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[This corrects the article DOI: 10.3389/fbioe.2024.1354241.].
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Objective: The present study aimed to assess the bond strength and durability of six bonding agents concerning their application to metal or ceramic brackets and zirconia. Materials and Methods: Six resin cement bonding agents (XT, XTS, RSBU, RGBU, SBPM, and GMP) were chosen for this investigation. Specimens were either stored in distilled water at 37°C for 24 h or subjected to 5,000 thermocycles before conducting a Shear Bond Strength (SBS) test. Statistical analysis of the SBS data was performed using three-way ANOVA and Games-Howell tests (α = 0.05). The Adhesive Remnant Index was examined, and the debonding surface details on brackets and zirconia were observed. Results: For metal brackets, all groups demonstrated clinically acceptable bond strength, irrespective of storage conditions, except for the XT group. Regarding ceramic brackets, all groups displayed acceptable bond strength after 24 h of water storage. However, following thermocycling, a significant decrease in SBS was noted across all groups (p < 0.05), with SBPM exhibiting a higher bond strength. Three-way ANOVA analysis indicated that SBS values were notably influenced by each factor, and an interaction among the three independent variables was observed (p = 0.000). Conclusion: The reliable bond strength between ceramic brackets and zirconia was significantly lower after thermocycling compared to that of metal brackets and zirconia. SBPM exhibited consistent and robust bond strength between ceramic/metal brackets and zirconia across various storage conditions. Furthermore, the HEMA-free adhesive demonstrated a potentially more consistent bonding performance compared to the HEMA-containing adhesive employed in this study.
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Porphyromonas gingivalis (P. gingivalis) is the most common species that causes peri-implantitis. It forms an irreversible dense biofilm and causes inflammation. A novel 3D-printed porous TC4-6Cu alloy was fabricated using selective laser melting (SLM) technology for the dental implant, which is anticipated to inhibit biofilm formation. We attempted to investigate the antibacterial ability and antibacterial mechanism of the 3D-printed porous TC4-6Cu alloy against P. gingivalis. This work used scanning electron microscopy (SEM) and laser confocal microscopy (CLSM) to detect the antimicrobial ability of the alloy against sessile P. gingivalis. The results indicated that the 3D-printed porous TC4-6Cu alloy could cause bacterial fragmentation and deformation. Plate antimicrobial counting experiments showed that the antibacterial rates of the alloy against adherent bacteria and planktonic bacteria after 24 h were 98.05% and 73.92%, respectively. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of Cu2+ were tested to appraise the antibacterial property of the alloy against planktonic P. gingivalis. The relationship between the antibacterial mechanism of the alloy with oxidative stress was evaluated through ROS fluorescence intensity and protein leakage concentration. The results revealed that the alloy significantly eliminated adherent bacteria and inhibited biofilm formation. Moreover, 3D-printed porous TC4-6Cu alloy demonstrated significant bactericidal ability by inducing the production of reactive oxygen species (ROS), which could result in protein leakage from the bacterial cell membrane. This research may open a new perspective on the development and biomedical applications for dental implantation.
