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Innovations in medical education, including the integration of narrative-based tales, are transforming the way complex biochemical concepts are taught and understood. In this "Idea to Explore", the essence of integrating tales that personify molecules and depict biochemical processes as engaging stories to enhance student engagement, promote active learning, and improve knowledge retention is discussed. It also explores the effectiveness of scientific discovery games and traditional scientific stories in deepening students' interest in biochemistry. Highlighting the potential of narrative methods to make biochemistry more accessible and engaging, educators are encouraged to adopt creative teaching tools that promote critical thinking, problem-solving, and communication skills, thereby inspiring active participation, and lifelong learning in biochemistry.
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Educación Médica , Estudiantes de Medicina , Humanos , Aprendizaje Basado en Problemas , Pensamiento , Bioquímica/educaciónRESUMEN
AIM: To describe pregnancy outcome of kidney transplant patients till 1 year postpartum. METHODS: This retrospective, monocentric study included 15 kidney transplant patients who presented 18 pregnancies, between January 2000 and January 2020. For each of them, we searched for possible obstetrical, fetal and renal complications and we evaluated renal function before, during and after pregnancy. RESULTS: The live birth rate was 84% (16/19) with an average gestational age at delivery of 37 weeks of gestation. The rate of prematurity was 50% (8/16), gestational diabetes was 16.6% (3/18) and preeclampsia was 27.7% (5/18). Cesarean section was performed in 61.1% (11/18) of cases including, 81.8% (9/11) unplanned surgery. The average birth weight was 2635 grams and 37.5% (6/16) of the newborn were small for gestational age. All patients had stable renal function before conception of pregnancy. We noticed two acute graft rejection during pregnancy with only one resulting in graft loss. Four patients had a reduced graft function in 12months of the postpartum. CONCLUSION: Risk of maternal, fetal and renal complications remained high in kidney transplant recipients. Pregnancy should be carefully planned in transplanted women associated with adequate follow-up according to clinical guidelines (normal renal function and blood pressure without proteinuria before pregnancy, no recent graft rejection, period of one year after transplant respected and no teratogenic treatment in the month before pregnancy).
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Cesárea , Trasplante de Riñón , Complicaciones del Embarazo , Resultado del Embarazo , Humanos , Femenino , Embarazo , Estudios Retrospectivos , Adulto , Complicaciones del Embarazo/epidemiología , Cesárea/estadística & datos numéricos , Recién Nacido , Maternidades , Preeclampsia/epidemiología , Rechazo de Injerto/epidemiología , Edad Gestacional , Diabetes Gestacional/epidemiología , Peso al Nacer , Nacimiento VivoRESUMEN
BACKGROUND: The recent development of the Global TALES Protocol provides a unique opportunity to conduct systematic cross-linguistic and cross-cultural comparisons of children's personal narratives. This protocol contains 6 scripted prompts to elicit personal narratives in school-age children about times when they experienced feeling happy/excited, worried, annoyed, proud, being in a problem situation, something important. OBJECTIVES: The objectives of this study were to examine the topics of the children's narratives when they responded to the 6 prompts and draw comparisons with the topics of narratives spoken by children from 10 other countries speaking 8 other languages as described in the original feasibility paper. METHODS: We translated the Global TALES Protocol into Hindi and collected personal narratives of thirty Hindi-speaking children (aged 6-9 years), residing in Varanasi, Uttar Pradesh, India. All personal narrative samples were elicited in person and audio recorded for manual coding of the topics. RESULTS: Although we observed many similarities in the topics of children's personal narratives between this dataset and the dataset reported on in the initial feasibility study, we also documented some novel topics, such as "welcoming guests" in response to the "excited" prompt; "financial problems" in response to the "worried" prompt; "helping someone by actions or by advising someone morally" in response to the "problem" prompt; and "mishap/personal loss" and "exams" in response to the "important" prompt. CONCLUSION: Some of these novel topics likely reflected the Indian culture. Because our study involved a group of children who are linguistically and culturally different from previous studies using the Global TALES protocol and, at ages 6-9 years, slightly younger than the 10-year-olds in prior studies, this study adds to the evidence that the Global TALES protocol can be used to elicit personal narratives of children from diverse languages and cultures, as young as age 6.
