1-2 orders of magnitude higher than in adjacent ditches, further contributing to N loss. As field water was discharged into the large-sized head, branch, and trunk ditches, the nutrient levels decreased sharply. At the same time, deterministic processes gained more importance (∼82%), leading to the flourishing of Synechococcus and increasing the potential risk of eutrophication. Overall, the microbial communities in multistage ADDs were co-shaped by agricultural practices and ditch size, which further governed the N and P removal performance. These results provide unique insights into microbiota assembly patterns and dynamics in multistage ADDs and important ecological knowledge for controlling agricultural non-point source pollutants by managing of small-sized ditches.

A quaternary ammonium salt grafted tannin-based flocculant boosts the conjugative transfer of plasmid-born antibiotic resistance genes: The nonnegligible side of their flocculation-sterilization properties.

This study developed dual-function tannin-based flocculants, namely tannin-graft-acrylamide-diallyl dimethyl ammonium chloride (TGCC-A/TGCC-C), endowed with enhanced flocculation-sterilization properties. The impacts of these flocculants on proliferation and transformation of antibiotic resistance genes (ARGs) among bacteria during the flocculation-deposition process were examined. TGCC-A/TGCC-C exhibited remarkable flocculation capacities towards both Escherichia coli and Staphylococcus aureus, encompassing a logarithmic range of initial cell density (108-109 CFU/mL) and a broad pH spectrum (pH 2-11). The grafted quaternary ammonium salt groups played pivotal parts in flocculation through charge neutralization and bridging mechanisms, concurrently contributing to sterilization by disrupting cellular membranes. The correlation between flocculation and sterilization entails a sequential progression, where an excess of TGCC, initially employed for flocculation, is subsequently consumed for sterilization purposes. The frequencies of ARGs conjugative transfer were enhanced in bacterial flocs across all TGCC treatments, stemming from augmented bacterial aggregation and cell membrane permeability, elicited stress response, and up-regulated genes encoding plasmid transfer. These findings underscore the indispensable role of flocculation-sterilization effects in mediating the propagation of ARGs, consequently providing substantial support for the scientific evaluation of the environmental risks associated with flocculants in the context of ARGs dissemination during the treatment of raw water featuring high bacterial density.

Long-term transformation of nanoscale zero-valent iron explains its biological effects in anaerobic digestion: From ferroptosis-like death to magnetite-enhanced direct electron transfer networks.

Nanoscale zero-valent iron (nZVI) has been extensively used for environmental remediation and wastewater treatment. However, the biological effects of nZVI remain unclear, which is no doubt a result of the complexity of iron species and the dynamic succession of microbial community during nZVI aging. Here, the aging effects of nZVI on methanogenesis in anaerobic digestion (AD) were consecutively investigated, with an emphasis on deciphering the causal relationships between nZVI aging process and its biological effects. The addition of nZVI in AD led to ferroptosis-like death with hallmarks of iron-dependent lipid peroxidation and glutathione (GSH) depletion, which inhibited CH4 production during the first 12 days of exposure. With prolonged exposure time, a gradual recovery (12-21 days) and even better performance (21-27 days) in AD were observed. The recovery performance of AD was mainly attributed to nZVI-enhanced membrane rigidity via forming siderite and vivianite on the outer surface of cells, protecting anaerobes against nZVI-induced toxicity. At the end of 27-days exposure, the significantly increased amount of conductive magnetite simulated direct interspecies electron transfer among syntrophic partners, improving CH4 production. Metagenomic analysis further revealed that microbial cells gradually adapted to the aging of nZVI by upregulating functional genes related to chemotaxis, flagella, conductive pili and riboflavin biosynthesis, in which electron transfer networks likely thrived and the cooperative behaviors between consortium members were promoted. These results unveiled the significance of nZVI aging on its biological effects toward multiple microbial communities and provided fundamental insights into the long-term fates and risks of nZVI for in situ applications.

Salt tolerance evolution facilitates antibiotic resistome in soil microbiota: Evidences from dissemination evaluation, hosts identification and co-occurrence exploration.