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OBJECTIVES: This study aims to evaluate the color stability and related properties including water sorption and solubility of ten light-cured composite resins in different solutions. METHODS: A total of 10 composite resins were Beautifilâ ¡(B2) and Ceram. X One Universal (CXU), Charisma (CS), Charisma Diamond (CD), Denfil (DF), DX. Universal (DXU), Filtek Z250 (Z250), Filtek Z350 XT (Z350), FS-1 (FS), and Magnafill Putty (MP). Meanwhile, a total of 20 disk-shaped samples were fabricated and randomly divided into four groups (n=5), which were immersed in distilled water (control group), curry, coffee, and red wine for 28 days. The color (CIE L∗a∗b∗) was measured by a spectrophotometer at baseline and 1, 7, 14, 21, and 28 days after immersion, and the color differences were calculated. Water sorption and solubility values were measured ba-sed on ISO 4049: 2019. In addition, three-way ANOVA was used to evaluate the influence of resin materials, solutions, and immersion time on discoloration results, meanwhile, one-way ANOVA was used to compare the water sorption values and solubility values of different materials. RESULTS: All samples showed a certain degree of color change with time. Color differences were significantly influenced by materials, solutions, and immersion time (P<0.001). The color changes of the measured materials at any time point: curry>red wine>coffee>distilled water. Thus, all materials showed clinically unacceptable discoloration (ΔE>3.3) after immersing in staining curry, coffee, and red wine for 7 days. Therefore, when immersed in curry for 28 days, CS and DXU had the smallest and the largest color difference. In addition, when immersed in coffee for 28 days, FS showed the smallest color change and DXU showed the largest. Moreover, when immersed in red wine for 28 days, FS showed the smallest color change and Z350 showed the largest. Furthermore, MP and CXU had small color differences in all solutions. Meanwhile, Z350 had the highest water sorption and MP had the lowest. The solubility values of CS and CD were significantly higher than those of other materials. CONCLUSIONS: The color stability of light-cured composite resin is materials-depended and affected by pigment types and immersion time. Thus, MP and CXU have better color stability. MP has low water sorption.
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Café , Agua , Color , Resinas Compuestas , Materiales Dentales , Ensayo de Materiales , Solubilidad , Espectrofotometría , Propiedades de SuperficieRESUMEN
This study aims to assess the color stability, water sorption, and solubility of 11 resin composites as commercially available dental products. Twenty samples (10 mm in diameter and 2 mm in thickness) of each material were fabricated using a customized silicone mold, followed by immersion in each of curry, coffee, wine, and distilled water for 28 days (n = 5). Baseline shade and color changes (ΔE) were measured using a reflection spectrophotometer. The CIE L*, a*, b* system was used to evaluate the color changes. Five samples of each resin composite were applied to test water sorption and solubility according to ISO 4049:2009. As a result, the ∆E values were significantly influenced by each of the three factors (composition of material, solution, time) and the interactions between them (p < 0.001). Highest resistance to discoloration was achieved by Ceram.X One Universal (CXU), followed by Magnafill Putty (MP). Generally, microhybrid composites showed fewer color changes than nanohybrid composites and giomers. DX. Universal and Filtek Z350 XT showed the highest ΔE values in all colorants. All materials tested in this study fulfilled the criteria of ISO 4049:2009; CXU and MP had the lowest water sorption and solubility. The Pearson test showed statistically significant positive correlations between water sorption and ΔE and between solubility and ΔE.
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This study aimed to evaluate the shear bond strength (SBS) of four bonding agents used to bond metal brackets to zirconia under different storage conditions. Four bonding agents were used [FLC: (Fuji ORTHO LC), XT: (TransbondTM XT), RUC-SBU: (Rely XTM Ultimate Clicker Adhesive Resin Cement+Single Bond Universal), and RUC-GBU: (Rely XTM Ultimate Clicker Adhesive Resin Cement+Gluma Bond Universal)] to bond two types of metal brackets (PT/3M) to zirconia surfaces, and they were stored in water at 37ºC for 24 h or thermocycling for 3,000 cycles. The SBS data of RUC-SBU and RUC-GBU using PT brackets were significantly higher than those of 3M brackets before and after thermocycling. It could be concluded that RUC-SBU and RUC-GBU could offer sufficient bond strength between metal brackets and zirconia for the short term compared with FLC and XT. The design of brackets can significantly affect the bond strength to zirconia.
