Anatomical, histological, and scanning electron microscopic features of the esophagus and crop in young and adult domestic pigeons (Columba livia Domestica).

BACKGROUND: Pigeons (Columba livia) are mainly raised as a source of animal protein, racing, leisure and as an experimental animal. The present study investigated the morphology of the esophagus in the young and adult domestic pigeon, Columba livia domestica. METHODS: Ten young and ten adult, normal, and healthy pigeons were collected from the local breeders. Samples from different parts of esophagus and crop were examined grossly, by stereomicroscopy, scanning and light microscopy. RESULTS: The esophagus consisted of a long cervical part, a crop, and a short thoracic part. The crop was represented by a thin-walled outpouching with two lateral diverticula. The mucosa presented wavy fine folds in the cervical esophagus, irregular folds in the lateral diverticula giving it a corrugated appearance, and prominent longitudinal folds with several gland openings in the middle and lower parts of the crop, as well as in the thoracic esophagus. The density of gland openings was higher in adult pigeons than that in young pigeons. The mucosa of the esophagus was lined by non-keratinized stratified squamous epithelium. The shape, height, and branching of the mucosal folds differed between young and adult pigeons. Mucous-secreting alveoli were detected in the middle part of the crop as well as in the thoracic esophagus, but not in the cervical esophagus or lateral diverticula of the crop. CONCLUSION: The variations between the young and adult pigeons suggest a functional adaptation of adult pigeons to their diet compared to young pigeons.

Phylogenetic position of the pigeon mite, Ornithonyssus sylviarum, with amplification of its immunogenetic biomarkers in Egypt.

Ornithonyssus sylviarum (O. sylviarum) is an obligatory, blood-sucking ectoparasite widely distributed among poultry and other mammals, causing significant economic losses. This study represented the first report of molecular genotypic identification of O. sylviarum from pigeons, Columba livia domestica, in Egypt. PCR and sequencing of the 28S rRNA gene were conducted. The resulting mite sequences were subjected to BLAST analysis, revealing 90-100% similarity to O. sylviarum in all tested samples. The sequences were deposited in GenBank under the accession numbers PP049086 and PP033720. A phylogenetic tree was constructed to compare the obtained species with related species worldwide. Additionally, infected pigeons showed increased expression of IL-1, IL-10, IFN-γ, and TGF-ß3 genes and elevated serum levels of stress biomarkers. The increased level of these cytokines indicates there was a disturbance in the immune status of the infected host with parasite compared with control healthy ones. This increases the susceptibility to infection with other pathogens.

Metabolite profiling and molecular characterization of NBAIR BSWG1: A potential strain of Bacillus subtilis against Fusarium oxysporium f. sp. udum.

To address the fungal wilt of pigeon pea caused by Fusarium oxysporium f. sp. udum, farmers currently rely on chemical fungicides, despite their harmful effects. However, there is a growing need for safer alternatives like green pesticides. Bacterial biocontrol agents and their derivatives serve as potential green pesticides in the management of plant pathogens. In the present study, we aimed to identify indigenous Bacillus subtilis strains effective against F. oxysporium f. sp. udum. We used PCR and MALDI-TOF analysis to identify the active components responsible for the efficiency of efficient strain. Biochemical studies of cell-free extracts extracted from B. subtilis strains demonstrated the highest biosurfactant activity in NBAIR BSWG1, with an oil displacement of 2 cm and an emulsification index of 60 %. Molecular characterization confirmed the presence of surfactin, fengycin, and iturin coding genes in the B. subtilis strains, among them, NBAIR BSWG1 showed the highest number of lipopeptide-producing genes. Meanwhile, NBAIR BSWG1 showed inhibition of 79.84 % against F. oxysporium f. sp. udum using cell-free extract. Further metabolite profiling of NBAIR BSWG1 using MALDI-TOF analysis further confirmed surfactin, fengycin, and iturin in the purified cell-free extract of NBAIR BSWG1. Two peaks with m/z of 923.77 and 1149.92 were identified as novel lipopeptide compounds which need further characterization. The present study identified NBAIR BSWG1 as an efficient bacterial strain for the inhibition of F. oxysporium f. sp. udum and its antifungal properties are mainly due to the production of cyclic lipopeptides.

