RESUMEN
An effective symbiosis between legumes and rhizobia relies largely on diverse proteins at the plant-rhizobium interface for material transportation and signal transduction during symbiotic nitrogen fixation. Here, we report a comprehensive proteome atlas of the soybean symbiosome membrane (SM), peribacteroid space (PBS), and root microsomal fraction (RMF) using state-of-the-art label-free quantitative proteomic technology. In total, 1759 soybean proteins with diverse functions are detected in the SM, and 1476 soybean proteins and 369 rhizobial proteins are detected in the PBS. The diversity of SM proteins detected suggests multiple origins of the SM. Quantitative comparative analysis highlights amino acid metabolism and nutrient uptake in the SM, indicative of the key pathways in nitrogen assimilation. The detection of soybean secretory proteins in the PBS and receptor-like kinases in the SM provides evidence for the likely extracellular property of the symbiosome and the potential signaling communication between both symbionts at the symbiotic interface. Our proteomic data provide clues for how some of the sophisticated regulation between soybean and rhizobium at the symbiotic interface is achieved, and suggest approaches for symbiosis engineering.
Asunto(s)
Glycine max , Rhizobium , Glycine max/genética , Simbiosis/fisiología , Proteínas de Soja/metabolismo , Proteómica , Fijación del Nitrógeno/fisiología , Rhizobium/química , Rhizobium/metabolismoRESUMEN
Lipopolysaccharides synthesized by rhizobia have a various structure. Differences are observed in lipid A (considered as the most conservative part of LPS), in the core region, and in the O-specific polysaccharide. Lipids A may have different compositions of the sugar backbone and the acylation pattern. The core region of rhizobia mainly consists of hexoses, uronic acids, N-acetylquinohozamine, and Kdo, but has no heptose region typical for enterobacteria. The O-PSs may have a different structure even among strains of the same species. They are built of various monosaccharides and are often hydrophobic. An appropriate structure of LPS domains is required for establishment of an effective symbiosis between bacteria and their plant host. Changes in the structure of LPS (most often caused by mutations) resulted in a decrease in efficiency or failure of atmospheric nitrogen fixation. Complete LPS protects symbiotic bacteria penetrating plant cells and determines the proper organization and maturation of symbiosomes.
Asunto(s)
Lipopolisacáridos/química , Lipopolisacáridos/metabolismo , Plantas/metabolismo , Plantas/microbiología , Rhizobium/química , Rhizobium/metabolismo , Simbiosis , Fijación del NitrógenoRESUMEN
Carbaryl is a widely used carbamate pesticide in agriculture. The strain Rhizobium sp. X9 possesses the typical carbaryl degradation pathway in which carbaryl is mineralized via 1-naphthol, salicylate, and gentisate. In this study, we cloned a carbaryl hydrolase gene cehA and a novel two-component 1-naphthol hydroxylase gene cehC1C2. CehA mediates carbaryl hydrolysis to 1-naphthol and CehC1, an FMNH2 or FADH2-dependent monooxygenase belonging to the HpaB superfamily, and hydroxylates 1-naphthol in the presence of reduced nicotinamide-adenine dinucleotide (FMN)/flavin adenine dinucleotide (FAD), and the reductase CehC2. CehC1 has the highest amino acid similarity (58%) with the oxygenase component of a two-component 4-nitrophenol 2-monooxygenase, while CehC2 has the highest amino acid similarity (46%) with its reductase component. CehC1C2 could utilize both FAD and FMN as the cofactor during the hydroxylation, although higher catalytic activity was observed with FAD as the cofactor. The optimal molar ratio of CehC1 to CehC2 was 2:1. The Km and Kcat/Km values of CehC1 for 1-naphthol were 74.71 ± 16.07 µM and (8.29 ± 2.44) × 10-4 s-1·µM-1, respectively. Moreover, the enzyme activities and substrate spectrum between CehC1C2 and previously reported 1-naphthol hydroxylase McbC were compared. The results suggested that McbC had a higher 1-naphthol hydroxylation activity, while CehC1C2 had a broader substrate spectrum.