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Lenguaje , Narración , Humanos , Niño , Lenguaje Infantil , Lingüística , IndiaRESUMEN
INTRODUCTION: Tele-assessment (TA) has the potential to enhance access to speech therapy. This preliminary study aimed to investigate the impact of assessment mode (face-to-face [FTF] vs. TA) on the microstructure level and chosen topics of personal narratives produced by Arabic-speaking and Hebrew-speaking school-age children living in Israel. We also investigated whether performance variations, if evident, could be attributed to the children's language/culture. METHODS: Eighty-nine 10-year-old children, 38 Arabic-speaking and 51 Hebrew-speaking, living in Israel, participated in this study. Forty participants were assigned to a TA group (via Zoom) and 49 to a FTF group. All participants were assessed using the Global TALES protocol, generating six personal narratives each. The narratives were analyzed regarding the following microstructural measures: total number of words, total number of utterances (TNU), number of different words, and mean length of utterance in words (MLU-W). Additionally, each narrative was categorized into a topic according to the Global TALES protocol. RESULTS: The analysis revealed no significant main effect of assessment mode on any of the microstructure measures. However, a significant interaction effect between language/culture and assessment mode was found for TNU and MLU-W, with a significant main effect for TNU exclusively in the Arabic narratives, with the Arabic-speaking children producing more utterances through FTF compared to TA. Across language/culture groups, there was a significant effect of assessment mode on the chosen topic. Additionally, there were significantly higher scores in the Hebrew compared to the Arabic narratives in all microstructure measures, and language/culture also influenced the chosen topics. CONCLUSIONS: The results support the feasibility of TA mode for assessing personal narratives in school-aged children, using the Global TALES protocol. However, the results also suggest that TA results may be affected by the language/culture of the narrator. Finally, the findings highlight the potential influence of TA on the chosen topics of personal narratives, perhaps due to a decrease in the quality of communication in TA.
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Desarrollo del Lenguaje , Lenguaje , Niño , Humanos , Comunicación , Lenguaje Infantil , NarraciónRESUMEN
INTRODUCTION: Personal narratives are monological stories based on a personal experience that help children explain and understand their emotional states, as well as process positive and negative experiences. The aim of this study was to identify age- and emotion-related traits of lexical and grammatical abilities and coherence of personal stories produced by school-aged children between 7 and 13 years. METHODS: A total of 60 typically developing children, speakers of Croatian, were stratified into three groups according to age. Using the Global TALES protocol, each child was asked to produce six personal stories prompted by different emotional states. The personal narratives were analysed using measures of lexical diversity (lemma-token ratio and number of different words), productivity (total number of words), and syntactic complexity (mean length of utterances and clausal density). Based on the Narrative Coherence Coding Scheme, three coherence dimensions (context, chronology, and theme) were rated. RESULTS: Age group was shown to explain 18% of the variance in the ability to produce personal narratives. Personal narratives elicited through positive prompts were overall more lexically diverse but were significantly less elaborated chronologically and thematically than negative and neutral narratives. CONCLUSION: This study showed that coherence of the produced stories was connected with the child's lexicon and that both variables - lexicon and coherence - were influenced by emotional valence of the story. In contrast, grammatical aspects of the narrative were influenced only by age. Finally, it is possible to state that the Global TALES protocol is sensitive enough to capture specificities of creating personal stories, both developmental ones and those created under the influence of the emotional valence of the prompts.