Pathological and immunohistochemical studies of lymphoid leukosis in pigeons in Egypt.

Background: Pigeon leukosis is primarily caused by avian leukosis virus subgroup A (ALV-A). It infects and transforms lymphoid cells, leading to the development of tumors in various lymphoid tissues and other organs especially the liver. Aim: This study was conducted to diagnose lymphoid leukosis in a naturally infected pigeon flock in Egypt. Methods: Tissue specimens from the liver, spleen, thymus, kidney, lung, proventriculus, gizzard, intestine, pancreas, heart, pectoral muscle, ovary, and testes were collected from infected birds for pathological and immunohistochemical examinations. Results: Clinical signs were generally nonspecific and comprised weakness, dehydration, and emaciation. Gross lesions were mostly in the liver and spleen, in the form of minute white nodules scattered on the liver surface. Microscopic examination of the liver, spleen, and kidneys showed masses of uniform sizes and the presence of differentiated lymphoid cells. These cells appeared as large mononuclear cells with poorly defined cell membranes. Immunohistochemical investigation exhibited that the ALV-A positive indicators were chiefly accessible in the liver, ovary, spleen, and kidney. Conclusion: Lymphoid leukosis in pigeons could be provisionally diagnosed by a pathological picture of characteristic tumors and confirmed by immunoreactivity of viral antigens in different tissues.

Antimicrobial Susceptibility and Molecular Identification of Antibiotic Resistance Enteric Bacteria Isolated From Pigeon Feces in the City of Jeddah, Saudi Arabia.

Background Due to their potential to carry a wide range of bacteria, pigeon feces may contribute to the spreading of infectious diseases in urban settings.  Objective This study analyzed the presence of enteric bacteria from pigeon feces in Jeddah and their antimicrobial susceptibility and described the molecular characteristics of the carbapenem resistance genes it produced. Method Two hundred twenty-five pigeon feces specimens were collected from eight parks in Jeddah. Conventional microbiology techniques were employed to identify the isolated bacteria, and the automated Vitek2® system (bioMérieux, Marcy-l'Étoile, Lyon, France) provided additional confirmation. Kirby-Bauer disk diffusion method was utilized to screen for antimicrobial resistance. Only 50 antibiotic-resistance isolates further underwent molecular diagnosis for testing groups of carbapenems-encoding genes (blaNDM, blaSIM, and blaAIM), using multiplex polymerase chain reaction (PCR).  Result Of the 50 antibiotic-resistant isolates, 28% (14/50) were Klebsiella pneumoniae, 24% (12/50) were Enterobacter cloacae, and 48% (24/50) were Escherichia coli. Ninety percent (90%) of the isolates showed resistance to cefuroxime, 56% to gentamicin, 52% to amoxicillin/clavulanic acid, and 100% to meropenem. NDM beta-lactamase was the most often discovered gene (26%) and was followed by AIM beta-lactamase (5%) Conclusion According to this study, there may be a chance for resistant K. pneumoniae, E. cloacae, and E. coli to spread amongst several hosts within the same area. Consequently, to prevent the continued occurrence and dissemination of resistant strains among other hosts in the same location, it is essential to monitor the AMR (antimicrobial resistance) of E. coli, E. cloacae, and K. pneumoniae from pigeons.

Comparison of two polar equations in describing the geometries of domestic pigeon (Columba livia domestica) eggs.