Asunto(s)
Proteínas Bacterianas/metabolismo , Carbaril/metabolismo , Hidrolasas/metabolismo , Oxigenasas de Función Mixta/metabolismo , Rhizobium/metabolismo , Proteínas Bacterianas/química , Proteínas Bacterianas/genética , Biodegradación Ambiental , Coenzimas/metabolismo , Mononucleótido de Flavina/metabolismo , Flavina-Adenina Dinucleótido/metabolismo , Hidrolasas/química , Hidrolasas/genética , Cinética , Oxigenasas de Función Mixta/química , Oxigenasas de Función Mixta/genética , Naftoles/metabolismo , Plaguicidas/química , Plaguicidas/metabolismo , Rhizobium/química , Rhizobium/enzimología , Rhizobium/genéticaRESUMEN
Four strains, coded as UPM1132, UPM1133T, UPM1134 and UPM1135, and isolated from nodules of Pisum sativum plants grown on Ni-rich soils were characterised through a polyphasic taxonomy approach. Their 16S rRNA gene sequences were identical and showed 100% similarity with their closest phylogenetic neighbors, the species included in the 'R. leguminosarum group': R. laguerreae FB206T, R. leguminosarum USDA 2370T, R. anhuiense CCBAU 23252T, R. sophoreae CCBAU 03386T, R. acidisoli FH13T and R. hidalgonense FH14T, and 99.6% sequence similarity with R. esperanzae CNPSo 668T. The analysis of combined housekeeping genes recA, atpD and glnII sequences showed similarities of 92-95% with the closest relatives. Whole genome average nucleotide identity (ANI) values were 97.5-99.7% ANIb similarity among the four strains, and less than 92.4% with closely related species, while digital DNA-DNA hybridization average values (dDDH) were 82-85% within our strains and 34-52% with closely related species. Major fatty acids in strain UPM1133T were C18:1 ω7c / C18:1 ω6c in summed feature 8, C14:0 3OH/ C16:1 iso I in summed feature 2 and C18:0. Colonies were small to medium, pearl-white coloured in YMA at 28°C and growth was observed in the ranges 8-34°C, pH 5.5-7.5 and 0-0.7% (w/v) NaCl. The DNA G+C content was 60.8mol %. The combined genotypic, phenotypic and chemotaxonomic data support the classification of strains UPM1132, UPM1133T, UPM1134 and UPM1135 into a novel species of Rhizobium, for which the name Rhizobium ruizarguesonis sp. nov. is proposed. The type strain is UPM1133T (=CECT 9542T=LMG 30526T).
Asunto(s)
Pisum sativum/microbiología , Rhizobium/clasificación , Rhizobium/fisiología , Nódulos de las Raíces de las Plantas/microbiología , ADN Bacteriano/genética , Ácidos Grasos/análisis , Genes Bacterianos/genética , Genoma Bacteriano/genética , Genotipo , Hibridación de Ácido Nucleico , Fenotipo , Filogenia , ARN Ribosómico 16S/genética , Rhizobium/química , Rhizobium/citología , Análisis de Secuencia de ADN , Suelo/química , Microbiología del Suelo , SimbiosisRESUMEN
Fluorescent nitrogen-doped carbon dots (CDs) were prepared via hydrothermal method at 190 °C for 10 h using rhizobium from soy as the carbon and nitrogen source. Their optical properties, structure, morphology, and functional groups were characterized in detail and the results showed that they possess unique excitation-dependent fluorescence behavior, with average diameter 4.5 ± 2.0 nm and good water dispersibility. Due to the overlap of the UV-vis absorbance of chlortetracycline hydrochloride (CCH) and the fluorescence excitation band of CDs, the fluorescence of the prepared CDs can be quenched by CCH selectively and sensitively. The changes of the fluorescence intensity of CDs have a good linear relationship with the concentration of CCH in a wide concentration range of 5-100 µM, with a detection limit of 0.254 µM. This present method has been successfully applied to determine the CCH in water with recovery ranging from 96.0% to 100.7%.