Salinity is considered as one of the vital factors affecting the profiles of antibiotic resistance genes (ARGs) in soils, whereby its roles in shaping the antibiotic resistome were still poorly understood. Here, metagenomic analysis was conducted to track the ARGs distributions and dissemination in soils during salt accumulation and desalinization processes. Neutral-salt accumulation for 45 and 90 days significantly increased the relative abundances of ARGs and mobile genetic elements (MGEs) carrying antibiotic resistance contigs (ARCs). The ARGs within antibiotic efflux and target protection families primarily carried by Streptomyces, Nocardioides, Rhodanobacter and Monashia were largely enriched by salinity. The ARGs subtypes of the resistance-nodulation-division (RND) family, ATP-binding cassette (ABC) family, rRNA methyltransferase and other efflux were closely associated with MGEs, contributing to the enrichment of ARGs. Moreover, the ARGs subtypes and transposons were genetically linked with the salt-tolerance mechanisms of organic osmolyte transporters and K+ uptake proteins on the same ARC, demonstrating the coselection of ARGs and halotolerant genes. Furthermore, the antibiotic resistome could recover to a normal state after the prolonged incubation by alleviating salt stress. Nevertheless, the acquisition of ARGs by opportunistic pathogens after salt treatment was increased, serving to prioritize further efforts on the health risks correlated with resistance propagation and human exposure in saline soils.

Influence of aggregation and sedimentation behavior of bare and modified zero-valent-iron nanoparticles on the Cr(VI) removal under various groundwater chemistry conditions.

Aggregation behaviors of bare, and sodium polyacrylate (PAA) and starch modified zero-valent-iron nanoparticles (nZVI), as well as their effects on the Cr (VI) removal were investigated by simulating the groundwater. Results showed that increased concentration of PAA (1-6 wt%) and starch (0.1-0.6 wt%) alleviated the aggregation of modified nZVI (abbreviated as P-nZVI and S-nZVI), while there was an optimum dosage of 4 wt% PAA and 0.3 wt% starch for the Cr (VI) removal, respectively. Moreover, as one of the fundamental water chemistry parameters, Ca2+ (0, 5, and 10 mg L-1) greatly promoted the aggregation of modified nZVI, and decreased the Cr (VI) removal efficiency by them via forming bidentate bridging structure (between Ca2+ and PAA) or complexes (between Ca2+ and starch). Additionally, fulvic acid (FA) (0, 2, 5, and 10 mg L-1) decreased the Cr (VI) removal by P-nZVI because of the significantly improved electronic repulsion. However, FA enhanced the aggregation of S-nZVI, but diminished its performance on Cr (VI) removal due to the bridging effect between FA and starch. The present study was of great importance in predicting the migration of nZVI and contaminants removal under complex geological conditions in groundwater.

Antibiotic resistance genes alternation in soils modified with neutral and alkaline salts: interplay of salinity stress and response strategies of microbes.

Growing evidence points to the pivotal roles of salt accumulation in mediating antibiotic resistance genes (ARGs) spread in soil, whereas how salt mediates ARGs dissemination remains unknown. Herein, the effects of neutral or alkaline (Ne/Al) salt at low, moderate and high levels (Ne/Al-L, Ne/Al-M, Ne/Al-H) on the dissemination of ten typical ARGs in soils were explored, by simultaneously considering the roles of salinity stress and response strategies of microbes. In the soils amended with Ne/Al-L and Al-M salt, the dissemination of ARGs was negligible and the relative abundances of ARGs and mobile genetic elements (MGEs) were decreased. However, Ne-M and Al-H salt contributed to the dissemination of ARGs in soils, with the significantly increased absolute and relative abundances of ARGs and MGEs. In Ne-H soil, although the absolute abundance of ARGs declined drastically due to serious oxidative damage, their relative abundances were promoted. The facilitated ARGs transfer was potentially related to the excessive generation of intracellular reactive oxygen species and increased activities of DNA repair enzymes involved in SOS system. In addition, the activated intracellular protective response including quorum sensing and energy metabolism largely provided essential factors for ARGs dissemination. The co-occurrence of ARGs and over-expressed salt-tolerant genes in specific halotolerant bacteria further suggested the selection of salt stress on ARGs. Moreover, less disturbance of alkaline salt than neutral salt on ARGs evolution was observed, due to the lower abiotic stress and selective pressure on microbes. This study highlights that soil salinity-sodicity could dose-dependently reshape the dissemination of ARGs and community structure of microbes, which may increase the ecological risks of ARGs in agricultural environment.