Two-dimensional (2D) egg-shape equations are potent mathematical tools, facilitating the description of avian egg geometries in their applied mathematical modelling and poultry science implementations. They aid in the precise quantification of avian egg sizes, including traits such as volume (V) and surface area (S). Despite their potential, however, polar coordinate egg-shape equations have received relatively little attention for practical applications in oomorphology. This may be attributed to their complex model structure and the absence of explicit geometric interpretations for the equation parameters. In the present study, 2 distinct polar equations, namely the Carter-Morley Jones equation (CMJE) and simplified Gielis equation (SGE), were used to fit the profile geometries of 415 domestic pigeon (Columba livia domestica) eggs based on nonlinear least squares regression methods. The adequacy of goodness-of-fit for each nonlinear egg-shape equation was evaluated through the adjusted root-mean-square error (RMSEadj), while relative curvature measures of nonlinearity were utilized to assess the nonlinear behavior of equations. All of the RMSEadj values of the 2 polar equations were lower than 0.05, which demonstrated the validity of CMJE and SGE in depicting the shapes of C. livia egg profiles. Moreover, the 2 egg-shape equations showed good nonlinear behavior across all 415 C. livia eggs. Wilcoxon signed rank tests relative to RMSEadj values between CMJE and SGE revealed that CMJE displayed inferior fits to empirical data when compared to SGE. CMJE, however, had a better linear approximation performance than SGE at the global level. At the individual parameter level, all of the parameters of CMJE or SGE exhibited good close-to-linear behavior. This study provides an instrumental mathematical tool for the practical application of polar egg-shape equations, such as nondestructively estimating V and S of avian eggs. Additionally, it offers valuable insights into assessing nonlinear regression models for accurately describing the geometries of 2D egg profiles.

Overexpression of rice lectin receptor-like kinase, OsLec-RLK, confers salinity stress tolerance and increases seed yield in pigeon pea (Cajanus cajan (L.) Millsp.).

KEY MESSAGE: OsLec-RLK overexpression enhances cell signalling and salt stress tolerance in pigeon pea, enhancing seed yield and harvest index and thus, enabling marginal lands to increase food and nutritional security. Lectin Receptor-like kinases (Lec-RLKs) are highly effective cell signaling molecules that counteract various stresses, including salt stress. We engineered pigeon pea by overexpressing OsLec-RLK gene for enhancing salt tolerance. The OsLec-RLK overexpression lines demonstrated superior performance under salt stress, from vegetative to reproductive phase, compared to wild types (WT). The overexpression lines had significantly higher K+/Na+ ratio than WT exposed to 100 mM NaCl. Under salt stress, transgenic lines showed higher levels of chlorophyll, proline, total soluble sugars, relative water content, and peroxidase and catalase activity than WT plants. Membrane injury index and lipid peroxidation were significantly reduced in transgenic lines. Analysis of phenological and yield attributes confirmed that the OsLec-RLK pigeon pea lines maintain plant vigor, with 10.34-fold increase in seed yield (per plant) and 4-5-fold increase in harvest index of overexpression lines, compared to wild type. Meanwhile, the overexpression of OsLec-RLK up-regulated the expression levels of histone deacetylase1, acyl CoA, ascorbate peroxidase, peroxidase, glutathione reductase and catalase, which were involved in the K+/Na+ homeostasis pathway. This study showed the potential of OsLec-RLK gene for increasing crop productivity and yields under salt stress and enabling the crops to be grown on marginal lands for increasing food and nutritional security.

Zoonotic potential of urban wildlife faeces, assessed through metabarcoding.

Monitoring zoonoses in urban environments is of great relevance, where the incidence of certain pathogens may be higher and where population density makes the spread of any contagious disease more likely. In this study we applied a metabarcoding approach to study potentially zoonotic pathogens in faecal samples of 9 urban vertebrate species. We applied this methodology with two objectives. Firstly, to obtain information on potential pathogens present in the urban fauna of a large European city (Madrid, Spain) and to determine which are their main reservoirs. In addition, we tested for differences in the prevalence of these potential pathogens between urban and rural European rabbits, used as ubiquitous species. Additionally, based on the results obtained, we evaluated the effectiveness of metabarcoding as a tool for monitoring potential pathogen. Our results revealed the presence of potentially zoonotic bacterial genera in all studied host species, 10 of these genera with zoonotic species of mandatory monitoring in the European Union. Based on these results, urban birds (especially house sparrows and pigeons) and bats are the species posing the greatest potential risk, with Campylobacter and Listeria genera in birds and of Chlamydia and Vibrio cholerae in bats as most relevant pathogens. This information highlights the risk associated with fresh faeces from urban wildlife. In addition, we detected Campylobacter in >50 % of the urban rabbit samples, while we only detected it in 11 % of the rural rabbit samples. We found that urban rabbits have a higher prevalence of some pathogens relative to rural rabbits, which could indicate increased risk of pathogen transmission to humans. Finally, our results showed that metabarcoding can be an useful tool to quickly obtain a first screening of potentially zoonotic organisms, necessary information to target the monitoring efforts on the most relevant pathogens and host species.