Asunto(s)
Carbono/química , Clortetraciclina/análisis , Colorantes Fluorescentes/química , Nitrógeno/química , Puntos Cuánticos/química , Contaminantes Químicos del Agua/análisis , Rhizobium/química , Espectrometría de FluorescenciaRESUMEN
A Gram-stain-negative, facultative aerobic, non-spore-forming, non-motile, non-flagellated, rod-shaped bacterium, designated strain NAU-18T was isolated from an oil-contaminated soil in China. Strain NAU-18T could grow at 10-42 °C (optimum, 30 °C), at pH 5.0-8.0 (optimum, 7.0) and in the presence of 0-2.0% (w/v) NaCl (optimum, 0.5% NaCl in R2A). The predominant fatty acids were C18:1ω7c (71.2%) and Summed feature 2 (5.1%), representing 76.3% of the total fatty acids. The major respiratory quinones were Q9 and Q10. The DNA G + C content of strain NAU-18T was 61.4 mol% based on its draft genome sequence. Genome annotation of strain NAU-18T predicted the presence of 6668 genes, of which 6588 are coding proteins and 80 are RNA genes. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain NAU-18T was a member of the genus Rhizobium and showed 96.93% (with 93.2% coverage) and 96.81% (with 100% coverage) identities with those of Neorhizobium alkalisoli CCBAU 01393T and Rhizobium oryzicola ZYY136T, respectively. In the phylogenetic analysis, strain NAU-18T and R. oryzicola ZYY136T are consistently placed in the same branch. Strain NAU-18T represents a novel species within the genus Rhizobium, for which the name Rhizobium terrae sp. nov. is proposed, with the type strain NAU-18T (=KCTC 62418T = CCTCC AB 2018075T).
Asunto(s)
Yacimiento de Petróleo y Gas/microbiología , Rhizobium/clasificación , Microbiología del Suelo , Composición de Base , China , ADN Bacteriano/genética , Ácidos Grasos/química , Genes Bacterianos , Genoma Bacteriano/genética , Concentración de Iones de Hidrógeno , Hibridación de Ácido Nucleico , Filogenia , Quinonas/química , ARN Ribosómico 16S/genética , Rhizobium/química , Rhizobium/citología , Rhizobium/fisiología , Análisis de Secuencia de ADN , Cloruro de Sodio/metabolismo , Especificidad de la Especie , TemperaturaRESUMEN
Endophytic bacteria, as the most promising components of effective, biofertilizers biostimulating and biocontrol preparations, should be very intensively obtained from various plants and studied in terms of the conditions determining the potential ability to promote plant growth. For this reason, endophytic bacteria have been isolated from both stems and roots of up to six systematically distant species of vascular plants: one species belonging to the seedless vascular plants (Monilophyta), and five seed plants (Spermatophyta). The 23 isolated strains represented nine genera: Delftia, Stenotrophomonas, Rhizobium, Brevundimonas, Variovorax, Achromobacter, Novosphingobium, Comamonas and Collimonas, notably which were closely related-belonging to the phylum Proteobacteria. Stenotrophomonas sp. strains showed the greatest ability to synthesize indole-3-acetic acid (IAA)-like compounds, while Achromobacter sp. strains produced the highest levels of siderophores. The presence of the nifH gene and nitrogen binding activity was demonstrated for 95% of the strains tested. Stenotrophomonas maltophila (ES2 strain) showed the highest metabolic activity based on Biolog GEN III test. The ability to solubilize phosphate was determined only for three tested strains from genus: Delftia, Rhizobium and Novosphingobium. The presented work demonstrated that the metabolic and phenotypic properties of plant growth-promoting endophytes are correlated with the genus of bacteria and are not correlated with the host plant species or part of plant (stem, root).
