Changes in free amino acid and protein polymerization in wheat caryopsis and endosperm during filling after shading.

Over the past several decades, a decreasing trend in solar radiation has been observed during the wheat growing season. The effects of shade stress on grain yield formation have been extensively studied. However, little information on shade stress's effects on protein formation warrants further investigation. Two wheat cultivars were grown under three treatments, no shade as the control group (CK), shading from the joint to the anthesis stage (S1), and shading from the joint to the mature stage (S2), to investigate the effects of shade stress on the free amino acids of the caryopsis and endosperm and protein accumulation during grain filling. The dry mass of caryopsis and endosperm was significantly decreased under shade stress, whereas Glu, Ser, Ala, and Asp and protein relative content increased during grain filling. The observed increases in total protein in S1 and S2 were attributed to the increases in the SDS-isoluble and SDS-soluble protein extracts, respectively. S1 improved polymer protein formation, but S2 delayed the conversion of albumins and globulins into monomeric and polymeric proteins. Moreover, shade stress increased the proportion of SDS-unextractable polymeric protein, which represented an increase in the degree of protein polymerization. The polymerization of protein interrelations between protein components and accumulation in caryopsis and endosperm provided novel insights into wheat quality formation under shade stress.

Relationship between the baking quality of wheat (Triticum aestivum L.) and the protein composition and structure after shading.

Although wheat (Triticum aestivum L.) grain protein content is increased by shade stress, the relationship between the baking quality of wheat flour and protein composition and structure remains unclear. Here, we investigated the effects of shade stress on wheat flour protein composition and structure. The contents of the flour protein, α/ß-gliadins and disulfide and hydrogen bonds were significantly increased by shade stress. Glutenins, UPP%, and ß-sheet contents also increased, whereas that of α-helices decreased. Spearman correlations revealed that the flour protein content, Glu:Gli ratio, and disulfide, hydrogen, and ionic bonds can predict the specific volume and number of crumb cells in bread, whereas α/ß-gliadins content can predict the crumb cell wall thickness and diameter of bread. Under shade stress, variations in protein composition and structure help increase the specific volume and crumb cells number and decrease crumb cell wall thickness and diameter of bread, ultimately leading to improved baking quality.

Alleviation of Shade Stress in Chinese Yew (Taxus chinensis) Seedlings with 5-Aminolevulinic Acid (ALA).

5-aminolevulinic acid (ALA) is a novel regulator that can promote plant growth, nitrogen uptake, and abiotic stress tolerance. Its underlying mechanisms, however, have not been fully investigated. In this study, the effects of ALA on morphology, photosynthesis, antioxidant systems, and secondary metabolites in two cultivars of 5-year-old Chinese yew (Taxus chinensis) seedlings, 'Taihang' and 'Fujian', were examined under shade stress (30% light for 30 days) using different doses of ALA (0, 30, and 60 mg/L). The findings from our study show that shade stress significantly reduced plant height, stem thickness, and crown width and increased malondialdehyde (MDA) levels. However, the application of 30 mg/L ALA effectively mitigated these effects, which further induced the activity of antioxidant enzymes under shade stress, resulting in the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) being increased by 10%, 16.4%, and 42.1%, and 19.8%, 20.1%, and 42% in 'Taihang' and 'Fujian', respectively. It also promoted their role in the absorption, conversion, and efficient use of light energy. Additionally, the use of 30 mg/L ALA caused a significant increase in the concentration of secondary metabolites, including polysaccharide (PC), carotenoid (CR), and flavonoids (FA), with increases of up to 46.1%, 13.4%, and 35.6% and 33.5%, 7.5%, and 57.5% in both yew cultivars, respectively, contributing to nutrient uptake. With ALA treatment, the yew seedlings showed higher chlorophyll (total chlorophyll, chlorophyll a and b) levels and photosynthesis rates than the seedlings that received the shade treatment alone. To conclude, the application of 30 mg/L ALA alleviated shade stress in yew seedlings by maintaining redox balance, protecting the photorespiratory system, and increasing organic metabolites, thus increasing the number of new branches and shoots and significantly promoting the growth of the seedlings. Spraying with ALA may be a sustainable strategy to improve the shade-resistant defense system of yew. As these findings increase our understanding of this shade stress response, they may have considerable implications for the domestication and cultivation of yew.

Genome-Wide Identification of SMXL Gene Family in Soybean and Expression Analysis of GmSMXLs under Shade Stress.