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Recubrimiento Dental Adhesivo , Soportes Ortodóncicos , Análisis del Estrés Dental , Ensayo de Materiales , Cementos de Resina/química , Resistencia al Corte , Agua/química , CirconioRESUMEN
Orthopedic hybrid implants combining both titanium (Ti) and magnesium (Mg) have gained wide attraction nowadays. However, it still remains a huge challenge in the fabrication of Mg-Ti composites because of the different temperatures of Ti melting point and pure Mg volatilization point. In this study, we successfully fabricated a new Mg-Ti composite with bi-continuous interpenetrating phase architecture by infiltrating Mg melt into Ti scaffolds, which were prepared by 3D printing and subsequent acid treatment. We attempted to understand the 7-day degradation process of the Mg-Ti composite and examine the different Mg2+ concentration composite impacts on the MC3T3-E1 cells, including toxicity, morphology, apoptosis, and osteogenic activity. CCK-8 results indicated cytotoxicity and absence of the Mg-Ti composite during 7-day degradation. Moreover, the composite significantly improved the morphology, reduced the apoptosis rate, and enhanced the osteogenic activity of MC3T3-E1 cells. The favorable impacts might be attributed to the appropriate Mg2+ concentration of the extracts. The results on varying Mg2+ concentration tests indicated that Mg2+ showed no cell adverse effect under 10-mM concentration. The 8-mM group exhibited the best cell morphology, minimum apoptosis rate, and maximum osteogenic activity. This work may open a new perspective on the development and biomedical applications for Mg-Ti composites.
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OBJECTIVE: To assess the clinical status of monolithic zirconia CAD/CAM endocrowns and crowns on post and core buildups in endodontically treated molars after 5 years of clinical service. MATERIALS AND METHODS: A total of 670 patients who received restorations for excessive defects at Ansteel Group Hospital from 2012 to 2015 were selected and randomly divided into two groups: 334 patients in the endocrown group and 336 in the post and core crown group. All the treatments were performed using a digital CAD/CAM system. The prostheses restorations at 1, 3, and 5 years after treatment were evaluated using the USPHS (United States Public Health Service) criteria and satisfaction questionnaire. In addition, the time cost of tooth preparation was recorded by the clinicians. RESULTS: Most of the patients in both groups reported grade A at the after-treatment follow-ups. Gingival health, food impaction, marginal accuracy, and general satisfaction at 3 and 5 years after treatment in the endocrown group rated higher (P < 0.05) than in the post and core crown group. There was no statistical difference in other indicators between the two groups at all observational after-treatment timepoints (P > 0.05). The time cost of tooth preparation in the post and core crown group was significantly higher than that in the endocrown group. CONCLUSION: A CAD/CAM scanning system combined with a monolithic zirconia endocrown restoration was rated very positively by the participants. Endocrown restorations are a significantly superior choice compared with post and core crowns in clinical application. (Int J Comput Dent 2022;25(3):287-294; doi: 10.3290/j.ijcd.b2599661).
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Coronas , Diseño de Prótesis Dental , Cerámica , Diseño Asistido por Computadora , Porcelana Dental , Humanos , Ensayo de Materiales , CirconioRESUMEN
The purpose of this study was to evaluate the air-blowing temperature and water storage time on the micro-tensile bond strength (µTBS) of five universal adhesive systems to dentin. The bond strength with two different air-blowing temperatures (60±2ºC and 23±2ºC) was measured after water storage at 37ºC for 24 h and 100 days respectively. The fracture surface on dentin side was observed by scanning electron microscope (SEM). Three-way ANOVA revealed a significant effect of universal system (p<0.001) and air-blowing temperature (p<0.001) on bond strength to dentin except water-storage time (p=0.145). The interaction within three factors was significantly different (p<0.001). It could be concluded that the µTBS of universal systems to dentin was material-depended. The higher and more stable bonding performance of universal systems on dentin could be achieved by air-blowing at 60±2ºC temperature. In addition, the quantity of voids in the adhesive layer of aceton-based universal adhesive was significantly reduced by higher temperature.