Intimately coupled photocatalysis and biodegradation for effective simultaneous removal of sulfamethoxazole and COD from synthetic domestic wastewater.

The inefficiency of conventional biological treatment for removing sulfamethoxazole (SMX) is posing potential risks to ecological environments. In this study, an intimately coupled photocatalysis and biodegradation (ICPB) system consisting of Fe3+/g-C3N4 and biofilm was fabricated for the treatment of synthetic domestic wastewater containing SMX. The results showed that this ICPB system could simultaneously remove 96.27 ± 5.27% of SMX and 86.57 ± 3.06% of COD, which was superior to sole photocatalysis (SMX 100%, COD 4.2 ± 0.74%) and sole biodegradation (SMX 42.21 ± 0.86%, COD 95.1 ± 0.18%). Contributors to SMX removal in the ICPB system from big to small include LED photocatalysis, biodegradation, LED photolysis, and adsorption effect of the carrier, while COD removal was largely ascribed to biodegradation. Increasing initial SMX concentration inhibits SMX removal rate, while increasing photocatalyst dosage accelerates SMX removal rate, and both had no impact on COD removal. Our analysis of biofilm activity showed that microorganisms in this ICPB system maintained a high survival rate and metabolic activity, and the microbial community structure of the biofilm remained stable, with Nakamurella and Raoultella being the two dominant genera of the biofilm. This work provides a new strategy to effectively treat domestic wastewater polluted by antibiotics.

Attenuation effects of iron on dissemination of antibiotic resistance genes in anaerobic bioreactor: Evolution of quorum sensing, quorum quenching and dynamics of community composition.

Zero valent iron (ZVI) coupled with bioreactors is arising as a promising technology for antibiotic resistance genes (ARGs) mitigation, whereas the succession and behaviors of microbes caused by ZVI in relieving ARGs propagation remain unclear. Herein, the effects of ZVI on microbial quorum sensing (QS), quorum quenching (QQ) system and community dynamics were examined in anaerobic bioreactor fed with oxytetracycline (tet), to illustrate the roles of evolutive microbial communication and community composition in ARGs attenuation. With the addition of 5 g/L ZVI, the total absolute abundance of tet ARGs was retarded by approximate 95% and 72% in sludge and effluent after 25 days operation. The abundance of mobile genetic elements and the heredity of antibiotic resistant bacteria revealed the declined horizontal and vertical transfer of ARGs, which directly led to the reduced ARGs propagation. Potential mechanisms are that the positive effects of ZVI on QQ activity via the functional bacteria enrichment inhibited QS system and thus ARGs transfer. Partial least--squares path modeling further demonstrated that ARGs abundance was strongly limited by the dynamics of bacterial composition and thereby less frequent microbial communication. These results provide new insights into the mechanisms of antibiotic resistome remission in anaerobic bioreactor modified by ZVI.

Biofilm influenced metal accumulation onto plastic debris in different freshwaters.

Microbial biofilms can rapidly colonize plastic debris in aquatic environments and subsequently, accumulate chemical pollutants from the surrounding water. Here, we studied the microbial colonization of different plastics, including polyethylene terephthalate (PET), polypropylene (PP), polyvinyl chloride (PVC), and polyethylene (PE) exposed in three freshwater systems (the Qinhuai River, the Niushoushan River, and Donghu Lake) for 44 days. We also assessed the biofilm mass and associated metals attached to plastics. The plastics debris characteristics, such as contact angle and surface roughness, greatly affected the increased biofilm biomass. All types of metal accumulation onto the plastic substrate abundances significantly higher than the concentrations of heavy metal in the water column, such as Ba (267.75 µg/g vs. 42.12 µg/L, Donhu Lake), Zn (254 µg/g vs. 0.023 µg/L the Qinhuai River), and Cr (93.75 µg/g vs. 0.039 µg/L, the Niushoushan River). Compared with other metals, the heavy metal Ba, Cr and Zn accumulated easily on the plastic debris (PET, PP, PVC, and PE) at all incubation sites. Aquatic environmental factors (total nitrogen, total phosphorus, and suspended solids concentrations) largely shaped metal accumulation onto plastic debris compared with plastic debris properties.

Antibiotic resistance genes attenuation in anaerobic microorganisms during iron uptake from zero valent iron: An iron-dependent form of homeostasis and roles as regulators.