SMXL6,7,8 are important target proteins in strigolactone (SL) signal pathway, which negatively regulate the reception and response of SL signal, and play an important role in regulating plant branching. However, there is a relative lack of research on soybean SMXL gene family. In this study, 31 soybean SMXL genes were identified by phylogenetic analysis and divided into three groups. Based on the analysis of GmSMXL gene's structure and motif composition, it was found that the GmSMXL members in the same group were similar. The results of cis-element analysis showed that GmSMXL genes may regulate the growth and development of soybean by responding to hormones and environment. Based on the tissue specificity analysis and GR24 treatment, the results showed that four GmSMXLs in G1 group were predominantly expressed in stems, axillary buds and leaves and involved in SL signal pathway. Finally, under shading stress, the expression of four genes in G1 group was slightly different in different varieties, which may be the reason for the difference in branching ability of different varieties under shading stress. We have systematically studied the SMXL gene family in soybean, which may lay a foundation for the study of the function of GmSMXL gene in the future.

Foliar Application of NH4 +/NO3 - Ratios Enhance the Lodging Resistance of Soybean Stem by Regulating the Physiological and Biochemical Mechanisms Under Shade Conditions.

Shading is one of the most chronic restrains which can lead to the lodging of intercropped plants. In order to increase the soybean stem lodging resistance, a 2-year field trial was conducted to evaluate the impact of different ratios and concentrations of NH4 +/NO3 - on the morpho-physiological and biochemical characteristics of soybean stem under shade conditions. The total 5 ratios of NH4 +/NO3 - were applied as follows: T0 = 0/0 (control), T1 = 0/100 (higher ratio), T2 = 25/75 (optimum), T3 = 50/50 (optimum), and T4 = 75/25 (higher ratio) as a nitrogen source. Our findings displayed that the T2 (25/75) and T3 (50/50) treatments alleviated the shading stress by improving the photosynthetic activity, biomass accumulation, carbohydrates contents, and lignin related enzymes (POD, CAD, and 4Cl) which led to improvement in stem lodging resistance. The correlation analysis (p ≤ 0.05, p ≤ 0.01) revealed the strong relationship between lodging resistance index and stem diameter, stem strength, lignin content, photosynthesis, and lignin related enzymes (POD, CAD, and 4CL) evidencing the strong contribution of lignin and its related enzymes in the improvement of lodging resistance of soybean stem under shade conditions. Collectively, we concluded that optimum NH4 +/NO3 - ratios (T2 and T3) can boost up the lodging resistance of soybean stem under shade stress.

Foliar application of silicon improves growth of soybean by enhancing carbon metabolism under shading conditions.

An experiment was set up to investigate physiological responses of soybeans to silicon (Si) under normal light and shade conditions. Two soybean varieties, Nandou 12 (shade resistant), and Nan 032-4 (shade susceptible), were tested. Our results revealed that under shading, the net assimilation rate and the plant growth were significantly reduced. However, foliar application of Si under normal light and shading significantly improved the net photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (Tr), and decreased intercellular carbon dioxide concentration (Ci). The net photosynthetic rate of Nandou 12 under normal light and shading increased by 46.4% and 33.3% respectively with Si treatment (200 mg/kg) compared to controls. Si application also enhanced chlorophyll content, soluble sugars, fresh weight, root length, root surface area, root volume, root-shoot ratio, and root dry weight under both conditions. Si application significantly increased the accumulation of some carbohydrates such as soluble sugar and sucrose in stems and leaves ensuring better stem strength under both conditions. Si application significantly increased the yield by increasing the number of effective pods per plant, the number of beans per plant and the weight of beans per plant. After Si treatment, the yield increased 24.5% under mono-cropping, and 17.41% under intercropping. Thus, Si is very effective in alleviating the stress effects of shading in intercropped soybeans by increasing the photosynthetic efficiency and lodging resistance.

Transcriptional analyses reveal the molecular mechanism governing shade tolerance in the invasive plant Solidago canadensis.

Solidago canadensis is an invasive plant that is capable of adapting to variable light conditions. To elucidate the shade tolerance mechanism in S. canadensis at the molecular level, transcriptome analyses were performed for leaves growing under natural light and three shade level conditions. Many differentially expressed genes (DEGs) were found in the comparative analysis, including those involved in photosynthesis, antioxidant, and secondary metabolism of phenol- and flavonoid-related pathways. Most genes encoding proteins involved in photosynthesis, such as photosystem I reaction center subunit (Psa), photosystem II core complex protein (Psb), and light-harvesting chlorophyll protein (Lhca and Lhcb), and reactive oxygen species (ROS) scavenging-related enzymes, such as superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), were upregulated with the shade levels. Furthermore, most of the DEGs related to secondary metabolite synthesis were also upregulated in the shade conditions. Our study indicates that S. canadensis can respond to shade stress by modulating the expression of several photosynthesis-related, free radical scavenging-related, and secondary metabolism-related genes; thus, this species has the ability to adapt to different light conditions.

Effects of shade stress on turfgrasses morphophysiology and rhizosphere soil bacterial communities.

