Diet supplementation with hemp (Cannabis sativa L.) inflorescences: effects on quanti-qualitative milk yield and fatty acid profile on grazing dairy goats.

Hemp (Cannabis sativa L.) is an annual plant belonging to the family of Cannabaceae with several varieties characterized by different fatty acid profile, content in flavonoids, polyphenols, and cannabinoid compounds. Hemp is mostly used in livestock nutrition as oil or as protein cake, but not as inflorescences. The aim of this study was to evaluate the effect of dietary hemp inflorescences on milk yield and composition in grazing dairy goats. Twenty Camosciata delle Alpi goats at their 3rd parity and with a mean body weight of 45.2 ± 2.0 kg, immediately after kidding, were equally allocated into two groups (G: Grazing and GH: grazing and hemp). For three months, all goats were fed on a permanent pasture and received 700/head/day of concentrate; diet of group GH was supplemented with 20 g/head/day of hemp inflorescences. Goats' body weight did not change during the trial. Individual milk yield was daily recorded and samples collected every 20 days for chemical composition and fatty acid profile analysis. No significant differences were found for milk yield and chemical composition. Caproic (C6:0) (1.80 vs. 1.74%; p < 0.01) and lauric acids (C12:0) were significantly higher in milk of group GH (4.83 vs. 4.32%; p < 0.01) as well as linoleic (C18:2) (2.04 vs. 1.93%; p < 0.05), adrenic acid (C22:4) (0.046 vs. 0.031%, p < 0.05), omega-6/omega-3 ratio (3.17 vs. 2.93, p < 0.05) and total conjugated linoleic acids (CLAs) (0.435 vs. 0.417%; p < 0.01). The results of this study suggest that the supplementation of grazing goats' diet with hemp inflorescences may enhance the milk nutritional characteristics by increasing its content of CLAs and other beneficial fatty acids.

The Effects of Hemp Hay (Canapa sativa L.) in the Diets of Grazing Goats on Milk Production and Fatty Acid Profile.

Hemp (Cannabis sativa L.) is a cosmopolitan annual herbaceous plant used in the past as a source of textile fiber. Currently, hemp is receiving great interest as animal feed due to its chemical and nutritional properties. The aim of this study was to explore the effects of supplementing goats' diets with hemp hay on the milk yield, chemical composition, and fatty acid profile. Twenty multiparous goats, immediately after kidding, were divided into two homogenous groups (C: control vs. H: hemp); the goats had free access to the pasture, and both groups received a supplement of 500 g/head/day of a barley and corn meal mixture (50/50). In addition, group H was given 250 g/head/day of hemp hay while group C received the same amount of alfalfa hay. The milk yield was measured daily, and milk samples were collected monthly 4 times to evaluate the milk composition and fatty acid profile. The milk yield was significantly (p < 0.05) higher in the experimental group, while no differences were found in the milk chemical composition. Concerning the fatty acid profile, the milk from group H was characterized by significantly lower concentrations of C11:0, C12:0, C13:0, C14:0, C15:0, and C17:0 and higher C16:0 and C18:0. Among the polyunsaturated FA, C18:2 n6 and C20:4 were significantly (p < 0.001) lower, and C20:5 n3 was significantly (p < 0.05) higher in the milk from group H than that from group C. The n6/n3, LA/ALA and AA/EPA ratios were significantly (p < 0.001) lower in the milk from group H, while the CLAs were unaffected by the treatment.

Growth Performance of Buffalo Calves in Response to Different Diets with and without Saccharomyces cerevisiae Supplementation.

The aim of the present trial was to evaluate the growth performance of buffalo calves fed on diets characterized by different forage/concentrate ratios, with or without Saccharomyces cerevisiae supplementation (CBS 493.94, Yea-Sacc®). Twenty-four male buffalo calves (mean age of 145.1 ± 16.1 days; mean weight of 108.0 ± 18.7 kg) were assigned randomly to 4 groups, homogeneous in age, that were fed four different diets: diet 1, F:C ratio 50:50; diet 2, F:C ratio 30:70; diet 3, F:C ratio 50:50 + Yea-Sacc®; and diet 4, F:C ratio 30:70 + Yea-Sacc®. Buffalo calves were individually weighted before the start of the experiment and the data were used as a covariate, being taken monthly until the end of the trial. Dry matter intake (DMI), daily weight gain (DWG) and feed conversion ratio (FCR) were calculated. The differences in diets composition significantly (p < 0.01) affected all these parameters. In particular, the animals fed diet 1 and diet 3 showed higher values of DWG (0.91 and 0.88 g/d vs. 0.68 and 0.66 for group 2 and 4) and DMI (5.8 and 5.3 kg/d, respectively) compared to the other groups (4.3 and 4.4 kg/d for group 2 and 4), as well as a higher final body weight (370.5 and 334.1 kg for group 1 and 3 vs. 272.8 and 273.1 kg of group 2 and 4, respectively). Indeed, the supplementation with Yea-Sacc® at the dosage of 1 × 10E8 did not affect buffaloes' growth performance.