Zero valent iron (ZVI) has been previously documented to attenuate the propagation of antibiotic resistance genes (ARGs) in microbes, while how ZVI affects the evolution of ARGs remains unclear. Herein, we investigated the influences of ZVI on ARGs dissemination in anaerobic bioreactor treating oxytetracycline (tet) containing wastewater, by deciphering the roles of iron homeostasis and regulatory effects. A net reduction of tet gene targets ranging from 0.75 to 1.88 and 0.67 to 2.08 log unit in intracellular and extracellular DNA was achieved at the optimal dosage of 5 g/L ZVI, whereas 20 g/L ZVI made no effects on ARGs reduction. The reduced ARGs abundance by ZVI was directly related to the inhibited horizontal transfer of ARGs and decreased proliferation of resistant strains (mainly Paludibacter and WCHB1-32). The potential mechanisms included the increased antioxidant capacity, the depressed efflux pump system and the weakened energy driving force by Fur regulon in microbes (especially for Cloacibacterium and Dechloromonas). The negligible influence of 20 g/L ZVI on ARGs reduction was ascribed to the iron-catalyzed oxidative damage and reduced physiological activity. This study firstly illustrated the potential relationships among activation of iron uptake regulator leading to protection against oxidative stress, alternation of physiological metabolisms and reduction of ARGs dissemination. This work extents our understanding about the priority of ZVI in mitigating ARGs proliferation and sheds light on its potential application in wastewater treatment plants.

Deciphering the effects of CeO2 nanoparticles on Escherichia coli in the presence of ferrous and sulfide ions: Physicochemical transformation-induced toxicity and detoxification mechanisms.

The physicochemical transformations as well as the redox reaction-induced toxicity changes of ceria nanoparticles (CeO2 NPs) in reducing conditions is extremely lacking. Herein, the behaviors, chemical modifications and toxicity of CeO2 NPs in the presence of reduction-active ions (namely Fe2+ and S2-) were investigated, with a particular emphasis on the cytotoxicity mechanism associated with their physicochemical transformations. The presence of Fe2+ and S2- differently altered the surface properties and toxicity of CeO2 NPs. Redox reactions with Fe2+ led to form small aggregates, boosted the reduction of CeIVO2 and enhanced dissolved Ce3+ concentration. Moreover, CeO2 NPs possessed a high affinity for Escherichia coli (E. coli) and induced the generation of •OH abiotically after reaction with Fe2+, provoking serious disruption of cell membranes and causing high toxicity to E. coli. In contrast, the amending of S2- protected E. coli from direct contact with CeO2 NPs by creating new Ce2S3 precipitated on the surface, accelerating the aggregation of NPs and reducing the concentration of dissolved Ce3+. This study suggested that the chemical interactions between the reactive surfaces of CeO2 and reduction-active ions highly determined the stability and cytotoxicity of CeO2 NPs, which provides fundamental insights into the environmental risks of CeO2 NPs.

Distinct microbial metabolic activities of biofilms colonizing microplastics in three freshwater ecosystems.

Concerns are growing about the increasing amounts of microplastics (MPs) and their ecological impacts, especially the influences of "plastisphere" in the freshwater ecosystems. Although the microbial structure and composition of biofilms are investigated, knowledge of their microbial functions remains limited. Herein, we investigated the functional diversity of carbon metabolism in biofilms colonizing one inert (glass) and two MPs as polyvinyl chloride (PVC) and polyethylene terephthalate (PET) substrates incubated for 44 days in situ in the Niushoushan River, the Qinhuai River, and Donghu Lake. 2D confocal laser scanning microscopy images visualized distinct micro-structures and biofilm compositions on three substrates. BIOLOG ECO microplates indicated variation on carbon utilization capacities of biofilms of inert and MPs in three freshwater ecosystems. Biofilms on PET showed lower capacities and carbon metabolism rates than those on glass and PVC, indicating the presence of substrate-specific functional diversity. The Shannon-Wiener diversity, Simpson diversity and Shannon evenness indices for the Niushoushan River and Donghu Lake were ordered as glass > PVC > PET. Besides to MPs-specific factors, environmental factors including nutrient (i.e., TN and TP) and turbidity largely shaped biofilm carbon metabolism. Overall findings demonstrated that as specific niches, MPs influenced microbial-mediated carbon cycling in the freshwater ecosystems and MPs-promoted microbial communities posed ecological significance.