A new glycoprotein from pigeon egg: Study on its structure and digestive characteristics.

Pigeon egg white (PEW) is widely recognized as a promising source of bioactive proteins, with a high degree of glycosylation. This study focused on the characterization of a novel glycoprotein extracted from PEW, known as ovalbumin-related protein Y (OVAY). Our investigation included an analysis of the N-glycan and protein structures of OVAY, as well as an examination of simulated gastrointestinal digestive products and the transmembrane transport mechanism of OVAY-digested peptides. The results revealed that OVAY contains two glycosylation sites (Asn 62, 215) and consists of 30 N-linked glycoforms, with the top three glycans being N6H3, N6H7S1, and N6H5. Additionally, OVAY is rich in Gal and sialic acid and exhibits a rod-like molecular structure. Furthermore, it was found that OVAY demonstrates resistance to gastric digestion, with its digested peptides primarily transported via PepT1 and endocytosis. This study provides insight into the glycoprotein structure of OVAY and elucidates its physiological activity, providing a theoretical reference for the development of a novel sialate-rich protein.

Sonification of Genomic Data to Represent Genetic Load in Zoo Populations.

Maintaining a diverse gene pool is important in the captive management of zoo populations, especially in endangered species such as the pink pigeon (Nesoenas mayeri). However, due to the limited number of breeding individuals and relaxed natural selection, the loss of variation and accumulation of harmful variants is inevitable. Inbreeding results in a loss of fitness (i.e., inbreeding depression), principally because related parents are more likely to transmit a copy of the same recessive deleterious genetic variant to their offspring. Genomics-informed captive breeding can manage harmful variants by artificial selection, reducing the genetic load by avoiding the inheritance of two copies of the same harmful variant. To explain this concept in an interactive way to zoo visitors, we developed a sonification game to represent the fitness impacts of harmful variants by detuning notes in a familiar musical melody (i.e., Beethoven's Für Elise). Conceptually, zoo visitors play a game aiming to create the most optimal pink pigeon offspring in terms of inbreeding depression. They select virtual crosses between pink pigeon individuals and listen for the detuning of the melody, which represents the realised load of the resultant offspring. Here we present the sonification algorithm and the results of an online survey to see whether participants could identify the most and least optimal offspring from three potential pink pigeon offspring. Of our 98 respondents, 85 (86.7%) correctly identified the least optimal offspring, 73 (74.5%) correctly identified the most optimal, and 62 (63.3%) identified both the most and least optimal offspring using only the sonification.

[Three-dimensional positioning and trajectory tracking of pigeons in large indoor spaces].

Animal localization and trajectory tracking are of great value for the study of brain spatial cognition and navigation neural mechanisms. However, traditional optical lens video positioning techniques are limited in their scope due to factors such as camera perspective. For pigeons with excellent spatial cognition and navigation abilities, based on the beacon positioning technology, a three-dimensional (3D) trajectory positioning and tracking method suitable for large indoor spaces was proposed, and the corresponding positioning principle and hardware structure were provided. The results of in vitro and in vivo experiments showed that the system could achieve centimeter-level positioning and trajectory tracking of pigeons in a space of 360 cm × 200 cm × 245 cm. Compared with traditional optical lens video positioning techniques, this system has the advantages of large space, high precision, and high response speed. It not only helps to study the neural mechanisms of pigeon 3D spatial cognition and navigation, but also has high reference value for trajectory tracking of other animals.

Enhanced production of health-promoting phenolic compounds using a novel endophytic fungus Talaromyces neorugulosus R-209 isolated from pigeon pea in a natural habitat by l-phenylalanine feeding.