A Comparative Study of Dogs and Goats with Limited Human Socialization in the Impossible Task Paradigm.

The study aimed to explore how limited human socialization affects the socio-cognitive abilities and interactions with unfamiliar individuals of a selected group of domesticated dogs and goats. These animals were raised and kept under conditions characterized by limited human socialization, and their behavior was assessed using the "impossible task" paradigm. The study found that dogs, with a history of cooperative interactions and human companionship, exhibited more frequent social engagement with human experimenters in the experimental setting than goats, traditionally domesticated for utilitarian purposes. However, differences in interaction duration and latency were not significant, highlighting the complexity of these interactions. The results suggest that domestication history and behavioral ecology play significant roles in shaping animals' willingness to engage with humans. However, this study acknowledges limitations, such as the specific population studied, and calls for further research with larger and more diverse samples to generalize these findings. Understanding the interplay between domestication history, behavioral ecology, and human socialization could provide insights into the complex factors influencing animal-human interactions and cognitive behaviors, with implications for animal welfare and human-animal relationships.

Forage:Concentrate Ratio Effects on In Vivo Digestibility and In Vitro Degradability of Horse's Diet.

Determination of digestibility represents the first step for the evaluation of the net energy content of feed for livestock animals. The aim of this study was to evaluate the in vivo digestibility and in vitro degradability of five diets characterized by different forage/concentrate ratios (F:C) in horses. The in vitro degradability was determined by the Gas Production Technique (GPT), using as an inoculum source the feces of the same subjects used for the in vivo test. Five diets consisting of poliphyte hay, straw and grains of barley and oats with a different F:C ratio [90/10 (Diet 1); 78/22 (Diet 2); 68/32 (Diet 3); 60/40 (Diet 4); 50/50 (Diet 5) were formulated and administered in succession, starting with Diet 1. In the in vivo results, no significant differences emerged, despite the different F:C content. In in vitro fermentation, four diets out of the five (2, 3, 4, 5) presented a similar trend of the curve of gas production, showing good activity of the fecal micro population during the first hours of incubation. An important correlation between gas and Volatile Fatty Acid (VFA) were found, suggesting that the processes linked to the micro population deriving from the horse's caecum follow metabolic pathways whose products can be modeled in the same way as for the rumen. The GPT could represent the correct method for studying the nutritional characteristics of feed for horses, using feces as the source of inoculum, even if further investigations must be performed to improve the technique.

Colostrum Composition, Characteristics and Management for Buffalo Calves: A Review.

In this review, the composition, characteristics, and management of dairy buffalo calves were examined and compared with bovines. The neonatal period is critical for buffalo calves and is characterized by a high mortality rate (more than 40%). The early intake of high-quality colostrum (IgG > 50 mg/mL) is the one way to improve the immune system of calves (serum IgG > 10 mg/mL after 12 h), thus increasing their chances of survival. Mainly in intensive farms, the availability of high-quality colostrum is necessary; thus, good quality colostrum is often stored to provide newborn calves which cannot be fed by their mothers. Also, the manipulation of the immunological status of animals through vaccination has been depicted since the quality of colostrum tended to be influenced by vaccination against pathogens. Buffalo breeding is constantly expanding in Italy, mainly thanks to the Mozzarella cheese production that represents the excellence of the "Made in Italy" and is exported worldwide. Indeed, high calf mortality rates directly affect the profitability of the business. For these reasons, the aim of this review was to examine specific research on buffalo colostrum that, compared with other species, are scarce. Improving the knowledge of buffalo colostrum, in terms of characteristics and management, is critical to guarantee buffalo newborns' health in order to reduce their mortality rate. Importantly, considering the knowledge on cattle valid also for buffalo is a widespread, and often erroneous, habit in several fields, including colostrum feeding. Therefore, the two species were compared in this review.