Asunto(s)
Bacterias/metabolismo , Plantas/microbiología , Bacterias/aislamiento & purificación , Ácidos Indolacéticos/química , Ácidos Indolacéticos/metabolismo , Ácidos Indolacéticos/farmacología , Fenotipo , Desarrollo de la Planta/efectos de los fármacos , Raíces de Plantas/microbiología , Análisis de Componente Principal , Rhizobium/química , Rhizobium/aislamiento & purificación , Rhizobium/metabolismo , Sideróforos/metabolismo , Sideróforos/farmacología , Stenotrophomonas/química , Stenotrophomonas/aislamiento & purificación , Stenotrophomonas/metabolismo , Vicia faba/crecimiento & desarrollo , Vicia faba/microbiología , Zea mays/crecimiento & desarrollo , Zea mays/microbiologíaRESUMEN
Phaseolus vulgaris is a legume indigenous to America which is currently cultivated in Europe including countries located at the Southeast of this continent, such as Croatia, where several local landraces are cultivated, most of them of Andean origin. In this work we identify at species and symbiovar levels several fast-growing strains able to form effective symbiosis with P. vulgaris in different Croatian soils. The identification at species level based on MALDI-TOF MS and core gene sequence analysis showed that most of these strains belong to the species R. leguminosarum, R. hidalgonense and R. pisi. In addition, several strains belong to putative new species phylogenetically close to R. ecuadorense and R. sophoriradicis. All Croatian strains belong to the symbiovar phaseoli and harbour the α and γ nodC alleles typical for American strains of this symbiovar. Nevertheless, most of Croatian strains harboured the γ nodC gene allele supporting its Andean origin since it is also dominant in other European countries, where Andean cultivars of P. vulgaris are traditionally cultivated, as occurs in Spain. The only strains harbouring the α nodC allele belong to R. hidalgonense and R. pisi, this last only containing the symbiovars viciae and trifolii to date. This is the first report about the presence in Europe of the species R. hidalgonense, the nodulation of P. vulgaris by R. pisi and the existence of the symbiovar phaseoli within this species. These results significantly increase the knowledge of the biogeography of Rhizobium-P. vulgaris symbiosis.
Asunto(s)
Biodiversidad , Phaseolus/microbiología , Filogenia , Rhizobium/clasificación , Rhizobium/genética , Nódulos de las Raíces de las Plantas/microbiología , Proteínas Bacterianas/genética , Croacia , ADN Bacteriano/genética , Técnica del ADN Polimorfo Amplificado Aleatorio , Rhizobium/química , Análisis de Secuencia de ADN , Microbiología del Suelo , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción , Simbiosis/genéticaRESUMEN
Acid soils constitute a severe problem for leguminous crops mainly through a disturbance in rhizobium-legume interactions. Rhizobium favelukesii-an acid-tolerant rhizobium able to nodulate alfalfa-is highly competitive for nodule occupation under acid conditions but inefficient for biologic nitrogen fixation. In this work, we obtained a general description of the acid-stress response of R. favelukesii LPU83 by means of proteomics by comparing the total proteome profiles in the presence or absence of acid stress by nanoflow ultrahigh-performance liquid chromatography coupled to mass spectrometry. Thus, a total of 336 proteins were identified with a significant differential expression, 136 of which species were significantly overexpressed and 200 underexpressed in acidity. An in silico functional characterization with those respective proteins revealed a complex and pleiotropic response by these rhizobia involving components of oxidative phosphorylation, glutamate metabolism, and peptidoglycan biosynthesis, among other pathways. Furthermore, a lower permeability was evidenced in the acid-stressed cells along with several overexpressed proteins related to γ-aminobutyric acid metabolism, such as the gene product of livK, which gene was mutated. This mutant exhibited an acid-sensitive phenotype in agreement with the proteomics results. We conclude that both the γ-aminobutyric acid metabolism and a modified cellular envelope could be relevant to acid tolerance in R. favelukesii.