In this study, nine endophytic fungi capable of producing multiple phenolic compounds were screened and identified from 152 fungi isolated from pigeon pea in a natural habitat (Honghe, Yunnan Province, China). Talaromyces neorugulosus R-209 exhibited the highest potential for phenolic compound production. L-phenylalanine feeding was used to enhance phenolic compound production in T. neorugulosus R-209 cultures. Under the optimal feeding conditions (l-phenylalanine dose of 0.16 g/L and feeding phase of 6 days), the yields of genistein, apigenin, biochanin A, and cajaninstilbene acid increased by 15.59-fold, 7.20-fold, 25.93-fold, and 10.30-fold over control, respectively. T. neorugulosus R-209 fed with l-phenylalanine was found to be stable in the production of phenolic compounds during ten successive subcultures. Moreover, bioactivities of extracts of T. neorugulosus R-209 cultures were significantly increased by l-phenylalanine feeding. Overall, l-phenylalanine feeding strategy made T. neorugulosus R-209 more attractive as a promising alternative source for the production of health-beneficial phenolic compounds in the nutraceutical/medicinal industries.

MtDNA genetic diversity and phylogeographic insights into giant domestic pigeon (Columba livia domestica) breeds: connections between Central Europe and the Middle East.

Humans have selectively bred domestic pigeons (Columba livia domestica) to create breeds with a diversity of shapes, colors and other attributes. Since Darwin, the domestic pigeon has always been a popular model species for scientific research because of its richness of form, colouration and behaviour. It is believed that the world's squab pigeon industry uses breeds and hybrids from the Mediterranean region. An exception is the indigenous giant pigeon breeds of the Carpathian Basin, whose origin is not known. Therefore, our aims were 1) to understand the phylogenetic relationships of giant pigeons, which sheds light on the origin of Hungarian breeds and their relationship to the Mediterranean giant pigeon breed group; 2) to contribute molecular genetic data to the genealogy of 2 Iraqi pigeon breeds close to the pigeon domestication center, including the culturally important Iraqi Red Pigeon, and 3) to compare the genetic diversity of European and Middle Eastern domestic pigeon populations and to draw conclusions on the phylogenetic relationships between pigeon breeds and molecular clues to their different breeding practices of both regions. A 655-bp-long sequence of the cytochrome oxidase 1 (COI) region of the mitochondrial DNA was studied in a total of 276 pigeons (19 breeds). A total of 27 haplotypes were found, of which 22 were unique. The highest genetic diversity was found in the Carpathian Basin, and the lowest in the Iraqi region. STRUCTURE analysis revealed low structurality, K=3 was the most likely. The majority of the samples belong to the most ancient haplotype H_2=219, however the Jacobin pigeon is on a very separate evolutionary branch with a large number of mutations. None of the 19 breeds investigated in this study have been previously studied in phylogenetics, and most of these breeds have potential as squab pigeons, and have good meat forms for utilization, therefore the results of this study may also be of help to the squab pigeon industry.

Fine-scale tracking reveals visual field use for predator detection and escape in collective foraging of pigeon flocks.

During collective vigilance, it is commonly assumed that individual animals compromise their feeding time to be vigilant against predators, benefiting the entire group. One notable issue with this assumption concerns the unclear nature of predator 'detection', particularly in terms of vision. It remains uncertain how a vigilant individual utilizes its high-acuity vision (such as the fovea) to detect a predator cue and subsequently guide individual and collective escape responses. Using fine-scale motion-capture technologies, we tracked the head and body orientations of pigeons (hence reconstructed their visual fields and foveal projections) foraging in a flock during simulated predator attacks. Pigeons used their fovea to inspect predator cues. Earlier foveation on a predator cue was linked to preceding behaviors related to vigilance and feeding, such as head-up or down positions, head-scanning, and food-pecking. Moreover, earlier foveation predicted earlier evasion flights at both the individual and collective levels. However, we also found that relatively long delay between their foveation and escape responses in individuals obscured the relationship between these two responses. While our results largely support the existing assumptions about vigilance, they also underscore the importance of considering vision and addressing the disparity between detection and escape responses in future research.