Surface Properties and Environmental Transformations Controlling the Bioaccumulation and Toxicity of Cerium Oxide Nanoparticles: A Critical Review.

Increasing production and utilization of cerium oxide nanoparticles (CNPs) in recent years have raised wide concerns about their toxicity. Numerous studies have been conducted to reveal the toxicity of CNPs, but the results are sometimes contradictory. In this review, the most important factors in mediating CNPs toxicity are discussed, including (1) the roles of physicochemical properties (size, morphology, agglomeration condition, surface charge, coating and surface valence state) on CNPs toxicity; (2) the phase transfer and transformation process of CNPs in various aqueous, terrestrial, and airborne environments; and (3) reductive dissolution of CNPs core and their chemical reactions with phosphate, sulfate/S2-, and ferrous ions. The physicochemical properties play key roles in the interactions of CNPs with organisms and consequently their environmental transformations, reactivity and toxicity assessment. Also, the speciation transformations of CNPs caused by reactions with (in)organic ligands in both environmental and biological systems would further alter their fate, transport, and toxicity potential. Thus, the toxicity mechanisms are proposed based on the physical damage of direct adsorption of CNPs onto the cell membrane and chemical inhibition (including oxidative stress and interaction of CNPs with biomacromolecules). Finally, the current knowledge gaps and further research needs in identifying the toxicological risk factors of CNPs under realistic environmental conditions are highlighted, which might improve predictions about their potential environmental influences. This review aims to provide new insights into cost-effectiveness of control options and management practices to prevent environmental risks from CNPs exposure.

Microbial carbon metabolic functions of biofilms on plastic debris influenced by the substrate types and environmental factors.

As an artificial type of microbial carrier, plastic debris has been widely detected in freshwater habitats, and the potential impacts of the plastisphere (biofilms colonized on plastics) in aquatic ecosystems have drawn increasing attention. Distinct community compositions and structures of biofilms in plastic and natural substrates have been recorded in freshwater environments. However, the microbial metabolic functioning of the plastisphere was underestimated, especially in freshwater environments. In this study, the effects of substrate types on the carbon metabolic functions of biofilms were studied by in situ cultivation of biofilms on plastics (polyvinyl chloride, PVC and polyethylene, PE) and natural substrate (cobblestone) for 44 days in two rivers (the Niushoushan River and the Qinhuai River) and two lakes (Donghu Lake and Xuanwu Lake). Biofilms on plastics showed higher biomasses than those on natural substrates in all ecosystems. Variations in the micro-structure and compactness of biofilms developed under different substrates were observed from scanning electron microscope and confocal laser scanning microscope image analyses. The carbon metabolic activities of the biofilms evaluated by BIOLOG EcoPlate were different between plastics (PVC and PE) and natural substrate (cobblestone) in the four freshwater ecosystems. In the Niushoushan River, PE-associated biofilms had different capacity in using carbon sources from cobblestone-associated biofilms as illustrated by the Shannon-Wiener diversity index and Shannon evenness index. Additionally, the metabolic functional diversity profiles of biofilms on PVC were significantly different from those on cobblestone in the other three aquatic ecosystems. Moreover, results from variation partitioning analysis suggested that the impact of environmental factors (contribution: 21%) on microbial carbon metabolic functions was much greater than that of substrate types (contribution: 6%). These findings illustrated distinct microbial functions of biofilms inhabited on plastics, and environmental factors play a decisive role in the differentiation and specificity of carbon metabolism of the plastisphere. This study offers new insights that plastics serving as artificial microbial niches have the ability to affect the microbial-mediated carbon cycling process in aquatic ecosystems.

Insights into spatial effects of ceria nanoparticles on oxygen mass transfer in wastewater biofilms: Interfacial microstructure, in-situ microbial activity and metabolism regulation mechanism.