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Recubrimiento Dental Adhesivo , Recubrimientos Dentinarios , Cementos Dentales , Dentina , Ensayo de Materiales , Cementos de Resina , Temperatura , Resistencia a la Tracción , AguaRESUMEN
cmesenchymalepithelial transition (Met) is a transmembrane tyrosine kinase receptor of hepatocyte growth factor (HGF). HGF/Met signaling stimulates numerous pathways, including the Ras/mitogenactivated protein kinase (MAPK), phosphatidylinositol 3kinase/protein kinase B and Wnt/ßcatenin pathways, which serve important roles in cell proliferation, survival, motility, invasion and angiogenesis, and promotes the development and progression of tumors. Aberrant HGF/Met signaling is associated with a poor prognosis in several types of tumors, including head and neck squamous cell carcinoma (HNSCC). Although, the HGF/MET pathway and HGF and/or Met inhibitors have been extensively reviewed, their role in tumor immunity remains elusive. The present review article summarizes the findings on the HGF/Met signaling in HNSCC, including gene and protein alterations, biological functions and patient outcomes. Furthermore, the role of HGF/Met in tumor immunity is discussed and the controversial association between the expression of HGF/Met and the prognosis of patients with HNSCC from the perspective of tumor immunity is clarified. Ultimately, the present review proposes a clinical approach that may improve the efficacy of Met therapy for HNSCC, namely the intratumoral administration of Met inhibitors in order to reduce the inhibitory effect on immune cell recruitment. However, further studies are required to provide an improved understanding of the effects of the HGF/Met pathway on the tumor microenvironment, and the effects of HGF and Met inhibitors on immune cells in the tumor environment should be the focus of future studies.
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Neoplasias de Cabeza y Cuello/inmunología , Factor de Crecimiento de Hepatocito/metabolismo , Proteínas Proto-Oncogénicas c-met/metabolismo , Transducción de Señal/inmunología , Carcinoma de Células Escamosas de Cabeza y Cuello/inmunología , Antineoplásicos/farmacología , Antineoplásicos/uso terapéutico , Línea Celular Tumoral , Regulación Neoplásica de la Expresión Génica/inmunología , Neoplasias de Cabeza y Cuello/genética , Neoplasias de Cabeza y Cuello/mortalidad , Neoplasias de Cabeza y Cuello/patología , Factor de Crecimiento de Hepatocito/antagonistas & inhibidores , Factor de Crecimiento de Hepatocito/genética , Humanos , Inyecciones Intralesiones , Pronóstico , Proteínas Proto-Oncogénicas c-met/antagonistas & inhibidores , Proteínas Proto-Oncogénicas c-met/genética , Transducción de Señal/efectos de los fármacos , Transducción de Señal/genética , Carcinoma de Células Escamosas de Cabeza y Cuello/genética , Carcinoma de Células Escamosas de Cabeza y Cuello/mortalidad , Carcinoma de Células Escamosas de Cabeza y Cuello/patología , Microambiente Tumoral/efectos de los fármacos , Microambiente Tumoral/genética , Microambiente Tumoral/inmunologíaRESUMEN
OBJECTIVE: This study aimed to compare the effects of water storage treatment and thermal cycling on the shear bond strength (SBS) of three self-adhesive dual-cure resin cements. METHODS: Six cubic zirconia specimens with side length of 2 cm were obtained by cutting and sintering. Three self-adhering dual-cure resin cements (i.e., Clearfil SAC, RelyX U200, and Multilink Speed) were selected. According to their bonding modes, they were divided into three groups: direct bonding group (direct coating with resin cement), adhesive group (applying universal adhesives and then coating with resin cement), and primer group (applying Z-Prime Plus and then coating with resin cement). According to experimental conditions, each group was divided into two subgroups: subgroup a (water storage at 37 â for 24 h) and subgroup b (thermalcycling for 5 000 times). SBS data were analyzed by one-way ANOVA by using SPSS 19.0 software (P<0.05). The fractured zirconia surface was observed under a stereomicroscope. RESULTS: After water storage for 24 h, the SBS of the adhesive group and the primer group of the three resin cements was higher than that of the direct adhesive group (P<0.05), but the difference in SBS between the adhesive group and the primer group was not significant (P>0.05). After thermalcycling, the SBS of the three types of resin cements decreased (P<0.05); the SBS of the adhesive group was higher than that of the direct adhesive group and the primer group (P<0.05). Fracture mode analysis revealed that the type â ¢ fracture mode evidently increased after the thermalcycling treatment compared with the water storage treatment. CONCLUSIONS: The universal adhesives and the primer can improve the SBS of self-adhesive dual-cure resin cement in water storage at 37 â for 24 h. The universal adhesives had a better bonding durability than the zirconia primer.