BACKGROUND: The shade represents one of the major environmental limitations for turfgrass growth. Shade influences plant growth and alters plant metabolism, yet little is known about how shade affects the structure of rhizosphere soil microbial communities and the role of soil microorganisms in plant shade responses. In this study, a glasshouse experiment was conducted to examine the impact of shade on the growth and photosynthetic capacity of two contrasting shade-tolerant turfgrasses, shade-tolerant dwarf lilyturf (Ophiopogon japonicus, OJ) and shade-intolerant perennial turf-type ryegrass (Lolium perenne, LP). We also examined soil-plant feedback effects on shade tolerance in the two turfgrass genotypes. The composition of the soil bacterial community was assayed using high-throughput sequencing. RESULTS: OJ maintained higher photosynthetic capacity and root growth than LP under shade stress, thus OJ was found to be more shade-tolerant than LP. Shade-intolerant LP responded better to both shade and soil microbes than shade-tolerant OJ. The shade and live soil decreased LP growth, but increased biomass allocation to shoots in the live soil. The plant shade response index of LP is higher in live soil than sterile soil, driven by weakened soil-plant feedback under shade stress. In contrast, there was no difference in these values for OJ under similar shade and soil treatments. Shade stress had little impact on the diversity of the OJ and the LP bacterial communities, but instead impacted their composition. The OJ soil bacterial communities were mostly composed of Proteobacteria and Acidobacteria. Further pairwise fitting analysis showed that a positive correlation of shade-tolerance in two turfgrasses and their bacterial community compositions. Several soil properties (NO3--N, NH4+-N, AK) showed a tight coupling with several major bacterial communities under shade stress. Moreover, OJ shared core bacterial taxa known to promote plant growth and confer tolerance to shade stress, which suggests common principles underpinning OJ-microbe interactions. CONCLUSION: Soil microorganisms mediate plant responses to shade stress via plant-soil feedback and shade-induced change in the rhizosphere soil bacterial community structure for OJ and LP plants. These findings emphasize the importance of understanding plant-soil interactions and their role in the mechanisms underlying shade tolerance in shade-tolerant turfgrasses.

Effects of clonal integration on the proximal and distal ramets of Cynodon dactylon under shade stress

ABSTRACT In a pot experiment, clonal ramets of Cynodon dactylon, a stolon herbaceous plant, were treated with heterogeneous lighting. Proximal ramets (elder ramets) were subjected to shade stress at three different degrees, and stolons between proximal and distal ramets of each pair were treated in a connected or severed manner. Results showed that in moderate shade stress, the number of ramets and leaves, biomass, net photosynthetic rate (Pn), maximum quantum yield (Fv/Fm), effective quantum yield of PSII (ФPSII), and soil and plant analysis development values (SPAD) of proximal ramets were significantly reduced, regardless of whether stolons were kept intact or severed. However, the growth of distal ramets was not significantly influenced, and keeping the stolons intact also did not bring apparent benefits for the whole clonal fragments. These results show that clonal integration does not help alleviate the shade stress suffered by proximal ramets and the costs of distal ramets and does not significantly influence the whole clonal fragments. The possible reasons are that distal ramets may be at the cost of metabolism for resource transportation when the proximal ramets suffer from shade stress; thus, clonal integration is not favorable.

The trehalose pathway in maize: conservation and gene regulation in response to the diurnal cycle and extended darkness.

Energy resources in plants are managed in continuously changing environments, such as changes occurring during the day/night cycle. Shading is an environmental disruption that decreases photosynthesis, compromises energy status, and impacts on crop productivity. The trehalose pathway plays a central but not well-defined role in maintaining energy balance. Here, we characterized the maize trehalose pathway genes and deciphered the impacts of the diurnal cycle and disruption of the day/night cycle on trehalose pathway gene expression and sugar metabolism. The maize genome encodes 14 trehalose-6-phosphate synthase (TPS) genes, 11 trehalose-6-phosphate phosphatase (TPP) genes, and one trehalase gene. Transcript abundance of most of these genes was impacted by the day/night cycle and extended dark stress, as were sucrose, hexose sugars, starch, and trehalose-6-phosphate (T6P) levels. After extended darkness, T6P levels inversely followed class II TPS and sucrose non-fermenting-related protein kinase 1 (SnRK1) target gene expression. Most significantly, T6P no longer tracked sucrose levels after extended darkness. These results showed: (i) conservation of the trehalose pathway in maize; (ii) that sucrose, hexose, starch, T6P, and TPS/TPP transcripts respond to the diurnal cycle; and(iii) that extended darkness disrupts the correlation between T6P and sucrose/hexose pools and affects SnRK1 target gene expression. A model for the role of the trehalose pathway in sensing of sucrose and energy status in maize seedlings is proposed.