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Emociones , Narración , Humanos , Niño , Adolescente , Estudios TransversalesRESUMEN
INTRODUCTION: This study examines whether there are differences in expressive vocabulary between participants with and without dyslexia in personal narratives in response to the Global TALES protocol. METHODS: 22 monolingual Dutch-speaking participants aged 11-16 with dyslexia and 22 age and gender-matched peers without dyslexia were assessed on measures of decoding, reading comprehension, and spelling of words, pseudowords, verbs, and sentences. The participants also produced personal narratives in response to the six prompts contained in the Global TALES protocol. We analyzed the personal narratives for expressive vocabulary and counted the total number of different words (TNDW). RESULTS: The study revealed a significant relationship between TNDW and reading comprehension (r = 0.45, p = 0.002, BF10 = 17.70), spelling words (r = 0.42, p = 0.005, BF10 = 8.93), and spelling and writing conventions in sentences (r = 0.37, p = 0.016, BF10 = 3.11). The Global TALES protocol was successful in eliciting personal narratives in the Dutch-speaking participants with and without dyslexia. Participants with dyslexia used fewer different words (M = 192.27, SD = 64.37; 95% CI: [151.84-232.71]) compared to peers without dyslexia (M = 265.50, SD = 116.28; 95% CI: [225.06-305.93]; F(1, 42) = 6.68; p = 0.013; η2 = 0.14). When we compared the probability of models, Bayesian factors revealed moderate evidence for group differences in TNDW (BF = 3.94). CONCLUSION: Our findings indicate that older school-age participants with dyslexia may lag behind their peers in expressive vocabulary in a personal narrative discourse task that is relevant to everyday functioning. The results of this study highlight the relationship between expressive vocabulary and reading comprehension and the importance of the assessment of spoken language skills in children with dyslexia. Reading problems might lead to less advanced spoken language, which in turn may negatively affect the expressive vocabulary growth in individuals with dyslexia.
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Dislexia , Vocabulario , Niño , Humanos , Teorema de Bayes , Lenguaje , Lectura , EstudiantesRESUMEN
INTRODUCTION: Studies on personal narratives are rare in Turkey and there is no standard protocol for eliciting them. The aim of this small-scale study was to translate the Global TALES Protocol into Turkish, with cultural adaptations, and to present the results regarding its usability for two different age-groups of 7- and 10-year-old school children. We investigated narrative skills in terms of verbal productivity (number of utterances, total number of words), syntactic complexity (mean length of utterance), and semantic diversity (number of different words). In addition, group comparisons were made in terms of the participants' gender and age. METHODS: A total of 20 children, 10 from each age-group (7;0-7;11 and 10;0-10;11) participated in the study. All children were monolingual Turkish-speaking children with typical development. Participants were recruited through personal and/or social networks. All personal narratives were gathered via online connections (Zoom). RESULTS: Descriptive statistics were used to describe the children's performance, and the analysis of group differences was made separately according to age and gender. All children produced narratives in response to the six protocol prompts. In addition, the number of children who did not require the scripted follow-up prompts was higher than those needing a scripted follow-up prompt to produce a response. No statistically significant group differences were found in terms of gender and age on any of the measurements. CONCLUSION: The results from this small-scale investigation showed that the translated version of the Global TALES Protocol was effective in eliciting personal narratives from Turkish-speaking children. We concluded that there is no need to change the directions or give additional guidance or prompts to the children. Future studies with larger samples are needed to confirm these findings.
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Narración , Semántica , Humanos , Niño , Proyectos Piloto , TurquíaRESUMEN
INTRODUCTION: Cypriot Greek is the variety of Greek language used for oral communication by the Greek Cypriot people, while Standard Modern Greek is the official language. Cypriot Greek differs from Standard Modern Greek in various aspects, including lexicon, phonetics, phonology, morphosyntax, and pragmatics. This study examines whether there are differences between children who are native speakers of the Cypriot Greek dialect and those who are native speakers of Standard Modern Greek in narrative measurements obtained in a personal narrative context. METHODS: Thirty-nine ten-year-old children (19 Cypriot Greek speakers and 20 Standard Modern Greek speakers) participated in the study. The Global TALES protocol was used to elicit personal narratives across 6 emotion-based prompts. Measures of microstructure (mean length of utterance, number of different words) and macrostructure (plot elements) are examined. RESULTS: Analysis showed no differences in macrostructure between Cypriots Greek speakers and Standard Modern Greek speakers in response to the prompts. In contrast, group differences were found in syntactic complexity, with the Standard Modern Greek speakers producing longer sentences. CONCLUSION: This is the first study to compare the characteristics of personal narratives spoken by 10-year-old children who are speakers of Cypriot Greek and Standard Modern Greek. The results from this initial comparison highlight that children who are speakers of two varieties of the same language share similar narrative elements. However, they may differ in their linguistic profiles, such as in syntax. Understanding these differences is important for researchers, educators, and speech therapists. A detailed understanding of developmental milestones and a clear understanding of each variety's characteristics are crucial from a research, pedagogical, and speech therapy perspective.