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Recubrimiento Dental Adhesivo , Cementos de Resina , Cerámica , Cementos Dentales , Análisis del Estrés Dental , Ensayo de Materiales , Resistencia al Corte , Propiedades de Superficie , CirconioRESUMEN
OBJECTIVE: This study aimed to determine the effects of copper content on the corrosion resistance of CoCrMoCu alloy and its in vitro antibacterial performance. METHODS: CoCrMoCu specimens with different Cu contents (2%, 3%, 5%, and 7%) were prepared by vacuum melting method. CoCrMo without Cu served as control. The corrosion resistance of the specimens was measured by electrochemistry. The antibacterial effects of the specimens on Staphylococcus aureus and Escherichia coli were analyzed by coating-film method. RESULTS: Compared with CoCrMo without Cu, the addition of 2%-5% Cu to CoCrMo improved the pitting and uniform corrosion of CoCrMo alloy and decreased the corrosion current density. The antibacterial performance of CoCrMoCu alloy increased with increased Cu content. The antibacterial rate of alloy was 99% when Cu content exceeded 5%. CONCLUSIONS: Cu addition had a statistically significant influence on the corrosion resistance and antibacterial performance of CoCrMoCu. CoCrMoCu has better corrosion resistance and antibacterial performance when Cu content is 5%.
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Antibacterianos , Cobre , Aleaciones Dentales , Antibacterianos/farmacología , Cobre/farmacología , Corrosión , Escherichia coli/efectos de los fármacos , Staphylococcus aureus/efectos de los fármacosRESUMEN
The Eysenck Personality Questionnaire's (EPQ) N value (neuroticism) was used to evaluate information from 158 patients before ceramic crown treatment. Patient satisfaction was also evaluated using a satisfaction questionnaire 2 weeks post treatment. Patient expectations were not correlated with sex, age, or N value, and sex was not correlated with patient satisfaction other than in relation to crown shape. Total esthetic satisfaction and feature improvement were positively correlated with age, while satisfaction for five specific criteria was negatively correlated with N value and overall expectation. These observations underscore the importance of considering the physical and psychologic aspects of patient care when planning dental treatment.
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Trastornos de Ansiedad/psicología , Cerámica/química , Coronas/psicología , Materiales Dentales/química , Diseño de Prótesis Dental/psicología , Satisfacción del Paciente , Adulto , Factores de Edad , Estética Dental , Femenino , Humanos , Masculino , Persona de Mediana Edad , Neuroticismo , Coloración de Prótesis/psicología , Adulto JovenRESUMEN
BACKGROUND/AIM: Difficulties in the acceptance of dentures are multifactorial including psychosocial ones. It is questionable whether the patients' satisfaction depends only on the complete denture therapy characteristics. The aim of the study was to evaluated patients' expectation and satisfaction with complete dentures before and after the treatment concerning phonetics, chewing, comfort of use and aesthetics. METHODS: Forty complete edentulous patients rated their expectation before and satisfaction after the treatment based on a questionnaire scores. Patient-related variables regarding age, gender and previous experience (whether worn complete denture or not) were also recorded. RESULTS: Patients' rating for expectations were higher than the satisfaction after treatment regarding phonetics, chewing, comfort of use and aesthetics. A negative significant correlation was shown between the items before and after the treatment rating for phonetics, chewing, comfort of use and aesthetics. No statistical correlation was found between all the evaluated aspects' (i.e., phonetics, chewing, comfort of use and aesthetics) of expectation and satisfaction, and age, gender, and previous experience except a weak negative correlation noticed between age and comfort of denture use. CONCLUSION: Patients' expectations ratings significantly exceeded their satisfactions. Expectations and satisfaction ratings were irrespective of gender and patients previous experience.
