Asunto(s)
Fósiles , Imagenología Tridimensional , Difusión de la Información/legislación & jurisprudencia , Publicación de Acceso Abierto/legislación & jurisprudencia , Paleontología/métodos , Paleontología/organización & administración , Animales , Carnivoría , Guías como Asunto , Imagenología Tridimensional/economía , Internet , Bibliotecas Digitales/economía , Bibliotecas Digitales/legislación & jurisprudencia , Museos/economía , Publicación de Acceso Abierto/economía , Paleontología/economía , Paleontología/legislación & jurisprudencia , Cráneo/anatomía & histologíaAsunto(s)
Paleontología/organización & administración , Animales , Evolución Biológica , Humanos , New Mexico , VertebradosAsunto(s)
Arqueología , Paleontología , Distribución por Sexo , Arqueología/organización & administración , Arqueología/estadística & datos numéricos , Docentes/estadística & datos numéricos , Femenino , Humanos , Masculino , Paleontología/organización & administración , Paleontología/estadística & datos numéricos , Investigación/organización & administración , Investigación/estadística & datos numéricos , España , Universidades/organización & administración , Universidades/estadística & datos numéricosRESUMEN
With the introduction of next generation high throughput sequencing in 2005 and the resulting revolution in genetics, ancient DNA research has rapidly developed from an interesting but marginal field within evolutionary biology into one that can contribute significantly to our understanding of evolution in general and the development of our own species in particular. While the amount of sequence data available from ancient human, other animal and plant remains has increased dramatically over the past five years, some key limitations of ancient DNA research remain. Most notably, reduction of contamination and the authentication of results are of utmost importance. A number of studies have addressed different aspects of sampling, DNA extraction and DNA manipulation in order to establish protocols that most efficiently generate reproducible and authentic results. As increasing numbers of researchers from different backgrounds become interested in using ancient DNA technology to address key questions, the need for practical guidelines on how to construct and use an ancient DNA facility arises. The aim of this article is therefore to provide practical tips for building a state-of-the-art ancient DNA facility. It is intended to help researchers new to the field of ancient DNA research generally, and those considering the application of next generation sequencing, in their planning process.
Asunto(s)
ADN/genética , Paleontología/métodos , Animales , Evolución Biológica , ADN/química , Humanos , Laboratorios/organización & administración , Paleontología/organización & administración , Plantas/química , Plantas/genética , Reacción en Cadena de la Polimerasa/métodos , Análisis de Secuencia de ADN/métodos , Manejo de EspecímenesRESUMEN
Even as the division between professional scientists and laypeople became sharper by the end of the nineteenth century, the collaboration of local people remained important in scientific fieldwork, especially in sciences such as vertebrate paleontology that required long-term extractive access to research sites. In the North American West, the competition between museums and universities for the best fossil quarry sites involved negotiations with locals. The conflict over differing conceptions of the field site is vividly demonstrated through an examination of one site on the High Plains of western Nebraska in the early twentieth century. This case offers a rare opportunity to see not only how professionals regarded such sites but also how the resident ranching family, the Cooks, attempted to exercise leverage over the scientific fieldwork that took place there. While the Carnegie Museum of Pittsburgh became mired in protracted conflict with the Cooks over discovery claims and the ongoing control of the site, the University of Nebraska and the American Museum of New York developed more harmonious relations with the site's resident ranching family.