Asunto(s)
Proteínas Bacterianas/análisis , Proteómica/métodos , Rhizobium/química , Estrés Fisiológico/efectos de los fármacos , Ácidos/farmacología , Proteínas Bacterianas/fisiología , Permeabilidad de la Membrana Celular , Cromatografía Líquida de Alta Presión , Espectrometría de Masas , Mutación , Nodulación de la Raíz de la Planta , Rhizobium/fisiología , Suelo/química , Ácido gamma-Aminobutírico/genética , Ácido gamma-Aminobutírico/metabolismoRESUMEN
The endogenous esters of orthophthalic acid, dibutyl phthalate (DBP) and di-(2-ethylhexyl) phthalate (DEHP), have been first detected in bacterial pathogens of plants (Clavibacter michiganensis ssp. sepedonicus, Pectobacterium carotovorum ssp. carotovorum, Rhizobium rhizogenes, Rhizobium radiobacter) and bacterial pathogens of animal (Escherichia coli).
Asunto(s)
Dibutil Ftalato/análisis , Dietilhexil Ftalato/análisis , Escherichia coli/química , Pectobacterium/química , Rhizobium/químicaRESUMEN
Phaseolus dumosus is an endemic species from mountain tops in Mexico that was found in traditional agriculture areas in Veracruz, Mexico. P. dumosus plants were identified by ITS sequences and their nodules were collected from agricultural fields or from trap plant experiments in the laboratory. Bacteria from P. dumosus nodules were identified as belonging to the phaseoli-etli-leguminosarum (PEL) or to the tropici group by 16S rRNA gene sequences. We obtained complete closed genomes from two P. dumosus isolates CCGE531 and CCGE532 that were phylogenetically placed within the tropici group but with a distinctive phylogenomic position and low average nucleotide identity (ANI). CCGE531 and CCGE532 had common phenotypic characteristics with tropici type B rhizobial symbionts. Genome synteny analysis and ANI showed that P. dumosus isolates had different chromids and our analysis suggests that chromids have independently evolved in different lineages of the Rhizobium genus. Finally, we considered that P. dumosus and Phaseolus vulgaris plants belong to the same cross-inoculation group since they have conserved symbiotic affinites for rhizobia.
Asunto(s)
Phaseolus/microbiología , Filogenia , Rhizobium/clasificación , Rhizobium/genética , Nódulos de las Raíces de las Plantas/microbiología , Simbiosis , Evolución Biológica , ADN Bacteriano/genética , Genes Bacterianos/genética , Variación Genética , Genoma Bacteriano/genética , México , Hibridación de Ácido Nucleico , Phaseolus/clasificación , Plásmidos/genética , ARN Ribosómico 16S/genética , Replicón/genética , Rhizobium/química , Rhizobium/fisiología , Análisis de Secuencia de ADNRESUMEN
AIMS: Because the effect of Cd2+ -immobilized microbe on Cd uptake of plants in Cd-contaminated soil remains underexplored, this study focuses on the effect of Cd2+ -immobilized rhizobia on Cd uptake of soybean. METHODS AND RESULTS: Strain KG2 from soybean nodule was identified as Rhizobium pusense KG2 by phylogenetic analysis. Rhizobium pusense KG2 showed the 120 mg l-1 of minimal lethal concentration for Cd2+ . In 50 and 100 mg l-1 of Cd2+ liquid, approximately 2 × 1010 cells removed 56·71 and 22·11% of Cd2+ , respectively. In pot soil containing 50 and 100 mg kg-1 of Cd2+ , strain KG2 caused a 45·9 and 35·3% decrease in soybean root Cd content, respectively. Meanwhile, KG2 improved the root and shoot length, nitrogen content and biomass of soybean plants and superoxide dismutase activity. CONCLUSIONS: The Cd2+ -immobilized rhizobia could inhibit soybean plants to absorb Cd2+ from soil, promote plant growth and improve plant's tolerance against Cd. This study is the first time to report that R. pusense is an effective nodulating rhizobium of legume. SIGNIFICANCE AND IMPACT OF THE STUDY: Some Cd2+ -immobilized microbe lowering Cd uptake of plant and promoting plant growth should be considered as an effective strategy for producing safety crops in the Cd-contaminated agricultural soil.