Most animals have to compromise between spending time foraging for food and other resources and keeping careful watch for approaching predators or other threats. Many are thought to address this trade-off by living in a group where they rely on the vigilance of others to free up more time for foraging. If one individual animal detects a threat, they alert the whole group so that every individual can respond. However, it remains unclear how individuals use vision to detect a threat and how they communicate the threat to the rest of the group. Pigeons are a useful animal model to address this question because they tend to live in groups and their vision is well understood. A pit at the back of their eye called the fovea is responsible for building clear, detailed images of the centre of the field of vision. When pigeons attend to something of interest, they typically direct their gaze by moving their whole head instead of moving their eyes, making head orientation a good proxy for researchers to track where they are looking. To better understand how pigeons detect potential threats and communicate them to the rest of the flock, Delacoux and Kano used motion capture technology to track the head movements of groups of pigeons. To encourage the pigeons to forage, grain was scattered in the centre of an enclosed room. A plastic sparrowhawk (representing a potential predator) would then emerge and move across the room before disappearing again. Analysis of the imaging data revealed that pigeons use their fovea to spot predators. Individuals that were looking around before the potential predator emerged directed their fovea towards it more quickly than pigeons that were eating. These pigeons also took flight more quickly, and this likely triggered the rest of the group to follow. Due to improvements in the tracking technologies, these findings may help scientists understand in finer detail how animals in a group detect and respond to threats and other cues in their environment. Therefore, the experimental approach used by Delacoux and Kano could also be used to investigate how information is passed among groups of other animal species.

Early weaning damages the intestinal epithelial barrier of squabs through toll-like receptor signaling pathways.

Stress damage caused by early weaning and its possible mechanism have been studied mainly in young mammals, but rarely in altrices, especially in squabs. The study aimed to investigate the possible molecular mechanism of intestinal epithelial barrier damage caused by early weaning in squabs through determining the intestinal permeability, the ultrastructure of villous epithelium, the contents of ileal cytokines, and the protein relative expression of tight-junction proteins, TLRs and their mediated key factors in inflammatory signaling pathways. A total of 192 newly hatched squabs were randomly divided into 2 groups, 1 group was weaned and fed artificial pigeon milk from d 7, and the other group continued to be fed by the parent pigeons. The ileal mucosa and serum of 8 replicates were collected at 1, 4, 7, 10, and 14 d after weaning. The results indicated that early weaning could reduce the growth performance of squabs and damage the intestinal epithelial barrier, which is characterized by down-regulating the protein expression of claudin-1/3, up-regulating the protein expression of claudin-2, promoting the secretion of pro-inflammatory factors, inhibiting the secretion of anti-inflammatory factors, and increasing the permeability of the intestinal barrier. The specific mechanism of stress damage might be the activation of TLR2/4-MyD88-ERK/JNK inflammatory signaling pathway leading to the increase levels of IL-6 and TNF-α.

Research Note: Study on the in-situ preservation of pigeons based on the level of endangerment of genetic resources.

The large-scale and intensive development of the meat pigeon breeding industry have resulted in the replacement of a large number of low-performance local breeds by a few breeds with excellent production performance. However, due to the characteristics of pigeon species that are monogamous, for which the W chromosome cannot be recovered and for which semen cannot be cryopreserved, the preservation of pigeon species is still mainly based on in-situ preservation. In this study, pigeons were classified into 6 classes of endangerment based on the criteria of the 100-year inbreeding coefficient of poultry populations in the "Assessment of Endangered Poultry Genetic Resources" (NY/T 2996-2016). The results show that when the generation interval was 1.5 yr, the number of ideal populations with the same gene frequency variance or the same heterozygosity decay rate of pigeons in class 1 to 5 was ≤149, 150 to 204, 205 to 316, 317 to 649 and ≥650. In random-reserved breeding, when the generation interval was 1.5 yr, the number of male (female) pigeons corresponding to class 1 to 5 was ≤74, 75 to 102, 103 to 157, 158 to 324 and ≥325. In family-equal-reserved breeding, when the generation interval was 1.5 yr, the number of male (female) pigeons corresponding to class 1 to 5 was ≤36, 37 to 50, 51 to 78, 79 to 162 and ≥163. When the generation interval was 1.5 yr, the inbreeding increments corresponding to class 1 to 5 were ≥0.00335, 0.00244 to 0.00334, 0.00159 to 0.00243, 0.00078 to 0.00158 and ≤0.00077; with the same population size, the inbreeding coefficient and inbreeding increment decreased with the increase of generation interval; the population effective content, inbreeding coefficient and inbreeding increment of family-equal-reserved pigeons were lower than those of random-reserved pigeons. The results of this study have certain reference value for analyzing the status quo of local and endangered species, constructing live gene banks and breeding farms of poultry genetic resources, and rescuing endangered species.