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Dentadura Completa , Satisfacción del Paciente , Adulto , Anciano , Estética Dental , Femenino , Humanos , Masculino , Masticación , Persona de Mediana Edad , FonéticaRESUMEN
OBJECTIVE: To compare the shear bond strength(SBS) of cast Co-Cr alloys and selective laser melting(SLM) Co-Cr alloys with those of dental porcelain. METHODS: A dental porcelain (Vita) was applied on cast and SLM Co-Cr alloy specimens (n = 10). SBS test was conducted, and fracture mode analysis was determined. Student's t-test by SPSS 13.0 software was employed to analyze the data. RESULTS: The SLM Co-Cr alloy specimens had lower SBS values than the cast Co-Cr alloy specimens (P > 0.05). The metal-porcelain bond strength value of the cast group was (33.11 +/- 4.98) MPa, and that of the SLM group was (30.94 +/- 5.98) MPa. The specimens in both test groups exhibited mixed failure. CONCLUSION: The metal-porcelain system processed by SLM exhibit a bond strength that is similar to that of the cast group. This system also display a high precision.
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Porcelana Dental , Aleaciones de Cerámica y Metal , Aleaciones , Aleaciones de Cromo , Aleaciones Dentales , Rayos Láser , Ensayo de Materiales , MetalesRESUMEN
The purpose of this study was to evaluate the impact of psychological intervention on edentulous patients' satisfaction with complete clinically satisfactory complete dentures. The survey was conducted in China Medical University involving 84 individuals receiving complete dentures at this institution from August 2013 to March 2014. Participants were randomly allocated to intervention and control groups consisting of 42 subjects, respectively. In the intervention group, individuals received oral health education and psychological intervention before and after the whole process. In the control group, 42 cases received doctor's regular advice after treatment. Satisfaction regarding aesthetic, speech, mastication, retention and comfort was rated in the first and third month after prosthesis treatment. Patients overall aesthetic, speech, mastication and retention were significantly improved in both intervention and control groups in 3 months later after prosthesis treatment when compared with the first month (P < 0·05). No significant difference existed for comfort in control group. Patient scores on aesthetic, speech, mastication and retention at both first and third month evaluations (P < 0·05) differed between the control and intervention groups. However, there was no difference for their scores related to comfort (P > 0·05) in first and third month. The study concludes that psychological intervention plays a significant role in improving patient satisfaction with complete dentures.
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Dentadura Completa/psicología , Boca Edéntula/rehabilitación , Satisfacción del Paciente , Satisfacción Personal , Adulto , Anciano , Retención de Dentadura/psicología , Estética Dental/psicología , Femenino , Humanos , Masculino , Masticación/fisiología , Persona de Mediana Edad , Boca Edéntula/psicología , Calidad de Vida , Habla/fisiología , Encuestas y Cuestionarios , Resultado del TratamientoRESUMEN
OBJECTIVE: To evaluate the metal-ceramic bond strength of a selective laser melting Co-Cr alloy. METHODS: Twelve Co-Cr metal bars were prepared according to the ISO 9693 standard with Vita porcelain fused onto the centre of each bar. Then the sample bars were divided into two groups of six each. The control group was made by traditional cast process (cast group), and the experimental group was processed by selective laser melting (SLM) technology (SLM group). Metal-ceramic bonding strength and fracture mode were assessed using three-point bending test. Fracture mode analysis was determined by scanning electronic microscope/energy dispersive spectroscopy. Student's t-test was used to analyze the data in SPSS 13.0. RESULTS: The metal-ceramic bond strength value of the cast group was (33.45 ± 2.34) MPa, and that of the SLM group was (31.62 ± 2.34) MPa (t = 0.79, P > 0.05). A mixed fracture mode on the debonding interface of all specimens was observed, while little porcelain was reserved. CONCLUSIONS: The metal-ceramic system processed by SLM exhibited a bonding strength that satisfies the requirement of clinical application.