Asunto(s)
Cadmio , Células Inmovilizadas , Glycine max , Rhizobium , Agricultura , Cadmio/análisis , Cadmio/química , Cadmio/metabolismo , Células Inmovilizadas/química , Células Inmovilizadas/metabolismo , Contaminación de Alimentos/prevención & control , Raíces de Plantas/química , Raíces de Plantas/metabolismo , Rhizobium/química , Rhizobium/metabolismo , Contaminantes del Suelo/análisis , Contaminantes del Suelo/química , Contaminantes del Suelo/metabolismo , Glycine max/química , Glycine max/metabolismoRESUMEN
The presence of harmful contaminants in the waste stream is an important concern worldwide. The convergence of biotechnology and nanoscience offers a sustainable alternative in treating contaminated waters. Hexavalent chromium, being carcinogenic deserves effective and sustainable methods for sequestration. Here in, we report the immobilization of a prokaryote (Rhizobium) and eukaryote (Saccharomyces cerevisiae) in multiwalled carbon nanotubes (MWCNTs) for the effective adsorption of hexavalent chromium. The carboxylic groups were introduced into the MWCNTs during oxidation using potassium permanganate and were subjected to EDC-HOBT coupling to bind with microbial cell surface. FTIR, TGA, BET, FESEM-EDAX, HRTEM, XPS and confocal microscopy were the investigative techniques used to characterize the developed biosorbents. Experimental variables such as pH, adsorbent dosage, kinetics, isotherms and thermodynamics were investigated and it was observed that the system follows pseudo second order kinetics with a best fit for Langmuir isotherm. Electrostatic interactions between the functional groups in the microbial cell wall and hydrochromate anion at pH 2.0 propel the adsorption mechanism. The lab scale column studies were performed with higher volumes of the Cr(VI) contaminated water. Sodium hydroxide was used as the desorbing agent for reuse of the biosorbents. The sustainable biosorbents show prospects to treat chromium contaminated water.
Asunto(s)
Cromo/análisis , Cromo/aislamiento & purificación , Nanotubos de Carbono/química , Rhizobium/química , Saccharomyces cerevisiae/química , Contaminantes Químicos del Agua/análisis , Contaminantes Químicos del Agua/aislamiento & purificación , Purificación del Agua/métodos , Cromo/química , Rhizobium/metabolismo , Saccharomyces cerevisiae/metabolismo , Contaminantes Químicos del Agua/químicaRESUMEN
Agrocin 108 is a 3'-O-ß-D-xylopyranosyl-cytidine-5'-O-phosphodiester of an ascorbate-carbocyclic cyclopentenone analogue, with bacteriocin-like properties. This bacteriocin exhibits orders of magnitude greater than the inhibition zone diameter towards the indicator strain than either ampicillin or streptomycin. It has been isolated from cultures of Rhizobium rhizogenes strain K108. The structure of the agrocin 108 without detail, has been previously published. We now report a detailed structure elucidation, including the hitherto undetermined residual 5'-phospho-diester fragment by a combination of 1D and 2D NMR studies at various pH values in H2O/D2O, high resolution MS, pKa determination, and chemical degradation.
Asunto(s)
Bacteriocinas/química , Bacteriocinas/farmacología , Bacterias/efectos de los fármacos , Citidina/análisis , Electroforesis en Papel , Formaldehído/análisis , Espectroscopía de Resonancia Magnética , Espectrometría de Masas , Pruebas de Sensibilidad Microbiana , Conformación Molecular , Rhizobium/química , Rhizobium/efectos de los fármacos , Rhizobium/metabolismo , Xilosa/análisisRESUMEN
The pentasaccharide fragment α-d-Man-(1 â 5)-[α-d-Kdo-(2 â 4)-]α-d-Kdo-(2 â 6)-ß-d-GlcNAc-(1 â 6)-α-d-GlcNAc equipped with a 3-aminopropyl spacer moiety was prepared by a sequential assembly of monosaccharide building blocks. The glucosamine disaccharide-as a backbone surrogate of the bacterial lipid A region-was synthesized using an 1,3-oxazoline donor, which was followed by coupling with an isopropylidene-protected Kdo-fluoride donor to afford a protected tetrasaccharide intermediate. Eventually, an orthogonally protected manno-configured trichloroacetimidate donor was used to achieve the sterically demanding glycosylation of the 5-OH group of Kdo in good yield. The resulting pentasaccharide is suitably protected for further chain elongation at positions 3, 4, and 6 of the terminal mannose. Global deprotection afforded the target pentasaccharide to be used for the conversion into neoglycoconjugates and "clickable" ligands.
