[Acclimatization and Community Structure Analysis of the Microbial Consortium in Nitrate-Dependent Anaerobic Methane Oxidation].

Methane oxidation coupled with denitrification is an effective way to reduce the discharge of nitrate and methane. The anaerobic sludge from a laboratory wastewater treatment facility, anaerobic sludge from a wastewater treatment plant, and soil from a landfill were selected as inoculum to enrich the consortium for anaerobic methane oxidation in cooperation with nitrate reduction. The investigation of methane oxidation was carried out in these systems. The results showed that the maximum methane consumption rate of 0.05 mg·d-1 was obtained when the anaerobic sludge from a wastewater treatment plant served as inoculum. The population of bacteria and archaea were assayed by the clone library method. The Methanosarcinales and Methanomicrobiales were present in methane oxidation as methane oxidizing archea. The Pseudomonas, Clostridia, and Thermomonas were identified as nitrate reducing bacteria in the process of nitrate reduction. Both the methane conversion rate and microbial population varied with the amount of nitrate. The nitrate reduction bacteria were Pseudomonas and Clostridia when the nitrate concentration was 200 mg·L-1. The Pseudomonas and Thermomonas emerged when the nitrate concentration increased to 500 mg·L-1, and the rate of methane conversion was increased by 34.7%. The results provided science evidence for the co-treatment of methane and nitrate.

[F10 expressions in cervical cancer tissues].

OBJECTIVE: To detect the expression of F10 at both mRNA and protein levels in cervical cancer tissues and explore its role in the occurrence and progression of cervical cancer. METHODS: F10 expressions at mRNA and protein levels were detected in 30 pairs of cervical cancer tissues and adjacent tissues using RT-PCR and immunohistochemistry. RESULTS: The mRNA and protein expressions of F10 were significantly higher in cervical cancer tissues than in the adjacent normal tissues (P<0.05). F10 expression was significantly higher in poorly differentiated cervical cancer than in well differentiated cancer tissues, and was also lower in patients with preoperative chemotherapy than in those without chemotherapy. CONCLUSION: F10 expression level is inversely correlated with the differentiation of cervical cancer and possible plays a role in the tumorigenesis and progression of cervical cancer.

[Surface characteristics of alkali modified activated carbon and the adsorption capacity of methane].

Coconut shell based activated carbon was modified by alkali with different concentrations. The surface structures of tested carbons were observed and analyzed by SEM and BET methods. Boehm's titration and SEM/EDS methods were applied to assay the functional groups and elements on the carbon surface. The adsorption of methane on tested carbons was investigated and adsorption behavior was described by the adsorption isotherms. Results showed that surface area and pore volume of modified carbon increased and surface oxygen groups decreased as the concentration of the alkali used increased, with no obvious change in pore size. When concentration of alkali was higher than 3.3 mol x L(-1), the specific surface area and pore volume of modified carbon was larger than that of original carbon. Methane adsorption capacity of alkali modified carbon increased 24%. Enlargement of surface area and pore volume, reduction of surface oxygen groups will benefit to enhance the methane adsorption ability on activated carbon. Adsorption behavior of methane followed the Langmuir isotherm and the adsorption coefficient was 163.7 m3 x mg(-1).