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Lenguaje , Fonética , Niño , Humanos , Grecia , Lenguaje Infantil , NarraciónRESUMEN
INTRODUCTION: The feasibility of using a standard protocol, labelled as the Global TALES, to elicit personal stories was tested across 10 different countries between 2019 and 2021. Personal narratives have not been investigated in Iceland in a similar way. The aim was to explore personal narratives of monolingual children in Iceland by using the Global TALES (2018) protocol to investigate verbal productivity, semantic diversity, and narrative topics. METHODS: Twenty-seven children (ages 9; 8-10; 9) with typical language development produced personal stories in response to six emotion-based prompts contained in the protocol. The children were interviewed by using the Zoom platform because of COVID restrictions. Children's spoken language was analysed for total number of utterances (TNU), total number of words (TNW), number of different words (NDW), mean length of utterances in words (MLUw), and proportion of words with grammatical errors. The narrative topics were coded and documented. RESULTS: The spoken language measures showed that there was a large individual variability within the group on all metrics examined. The mean TNU was 62.1 (SD 20.2) utterances, the mean for TNW was 546 (SD 219) words, the mean for NDW was 206 (SD 62) words, and the MLUw was 8.6 (SD 1.5) words in an utterance. Despite the variability in spoken language performance, around 70-75% of the participants produced TNU, TNW, NDW, and MLUw within +/- 1 SD from the mean. The topics of the stories were most frequently related to personal achievement or relationships with the family or friends. CONCLUSION: The Global TALES (2018) protocol can be used to elicit personal stories in 10-year-old children in Iceland. The results related to verbal productivity and the topics of interest were in line with previous studies around the world.
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Lenguaje Infantil , Desarrollo del Lenguaje , Humanos , Niño , Islandia , Semántica , NarraciónRESUMEN
INTRODUCTION: It has been well established that the function of sharing personal narratives is to inform the listener about what the event meant to the narrator, for example, by using a range of evaluative devices. The use of these evaluative devices may reflect a person's understanding of the differences between one's own mind and others, by expressing their beliefs, emotions, thoughts, and desires. This paper investigates children's use of evaluative devices when producing personal narratives in response to the six emotion-based prompts contained in the Global TALES protocol (excited, worried, annoyed, proud, problem situation, something important). It addresses three research questions: (1) What types and proportions of evaluative devices do 10-year-old English-speaking children use in response to the six prompts contained in the Global TALES protocol and are there differences in performance between boys and girls? (2) What are the correlations between the different types of evaluative devices? (3) Does children's use of evaluative devices differ depending on the type of prompt used? METHODS: Eighty-two native English-speaking ten-year-old children from three English-speaking countries (Australia, New Zealand, and the USA) participated. None of the children had been identified with language and/or learning difficulties. Children's personal narratives were transcribed and analysed for the use of 12 evaluative devices: compulsion, internal emotional states, evaluative words, intensifiers, mental states, causal explanations, hypotheses, objective judgements, subjective judgements, intent, negatives, and repetition. RESULTS: Results showed that children use a high number of evaluative devices, with "intensifiers" and "evaluative words" used most frequently. There were few effects for sex, apart from girls using a wider range of evaluative devices than boys. We found moderate to large correlations between most devices, with factor analysis revealing three factors we labelled "causality," "hypothesis," and "judgement." Although there were significant overall effects for prompt type on the use of evaluative devices, there was no clear pattern when inspecting responses to individual prompts. CONCLUSION: The results from this study shed light on children's use of evaluative devices to convey the meaning of their personal narratives in response to six different prompts tapping into different emotions. Moving beyond appraising children's structural language skills when narrating their personal experiences may enhance the understanding of interpersonal and intrapersonal aspects of theory of mind, which may inform clinical practices, such as individualized goal setting and intervention choices.