Asunto(s)
Lipopolisacáridos/síntesis química , Oligosacáridos/química , Rhizobium/química , Disacáridos/síntesis química , Lipopolisacáridos/químicaRESUMEN
To maximize the contribution of biological nitrogen fixation in common bean, it is necessary to use bacterial strains that are more adapted, competitive, and efficient in the symbiotic process. In this regard, the aim of this study was to evaluate the agronomic efficiency (AE) of three bacterial strains isolated from acid soils with high Al content from the Amazon region in an Argissolo Vermelho Distrófico típico soil (Typic Rhodustults - USDA Classification) from the municipality of Formiga, MG, Brazil. We compared their AE to that of the reference strain CIAT 899T and of two controls without inoculation (one without and another with 80 kg ha-1 of N-urea). The results indicated that inoculation with the strains UFLA 02-100 and UFLA 02-127 provides grain yield equivalent to inoculation with the reference strain and to the control with mineral N. Thus, both have potential for recommendation as inoculants for common bean.
Para maximizar a contribuição da fixação biológica de nitrogênio no feijoeiro-comum é necessária a utilização de estirpes de bactérias mais adaptadas, competitivas e eficientes no processo simbiótico. Nesse sentido, objetivou-se avaliar, em um Argissolo Vermelho Distrófico típico do município de Formiga-MG, a eficiência agronômica (EA) de três estirpes isoladas de solos ácidos e com alto teor de Al da Amazônia e comparar suas EA à da estirpe referência CIAT 899T e à de dois controles sem inoculação (um sem e outro com 80 kg ha-1 of N-ureia). Os resultados indicaram que a inoculação com as estirpes UFLA 02-100 e UFLA 02-127 propicia rendimento de grãos equivalente ao da estirpe referência e ao do controle com N mineral e que por isso, ambas têm potencial para ser recomendadas como inoculantes para o feijoeiro-comum.
Asunto(s)
Acidez del Suelo , Fijación del Nitrógeno , Inoculantes Agrícolas , Phaseolus nanus/análisis , Rhizobium/químicaRESUMEN
Symbiotic rhizobia in legumes account for a large portion of nitrogen fixation in the biosphere. Nitrogen fixation is an energy-demanding process requiring tight control of metabolism and redox state. It is of great interest to understand the bacteroid differentiation process and the roles of energy storage molecules, such as glycogen and polyhydroxybutyrate (PHB), in maintaining the Rhizobium-legume symbioses. Traditional biochemical assays for checking phenotypic changes of mutants require a large volume of starting materials, which is difficult for unculturable, terminally differentiated bacteroids. Here we present a label-free technique that allows the identification and characterization of phenotypic changes of bacteria at the single-cell level. This work demonstrates the application of single-cell Raman spectra (SCRS) to differentiate Rhizobium leguminosarum bv. viciae wild-type and mutants under different conditions. We found symbiotically differentiated bacteroids and free-living bacteria differed primarily at a Raman biomarker, cytochrome c, corresponding to a bacteroid-specific terminal oxidase. We demonstrated that, for the first time, SCRS were able to link phenotypic changes and specific genetic mutants, in this case, single and double mutations in synthesis of carbon storage molecules glycogen and polyhydroxybutyrate (PHB). By analyzing SCRS of these mutants, it provides insights into metabolite production and carbon regulatory network of rhizobia.