Growing international exploitation of ceria nanoparticles (CeO2 NPs) for varied applications has increased their release into wastewater treatment plants. Mass transfer of oxygen (MTO) in wastewater biofilm is of considerable importance to influence the activity and purification ability of biofilm. Herein, we investigated the spatial distribution of oxygen in gas-liquid-biofilm phases, the microstructure of interfaces and the in-situ microbial activity to reveal the impacts of CeO2 NPs on MTO in wastewater biofilm and the related mechanisms. After exposure to 1 and 10 mg/L CeO2 NPs, the oxygen transfer coefficient (KLa) from gas to wastewater increased by 28.1% and 75.3% with a reduction of thickness in gas-liquid boundary layer, indicating the enhanced MTO in gas-liquid interface. In contrast, the MTO in liquid-biofilm interface was negatively affected and the thickness of liquid-biofilms boundary layer significantly increased, which was mainly attributed to the smoother surface and the decreased surface area difference of biofilm. Within biofilm, the microbial activity was inhibited by 10 mg/L CeO2 NPs, whereas the production of extracellular polymeric substance (EPS) was significantly improved, leading to a decline of 35.0% in the internal effective diffusivity (DB) and a 300-µm reduction of oxygen penetration depth. Moreover, the relative activities of key enzymes involved in glycometabolism indicated the transition of Embden-Meyerhof pathway to pentose phosphate pathway, which probably contributed to the enhanced EPS production and consequently increased mass transfer resistance in liquid-biofilm interface and inner biofilm. These results could potentially expand the knowledge on mass transfer of nutrients or pollutants in wastewater biofilm in response to NPs exposure.

Transport behavior of micro polyethylene particles in saturated quartz sand: Impacts of input concentration and physicochemical factors.

The long-term contamination of soil by microplastics may pose risks that are often still not well understood, and the ecological effects of microplastics are mainly dependent on their environmental behavior in environments. This study used saturated quartz sand as a solid porous medium to study the migration and influencing factors of 40-48 µm polyethylene (PE) particles in saturated porous media. The breakthrough curves at different injection concentrations (0.3, 0.4, 0.5 mg/L), flow rates (1.0, 1.5, 2.0, 2.5 ml/L), porous medium particle sizes (1-2, 2-4 mm), ionic strengths (0, 0.01, 0.05 mol/L) and concentrations of fulvic acid (FA) (0, 5, 10 mg/L) were compared and analyzed. The Derjaguin-Landau-Verwey-Overbeek (DLVO) theory was used to more accurately explain relevant transport behaviors. The results showed that the input concentration, flow rate, and particle size can affect the migration of PE particles individually or in combination. As ionic strength increased, the repulsion between microplastics and quartz sand gradually disappeared according to DLVO theory, and their attraction gradually strengthened. As a result, fewer microplastics could penetrate the sand column and reach the water body. With the continuous addition of FA, the repulsive energy between microplastics and quartz sand rose from DLVO theory, and the migration ability of microplastics initially increased before becoming stable because of the effect of straining. In all cases, the migration ability of PE was low (C/C0 < 0.35), and most PE particles remained in the porous media during the whole experimental periods. This study provides new insights of understanding the migration of microplastics in environment.

Effects of Nanoplastics on Freshwater Biofilm Microbial Metabolic Functions as Determined by BIOLOG ECO Microplates.

Nanoplastic (NP) contamination is becoming a pervasive issue as NPs, originating from microplastic particles, pose potentially harmful environmental impacts on aquatic ecosystems. The environmental hazards of NPs on microorganisms have been well documented in recent studies; however, little is known about their ecotoxicity effects on freshwater biofilms, which serve as important primary producers and decomposers and are highly connected with other ecosystem components. We investigated the effects of NPs on the microbial metabolic functions of freshwater biofilms in terms of carbon source utilization ability. Biofilm samples were collected, cultivated in a hydrodynamic flume for six weeks, and then exposed in polystyrene (PS) beads (100 nm in size) with different NP concentrations (1, 5, and 10 mg/L). BIOLOG ECO microplates were used to quantify carbon source utilization characteristics. The data were analyzed using average well-color development (AWCD), functional diversity indices, and principle component analysis (PCA). Results showed that the total carbon metabolic functions (represented by AWCD) remained constant (p > 0.05) with elevated NP concentrations, but some specific carbon sources (e.g., esters) changed in their utilization ability (p < 0.05). The microbial functional diversity (Shannon-Wiener diversity index, Simpson diversity index, and Shannon evenness index) was significantly reduced under 10 mg/L NPs (p < 0.05), indicating an inhibiting effect of NPs on biofilm metabolic diversity. This study examined NP ecotoxicity effects on microbial metabolic activities at the community level, but further studies are required to fully understand the mechanisms driving this change.