Medetomidine-vatinoxan-midazolam provides similar sedation depth with reduced bradycardia compared to dexmedetomidine-midazolam in pigeons (Columba livia domestica).

OBJECTIVE: To determine if sedation with medetomidine-vatinoxan (Zenalpha; Dechra Veterinary Products) and midazolam (Alvogen) (ZM) would cause less cardiovascular depression and maintain similar depth and duration of sedation in pigeons (Columba livia domestica) compared to dexmedetomidine and midazolam (DM). METHODS: In a blinded crossover study, 15 healthy adult domestic pigeons were sedated IM with either dexmedetomidine (0.08 mg/kg) and midazolam (2 mg/kg) or medetomidine (0.16 mg/kg), vatinoxan (3.2 mg/kg), and midazolam (2 mg/kg) from November through December 2023. Each subject was monitored for 60 minutes, then the sedation was reversed with atipamezole (0.8 mg/kg) and flumazenil (0.1 mg/kg) as needed. Sedation scores, heart rates, and respiratory rates were compared. RESULTS: There was no significant difference in the peak sedation score between DM and ZM groups, with both exhibiting median scores of 4 (heavy sedation). Mean heart rate was significantly higher for ZM than DM at 5, 10, 20, 30, 45, 60, and 65 minutes postinjection. Bradycardia occurred in both groups at 5 and 10 minutes postinjection and persisted for DM until reversal with atipamezole. Arrhythmias were auscultated in both groups. Bradypnea was not observed in either group, and all birds resumed normal behavior following recovery and the following day. CONCLUSIONS: Medetomidine-vatinoxan-midazolam provides a similar depth of sedation to DM but with less incidence of bradycardia. Further study is needed to determine the clinical applicability of this sedative in birds. CLINICAL RELEVANCE: Medetomidine-vatinoxan may be considered for short-term sedation and restraint in cardiovascularly stable pigeons.

First microscopic, pathological, epidemiological, and molecular investigation of Leucocytozoon (Apicomplexa: Haemosporida) parasites in Egyptian pigeons.

Introduction: Leucocytozoon is an intracellular blood parasite that affects various bird species globally and is transmitted by blackfly vectors. This parasite is responsible for leucocytozoonosis, a disease that results in significant economic losses due to reduced meat and egg production. There is limited knowledge about the epidemiological pattern of leucocytozoonosis and its causative species in Egypt, particularly in pigeons. Methods: The current study involved the collection of 203 blood samples from domestic pigeons from various household breeders and local markets across Qena Province, Upper Egypt. Samples were initially examined for potential Leucocytozoon infection using blood smears, followed by an evaluation of associated risk factors. Molecular identification of the parasite in selected samples (n = 11), which had initially tested positive via blood smears, was further refined through nested PCR and sequence analysis of the mitochondrial cytochrome b gene to ascertain the Leucocytozoon species present. Additionally, histopathological examination of the liver, spleen, and pancreas was conducted on animals that tested positive by blood smears. Results: Interestingly, 26 out of 203 samples (12.08%) had confirmed Leucocytozoon infections based on microscopic analysis. Additionally, all 11 samples that initially tested positive via blood smears were confirmed positive through nested PCR analysis, and their sequencing revealed the presence of Leucocytozoon sabrazesi, marking the first report of this parasite in Egypt. The study into potential risk factors unveiled the prevalence of Leucocytozoon spp. seems host gender-dependent, with males exhibiting a significantly higher infection rate (33.33%). Additionally, adult birds demonstrated a significantly higher infection prevalence than squabs, suggesting an age-dependent trend in prevalence. Seasonality played a significant role, with the highest occurrence observed during summer (37.25%). Histopathological examination revealed the presence of numerous megaloschizonts accompanied by lymphocytic infiltration and multiple focal areas of ischemic necrosis. Conclusion: To our knowledge, this is the first study to shed light on the epidemiological characteristics and molecular characterization of leucocytozoonosis in pigeons in Egypt. Further research endeavors are warranted to curb the resurgence of Leucocytozoon parasites in other avian species across Egypt, thereby refining the epidemiological understanding of the disease for more effective control and prevention measures.