Asunto(s)
Proteínas Bacterianas/genética , Rhizobium/química , Rhizobium/citología , Análisis de la Célula Individual , Análisis Multivariante , Mutación , Fenotipo , Espectrometría RamanRESUMEN
Eight mesorhizobial symbiotic strains isolated from Anthyllis vulneraria root-nodules were studied and compared taxonomically with defined Mesorhizobium species. All strains presented identical 16S rDNA sequences but can be differentiated by multilocus sequence analysis of housekeeping genes (recA, atpD, glnII and dnaK). Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry analyses separate these strains in two groups and a separate strain. Levels of DNA-DNA relatedness were less than 55% between representative strains and their closest Mesorhizobium reference relatives. The two groups containing four and three strains, respectively, originating from border mine and non-mining areas in Cévennes, were further phenotypically characterized. Groupings were further supported by average nucleotide identity values based on genome sequencing, which ranged from 80 to 92% with their close relatives and with each other, confirming these groups represent new Mesorhizobium species. Therefore, two novel species Mesorhizobium delmotii sp. nov. (type strain STM4623T=LMG 29640T=CFBP 8436T) and Mesorhizobium prunaredense sp. nov. (type strain STM4891T=LMG 29641T=CFBP 8437T) are proposed. Type strains of the two proposed species share accessory common nodulation genes within the new symbiovar anthyllidis as found in the Mesorhizobium metallidurans type strain.
Asunto(s)
Fabaceae/microbiología , Mesorhizobium/clasificación , Rhizobium/clasificación , Nódulos de las Raíces de las Plantas/microbiología , Simbiosis , Composición de Base , Genoma Bacteriano , Espectrometría de Masas , Mesorhizobium/química , Mesorhizobium/genética , Tipificación de Secuencias Multilocus , Filogenia , ARN Ribosómico 16S/genética , Rhizobium/química , Rhizobium/genética , Análisis de Secuencia de ADNRESUMEN
The upstream sequences of gene coding sequences are termed as promoter sequences. Studying the expression patterns of promoters are very significant in understanding the gene regulation and spatiotemporal expression patterns of target genes. On the other hand, it is also critical to establish promoter evaluation tools and genetic transformation techniques that are fast, efficient, and reproducible. In this study, we investigated the spatiotemporal expression pattern of the rhizobial symbiosis-specific nodule inception (NIN) promoter of Phaseolus vulgaris in the transgenic hairy roots. Using plant genome databases and analysis tools we identified, isolated, and cloned the P. vulgaris NIN promoter in a transcriptional fusion to the chimeric reporter ß-glucuronidase (GUS) GUS-enhanced::GFP. Further, this protocol describes a rapid and versatile system of genetic transformation in the P. vulgaris using Agrobacterium rhizogenes induced hairy roots. This system generates ≥2 cm hairy roots at 10 to 12 days after transformation. Next, we assessed the spatiotemporal expression of NIN promoter in Rhizobium inoculated hairy roots at periodic intervals of post-inoculation. Our results depicted by GUS activity show that the NIN promoter was active during the process of nodulation. Together, the present protocol demonstrates how to identify, isolate, clone, and characterize a plant promoter in the common bean hairy roots. Moreover, this protocol is easy to use in non-specialized laboratories.
Asunto(s)
Nodulación de la Raíz de la Planta/genética , Rhizobium/genética , Regulación de la Expresión Génica de las Plantas , Raíces de Plantas/química , Raíces de Plantas/genética , Regiones Promotoras Genéticas , Rhizobium/químicaRESUMEN
Soil-dwelling, nitrogen-fixing rhizobia signal their presence to legume hosts by secreting lipo-chitooligomers (LCOs) that are decorated with a variety of chemical substituents. It has long been assumed, but never empirically shown, that the LCO backbone is synthesized first by NodC, NodB, and NodA, followed by addition of one or more substituents by other Nod proteins. By analyzing a collection of in-frame deletion mutants of key nod genes in the bacterium Rhizobium sp. IRBG74 by mass spectrometry, we were able to shed light on the possible substitution order of LCO decorations, and we discovered that the prevailing view is probably erroneous. We found that most substituents could be transferred to a short chitin backbone prior to acylation by NodA, which is probably one of the last steps in LCO biosynthesis. The existence of substituted, short chitin oligomers offers new insights into symbiotic plant-microbe signaling.