Nutritional Characterization of Hay Produced in Campania Region: Analysis by the near Infrared Spectroscopy (NIRS) Technology.

Since the dietary characteristics of hays can be very variable, it is of great importance for nutritionists to know their chemical composition in order to formulate adequate rations for the animals. Laboratory analyses are time-consuming and expensive while the Near Infrared Spectroscopy offers several advantages, including obtaining information on feeds nutritional characteristics very quickly and in situ at the farm, thanks to portable NIRS. In this trial, over 400 hay samples collected in the Campania region (Italy) were analyzed with portable NIRS device. The final aim was to analyze the differences in hay quality in different production areas, highlighting the possible factors involved and suggesting possible corrective measures. All the analyzed hays (polyphite, Gramineae and alfalfa) were significantly (p < 0.05) influenced by the area of cultivation/preservation. In particular, the polyphite and Gramineae hays produced in most of the areas of Campania region showed poor nutritional value due to the low protein content and high structural carbohydrate that significantly reduced its digestibility. The use of high-quality forages in the ration represents a pivotal factor to allow the production of high-quality products of animal origin. The use of NIRS seems to be a valuable strategy to select potential treatments that can increase feed digestibility and to avoid long chemical analysis.

In vitro fermentation characteristics of diets with different forage/concentrate ratios: comparison of rumen and faecal inocula.

BACKGROUND: The aim of this trial was to evaluate the replacement of rumen fluid with faeces as inoculum in studying the in vitro fermentation characteristics of diets for ruminants using the in vitro gas production technique. Six iso-protein diets with different forage/concentrate ratios were incubated with rumen fluid (RI) or faeces (FI) collected from sheep. RESULTS: Most of the fermentation parameters were influenced by diet and inoculum (P < 0.01). With both inocula, organic matter degradability (dOM), cumulative gas production (OMCV) and maximum fermentation rate (R(max) ) increased as the amount of concentrate in the diet increased. R(max) was lower with FI vs RI (P < 0.01); dOM was higher with FI vs RI and the diet × inoculum interaction was significant. As expected, with both inocula, R(max) increased as the neutral detergent fibre content of the diet decreased. Significant correlations were obtained using both inocula between OMCV/dOM and gas/volatile fatty acid (VFA), while the correlation VFA/dOM was significant only with FI. The microbial biomass yield calculated by stoichiometric analysis for all diets was higher with FI vs RI. With FI the organic matter used for microbial growth showed an overall decreasing trend as the amount of concentrate in the diet increased. CONCLUSION: The results indicate that both faeces and rumen fluid from sheep have the potential to be used as inoculum for the in vitro gas production technique.

Influence of rumen protein degradability on productive and reproductive performance in buffalo cows.

The present study aimed to ascertain the influence of crude protein (CP) digestibility in the rumen on the quantity and quality of milk production and reproductive performance, blood (BU) and milk (MU) urea, haematological profile and vaginal mucus urea, ammonia and potassium of buffalo cows. Lactating buffaloes (n = 84), 60 days in milk, were randomly subdivided into Group C (control, n = 42) and Group T (fed a diet supplemented with Aspergillus oryzae, n = 42). In three fistulated buffalo, the diet supplemented with Aspergillus oryzae showed a decrease (P < 0.01) in protein digestibility in the rumen (79.3 vs. 45.9%). No differences were registered in productive performance. Nine buffaloes not in oestrus during the dietary treatment (Groups T1 and C1), 30 days in milk, were used to study the haematological profile and to determine milk urea and ammonia in the vaginal mucus. The animals in Group T1 had higher ammonia values in the blood (P < 0.01) but not in the vaginal mucus than Group C1. A relationship was found between MU and BU. MU was influenced by CP intake and dry matter intake. No differences between the treatments were observed in reproductive performance and the conception rate and calving interval were 37.9% and 41.4% (90 trial-day) and 449 and 419 days respectively in Groups T and C. Reproductive performance was not influenced by high levels of BU nor by blood ammonia levels, although the latter were higher in the group fed the diet supplemented with Aspergillus oryzae.