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Emociones , Lenguaje , Masculino , Femenino , Humanos , Niño , Desarrollo del Lenguaje , Lenguaje Infantil , CogniciónRESUMEN
INTRODUCTION: This small-scale study explored the feasibility of the Global TALES protocol in eliciting personal narratives in typically developing monolingual Irish children, using the online Zoom platform. We investigated children's performance on measures of productivity (total number of utterances; total number of words) and syntactic complexity (MLU in words). We also documented the topics children talked about in response to the six emotion-based prompts contained in the Global TALES protocol. METHODS: Nineteen typically developing children (6 male, 13 female), aged between 10.0 and 10.11 years produced personal narratives in response to the Global TALES protocol. Given COVID-19 pandemic-related public health restrictions, the language samples were elicited using Zoom. All stories were transcribed and analysed using Systematic Analysis of Language Transcripts software. Qualitative content analysis was used to code the topics of the children's stories. RESULTS: Sixteen participants responded to all prompts. One participant only responded to three of the six prompts. The prompt that was least successful in eliciting a response was the "problem" prompt; 15.7% (n = 3) of the children did not provide a response to this prompt. On average, children produced 40 utterances, although individual variability was high. On average, MLU was 8.7, ranging from 6 to 11. Children's topics closely resembled those reported in the Global TALES feasibility study despite the fact, the current study took place during the COVID-19 pandemic. The most frequent topics were related to family (events, illnesses, relationships, siblings) and finding or fixing something. CONCLUSION: The Global TALES protocol was successful in eliciting personal narratives from 10-year-old Irish English-speaking children. Future larger scale studies are now needed to investigate if the results generalise to the wider Irish population with a view to create local benchmarks of personal narrative performance.
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COVID-19 , Pandemias , Humanos , Niño , Masculino , Femenino , Irlanda , Estudios de Factibilidad , Narración , Lenguaje InfantilRESUMEN
This paper, at the intersection of literary studies, computational literary analysis and psychology, analyzes Lovecraft's literary production throughout his career. Fifty-four writings from the period 1905-1935, which span almost all of his career as a fiction writer, have been studied by applying the computational techniques of sentiment analysis and following a methodology structured in three stages. In the first one, I analyzed each of the writings, in order to identify the relative percentage of emotion-inducing words according to Plutchik's Wheel of Emotions. The second phase of this process consisted of hierarchical cluster analysis to identify homogeneous groups of works. Finally, in the third step of the research, I conducted a statistical analysis. The results point to three different conclusions. First, the quantitative analysis confirms that there exists an intimate connection between the emotions of fear and sadness in Lovecraft's writings, which generates an intense emotion of despair. Second, the vast majority of his works can be grouped into two categories, each with a distinct pattern in terms of emotions. Finally, there seems to be a relationship between the identified categories and the evolution of Lovecraft's style; that is, there is a temporal evolution toward much darker works, which are dominated by negative emotions.
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INTRODUCTION: Xa23 as an executor mediates broad-spectrum resistance to Xanthomonas oryzae pv. oryzae (Xoo), which contains a matching avirulence gene avrXa23, in rice for bacterial leaf blight (BLB). avrXa23 encodes a transcription activator-like effector (TALE) protein which binds to the EBE (effector-binding element) of the Xa23 promoter. It is unclear whether the considerable pressure of Xa23 leads to an emerging Xoo strain that overcomes Xa23 resistance. OBJECTIVES: This study aimed to uncover new Xoo isolate(s) that overcome Xa23-mediated resistance and to investigate how the pathogen evades the resistance. METHODS: Totally 185 Xoo isolates were used to screen possibly compatible strain(s) with Xa23-containing rice CBB23 by pathogenicity test. Genome Sequencing, Southern blot, tal gene cloning, Western blot, qRT-PCR and electrophoretic mobility shift assays (EMSA) were conducted to determine the mechanism of one Xoo isolate being compatible with Xa23-containing rice. RESULTS: One isolate AH28 from Anhui province is compatible with CBB23. AH28 strain contains an ortholog of avrXa23, tal7b and has 17 tal genes. The 4th RVD (repeat-variable diresidue) in Tal7b are missed and the 5th and 8th RVDs changed from NG and NS to NS and S*, respectively. These alternations made Tal7b unable to bind to the EBE of Xa23 promoter to activate the expression of Xa23 in rice. The ectopic expression of tal7b in a tal-free mutant PH of PXO99A did not alter the virulence of the strain PH, whereas avrXa23 made AH28 from compatibility to incompatibility with Xa23 rice. CONCLUSION: Best to our knowledge, this is the first insight of a naturally-emerging Xoo isolate that overcomes the broad-spectrum resistance of Xa23 by the variable AvrXa23-like TALE Tal7b. The RVD alteration in AvrXa23 may be a common strategy for the pathogen evolution to avoid being "trapped" by the executor R gene.
