Physiological stress and higher reproductive success in bumblebees are both associated with intensive agriculture.

Free-living organisms face multiple stressors in their habitats, and habitat quality often affects development and life history traits. Increasing pressures of agricultural intensification have been shown to influence diversity and abundance of insect pollinators, and it may affect their elemental composition as well. We compared reproductive success, body concentration of carbon (C) and nitrogen (N), and C/N ratio, each considered as indicators of stress, in the buff-tailed bumblebee (Bombus terrestris). Bumblebee hives were placed in oilseed rape fields and semi-natural old apple orchards. Flowering season in oilseed rape fields was longer than that in apple orchards. Reproductive output was significantly higher in oilseed rape fields than in apple orchards, while the C/N ratio of queens and workers, an indicator of physiological stress, was lower in apple orchards, where bumblebees had significantly higher body N concentration. We concluded that a more productive habitat, oilseed rape fields, offers bumblebees more opportunities to increase their fitness than a more natural habitat, old apple orchards, which was achieved at the expense of physiological stress, evidenced as a significantly higher C/N ratio observed in bumblebees inhabiting oilseed rape fields.

Ecological Stoichiometry of Bumblebee Castes, Sexes, and Age Groups.

Ecological stoichiometry is important for revealing how the composition of chemical elements of organisms is influenced by their physiological functions and ecology. In this study, we investigated the elemental body composition of queens, workers, and males of the bumblebee Bombus terrestris, an important pollinator throughout Eurasia, North America, and northern Africa. Our results showed that body elemental content differs among B. terrestris castes. Young queens and workers had higher body nitrogen concentration than ovipositing queens and males, while castes did not differ significantly in their body carbon concentration. Furthermore, the carbon-to-nitrogen ratio was higher in ovipositing queens and males. We suggest that high body nitrogen concentration and low carbon-to-nitrogen ratio in young queens and workers may be related to their greater amount of flight muscles and flight activities than to their lower stress levels. To disentangle possible effects of stress in the agricultural landscape, further studies are needed to compare the elemental content of bumblebee bodies between natural habitats and areas of high-intensity agriculture.