Balanced anesthesia in pigeons (Columba livia): a protocol that ensures stable vital parameters and feasibility during long surgeries in cognitive neuroscience.

In neuroscience, numerous experimental procedures in animal models require surgical interventions, such as the implantation of recording electrodes or cannulas before main experiments. These surgeries can take several hours and should rely on principles that are common in the field of research and medicine. Considering the characteristics of the avian respiratory physiology, the development of a safe and replicable protocol for birds is necessary to minimize side effects of anesthetic agents, circumvent technical limitations due to the insufficient availability of patient monitoring, and to maintain stable intraoperative anesthesia. Through the consistent and responsible implementation of the three R principle of animal welfare in science ("Replace, Reduce, Refine"), we aimed to optimize experimental methods to minimize the burden on pigeons (Columba livia) during surgical procedures. Here, surgeries were conducted under balanced anesthesia and perioperative monitoring of heart rate, oxygen saturation, body temperature, and the reflex state. The protocol we developed is based on the combination of injectable and inhalative anesthetic drugs [ketamine, xylazine, and isoflurane, supported by the application of an opiate for analgesia (e.g., butorphanol, buprenorphine)]. The combination of ketamine and xylazine with a pain killer is established in veterinary medicine across a vast variety of species. Practicability was verified by survival of the animals, fast and smooth recovery quantified by clinical examination, sufficiency, and stability of anesthesia. Independent of painful stimuli like incision or drilling, or duration of surgery, vital parameters were within known physiological ranges for pigeons. Our approach provides a safe and conservative protocol for surgeries of extended duration for scientific applications as well as for veterinary medicine in pigeons which can be adapted to other bird species.

Integration of simulated annealing into pigeon inspired optimizer algorithm for feature selection in network intrusion detection systems.

In the context of the 5G network, the proliferation of access devices results in heightened network traffic and shifts in traffic patterns, and network intrusion detection faces greater challenges. A feature selection algorithm is proposed for network intrusion detection systems that uses an improved binary pigeon-inspired optimizer (SABPIO) algorithm to tackle the challenges posed by the high dimensionality and complexity of network traffic, resulting in complex models, reduced accuracy, and longer detection times. First, the raw dataset is pre-processed by uniquely one-hot encoded and standardized. Next, feature selection is performed using SABPIO, which employs simulated annealing and the population decay factor to identify the most relevant subset of features for subsequent review and evaluation. Finally, the selected subset of features is fed into decision trees and random forest classifiers to evaluate the effectiveness of SABPIO. The proposed algorithm has been validated through experimentation on three publicly available datasets: UNSW-NB15, NLS-KDD, and CIC-IDS-2017. The experimental findings demonstrate that SABPIO identifies the most indicative subset of features through rational computation. This method significantly abbreviates the system's training duration, enhances detection rates, and compared to the use of all features, minimally reduces the training and testing times by factors of 3.2 and 0.3, respectively. Furthermore, it enhances the F1-score of the feature subset selected by CPIO and Boost algorithms when compared to CPIO and XGBoost, resulting in improvements ranging from 1.21% to 2.19%, and 1.79% to 4.52%.