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Oryza , Oryza/genética , Oryza/metabolismo , Efectores Tipo Activadores de la Transcripción/genética , Efectores Tipo Activadores de la Transcripción/metabolismo , Enfermedades de las Plantas/genética , Enfermedades de las Plantas/microbiología , Regulación de la Expresión Génica de las PlantasRESUMEN
Xanthomonas is a highly evolved group of phytopathogenic bacteria infecting nearly 400 host plants having vast genomic resources available with heterogenicity in representation from different species and pathovars. Unfortunately, the wealth of data is extremely biased and restricted to a few Xanthomonas pathogens that infect economically important plants, while those reported to infect the most diverse plants remain neglected. In the present study, we report the first complete genome sequence of Xanthomonas citri pv. durantae that was reported to infect Duranta repens L. or golden dewdrop, a hedge plant of ornamental importance native to the American region. Phylogenomic analysis with its closest relatives placed it amongst X. citri pv. citri A* pathotype strains and further comparative studies revealed various large unique genomic regions of chromosomal origin. The association of integrative and conjugative elements and prophages with unique genomic regions suggests the role of mobilome in genome dynamics. A large number of IS elements and transcription activator-like effectors encoding genes on each of the four plasmids indicate the further scope of diversification in Xanthomonas .
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OBJECTIF: Fournir des directives claires et concises pour le diagnostic et la prise en charge de la rupture prématurée des membranes avant terme (RPMAT). POPULATION CIBLE: Toute patiente manifestant une rupture prématurée des membranes avant 37 semaines d'aménorrhée. BéNéFICES, RISQUES ET COûTS: La présente directive clinique vise à fournir les premières recommandations générales canadiennes sur la prise en charge de la rupture des membranes avant terme. Elle repose sur un examen complet et à jour des données probantes sur le diagnostic de la rupture et sur la prise en charge, le bon moment et les modes d'accouchement. DONNéES PROBANTES: Des recherches ont été effectuées dans PubMed-Medline et Cochrane en 2021 en utilisant les termes suivants : preterm premature rupture of membranes, PPROM, chorioamnionitis, Nitrazine test, ferning, commercial tests, PAMG-1, IGFBP-1 test, ultrasonography, PPROM/antenatal corticosteroids, PPROM/Magnesium sulphate, PPROM/antibiotic treatment, PPROM/tocolysis, PPROM/preterm labour, PPROM/neonatal outcomes, PPROM/mortality, PPROM/outpatient/inpatient, PPROM/cerclage, previable PPROM. Les articles retenus sont des essais cliniques randomisés, des méta-analyses, des revues systématiques, des directives cliniques et des études observationnelles. D'autres publications pertinentes ont été sélectionnées à partir des notices bibliographiques de ces articles. Seuls les articles en anglais ont été examinés. MéTHODES DE VALIDATION: Les auteurs ont évalué la qualité des données probantes et la force des recommandations en utilisant le cadre méthodologique GRADE (Grading of Recommendations, Assessment, Development, and Evaluation). Voir l'annexe A (tableau A1 pour les définitions et tableau A2 pour l'interprétation des recommandations fortes et faibles). PROFESSIONNELS CONCERNéS: Tous les fournisseurs de soins de santé prénatale ou périnatale. DÉCLARATIONS SOMMAIRES: RECOMMANDATIONS.