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Aleaciones de Cromo , Aleaciones de Cerámica y Metal , Fuerza Compresiva , Técnica de Colado Dental , Ensayo de MaterialesRESUMEN
OBJECTIVE: To compare curative effect between complete denture with lingualized occlusion and anatomic occlusion on temporomandibular joint disorders (TMD) of aged edentulous patients with severe residual alveolar ridge resorption. METHODS: Seventy aged edentulous patients with severe residual alveolar ridge resorption were recruited in this study and randomly assigned into two groups, thirty-five each. In Group A, patients received complete denture with lingualized occlusion, and in Group B, patients received complete denture with anatomic occlusion. The condition of TMD was examined and recorded by the same TMD specialist at baseline, 3 months and 6 months following denture delivery. The recovery effect of TMD was evaluated according to Fricton Index. Related data were analyzed statistically with t-test and rank sum test. RESULTS: Three months following denture delivery, the craniomandibular index (CMI) decrease value was (0.064 ± 0.022) in group A, and was significantly higher than that in group B (0.043 ± 0.018) (P < 0.01). Six months following denture delivery, the CMI decrease value was (0.084 ± 0.020) in group A, and was significantly higher than that in group B (0.070 ± 0.021) (P = 0.011<0.05). CONCLUSIONS: Complete denture with lingualized occlusion may be more conducive to the remission of TMD for aged edentulous patients with severe residual alveolar ridge resorption.
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Oclusión Dental , Dentadura Completa , Arcada Edéntula/complicaciones , Trastornos de la Articulación Temporomandibular , Anciano , Anciano de 80 o más Años , Oclusión Dental Céntrica , Femenino , Humanos , Masculino , Trastornos de la Articulación Temporomandibular/etiología , Trastornos de la Articulación Temporomandibular/terapiaRESUMEN
OBJECTIVE: To study the color reappearance effect of metal-ceram restoration and foundry-ceram restoration using Crystaleye spectrophotometer. METHODS: 58 metal-ceram restorations and 58 foundry-ceram restorations according to the result of the Crystaleye spectrophotometer were made respectively. The deltaE between restorations and natural teeth as referenced were analyzed. And satisfaction of dentists and patients were evaluated. RESULTS: The deltaE between metal-ceram restorations and natural teeth was 7.13 +/- 0.74. The deltaE between foundry-ceram restorations and teeth was 1.47 +/- 0.84. There were statistical differences between the deltaE (P < 0.01). The satisnatural faction of dentists and patients to the foundry-ceram restorations were higher than to the metal-ceram restorations (P < 0.05). CONCLUSION: Crystaleye spectrophotometer can provide accurate reference for foundry-ceram restoration, but for metal-ceram restoration it is not accurate.
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Color , Espectrofotometría , Humanos , Metales , DienteRESUMEN
OBJECTIVE: To investigate effects of the leaching liquids of 5 different kinds of dental alloys on L929 cells at cell level and molecular level. METHODS: The fibroblast L929 cells of mouse were cultivated in vitro in leaching liquids of 5 different kinds of dental alloys, Au alloy (n = 8), Ag-Pt alloy (n = 8), Co-Cr alloy (n = 8), Ni-Cr alloy (n = 8), and Cu alloy (n = 8). The RPMI 1640 cell medium containing 10% fetal beef serum was used as control. The cytotoxicities of the 5 dental alloys were evaluated by means of methyl thiazolyl tetrazolium (MTT), and the effects of these alloys on the expression of caspase-3, caspase-8, and caspase-9 mRNA of L929 cells were examined using reverse transcription polymerase chain reaction (RT-PCR) method. RESULTS: After 48 hours culture the cytotoxicity of Cu alloy group was in Grade 4 and those of the other groups were all in Grade 0. The mRNA levels of caspase-8 had no change in all groups (P > 0.05). The mRNA levels of caspase-3 were as follows: Cu alloy (0.474 +/- 0.001), the negative control (0.527 +/- 0.003), Au alloy (0.528 +/- 0.013), Co-Cr alloy (0.615 +/- 0.007), Ag-Pd alloy (0.673 +/- 0.009), and Ni-Cr alloy (0.803 +/- 0.037). The mRNA levels of caspase-9 were as follows: Cu alloy (0.532 +/- 0.041), Au alloy (0.574 +/- 0.013), the negative control (0.578 +/- 0.010), Co-Cr alloy (0.617 +/- 0.009), Ag-Pd alloy (0.703 +/- 0.018), and Ni-Cr alloy (0.811 +/- 0.037). There were significant differences between the groups except the negative control group and Au alloy group. CONCLUSIONS: The Cu alloy shows the highest cytotoxicity, and the leaching liquids of 5 different kinds of dental alloys may induce cell apoptosis through mitochondrion pathway.