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Rotura Prematura de Membranas Fetales , Recién Nacido , Embarazo , Femenino , HumanosRESUMEN
Xanthomonas species colonize many host plants and cause huge losses worldwide. Transcription activator-like effectors (TALEs) are secreted by Xanthomonas and translocated into host cells to manipulate the expression of target genes, especially by Xanthomonas oryzae pv. oryzae and Xanthomonas oryzae pv. oryzicola, which cause bacterial blight and bacterial leaf streak, respectively, in rice. In this review, we summarize the progress of studies on the interaction between Xanthomonas and hosts, covering both rice and other plants. TALEs are not only key factors that make plants susceptible but are also essential components of plant resistance. Characterization of TALEs and TALE-like proteins has improved our understanding of TALE evolution and promoted the development of gene editing tools. In addition, the interactions between TALEs and hosts have also provided strategies and possibilities for genetic engineering in crop improvement.
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Interacciones Huésped-Patógeno , Oryza , Efectores Tipo Activadores de la Transcripción , Xanthomonas , Interacciones Huésped-Patógeno/genética , Oryza/metabolismo , Oryza/microbiología , Enfermedades de las Plantas/genética , Enfermedades de las Plantas/microbiología , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Efectores Tipo Activadores de la Transcripción/genética , Efectores Tipo Activadores de la Transcripción/metabolismo , Xanthomonas/genética , Xanthomonas/metabolismoRESUMEN
KEY MESSAGE: The overexpression of RXam2, a cassava NLR (nucleotide-binding leucine-rich repeat) gene, by stable transformation and gene expression induction mediated by dTALEs, reduce cassava bacterial blight symptoms. Cassava (Manihot esculenta) is a tropical root crop affected by different pathogens including Xanthomonas phaseoli pv. manihotis (Xpm), the causal agent of cassava bacterial blight (CBB). Previous studies have reported resistance to CBB as a quantitative and polygenic character. This study sought to validate the functional role of a NLR (nucleotide-binding leucine-rich repeat) associated with a QTL to Xpm strain CIO151 called RXam2. Transgenic cassava plants overexpressing RXam2 were generated and analyzed. Plants overexpressing RXam2 showed a reduction in bacterial growth to Xpm strains CIO151, 232 and 226. In addition, designer TALEs (dTALEs) were developed to specifically bind to the RXam2 promoter region. The Xpm strain transformed with dTALEs allowed the induction of the RXam2 gene expression after inoculation in cassava plants and was associated with a diminution in CBB symptoms. These findings suggest that RXam2 contributes to the understanding of the molecular basis of quantitative disease resistance.
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Manihot , Xanthomonas , Leucina , Manihot/genética , Nucleótidos , Enfermedades de las Plantas/microbiologíaRESUMEN
Contemporary American Indians suffer from disproportionately high degrees of psychiatric distress. Mental health researchers and professionals, as well as American Indian community members, have consistently associated these disproportionate rates of distress with Indigenous historical experiences of European and Euro-American colonization. This emphasis on the impact of colonization and associated historical consciousness within tribal communities has occasioned increasingly widespread professional consideration of historical trauma among Indigenous peoples. In contrast to personal experiences of a traumatic nature, the discourse of Indigenous historical trauma (IHT) weds the concepts of "historical oppression" and "psychological trauma" to explain community-wide risk for adverse mental health outcomes originating from the depredations of past colonial subjugation through intergenerational transmission of vulnerability and risk. Long before the emergence of accounts of IHT, however, many American Indian communities prized a markedly different form of narrative: the coup tale. By way of illustration, I explore various historical functions of this speech genre by focusing on Aaniiih-Gros Ventre war narratives, including their role in conveying vitality or life. By virtue of their recognition and celebration of agency, mastery, and vitality, Aaniiih war stories functioned as the discursive antithesis of IHT. Through comparative consideration of the coup tale and the trauma narrative, I propose an alternative framework for cultivating Indigenous community "survivance" rather than vulnerability based on these divergent discursive practices.