The effects of ß-mannanase supplementation on growth performance, digestive enzyme activity, cecal microbial communities, and short-chain fatty acid production in broiler chickens fed diets with different metabolizable energy levels.

This study assessed the effects of ß-mannanase (BM) supplementation on growth performance, digestive enzyme activity, cecal microbial communities, and short-chain fatty acid (SCFA) production in broiler chickens fed diets with different metabolizable energy (ME) levels. A total of 1,296 male 1-d-old Cobb 500 broilers were randomly distributed in a 3 × 2 factorial arrangement (3 ME levels × 0 or 200 g/ton BM), with 6 replicates per treatment combination. The 3 ME levels were 3,000 (ME1), 2,930 (ME2), and 2,860 (ME3) kcal/kg, respectively, during the 0 to 3 wk-old stages and 3,150 (ME1), 3,080 (ME2), and 3,010 (ME3) kcal/kg, respectively, during the 3 to 6 wk-old stages. Reducing ME levels increased broiler feed intake (P = 0.036) and decreased average daily gain (ADG, P = 0.002) during the entire period. While BM supplementation increased ADG (P = 0.002) and improved the feed conversion ratio (P = 0.001) during the 0 to 3 wk-old stages, with no effect during the 3 to 6 wk-old stages. Overall, reducing ME levels increased pancreatic lipase (P = 0.045) and amylase (P = 0.013) activity and duodenal amylase activity (P = 0.047). Notably, BM supplementation significantly increased pancreatic lipase activity (P = 0.015) and increased lipase (P = 0.029) and amylase (P = 0.025) activities in the jejunal chyme. Although diet or enzyme supplementation did not affect microbial diversity, significant differences in microbial communities were observed. At the genus level, decreasing ME levels significantly affected the average abundances of Tyzzerella (P = 0.028), Candidatus_Bacilloplasma (P = 0.001), Vibrio (P = 0.005), and Anaerotruncus (P = 0.026) among groups, whereas BM supplementation reduced the average abundances of Escherichia-Shigella (P = 0.048) and increased the average abundances of Barnesiella (P = 0.047), Ruminococcus (P = 0.020), Alistipes (P = 0.050), and Lachnospiraceae_unclassified (P = 0.009). SCFA concentrations strongly depended on bacterial community composition, and BM supplementation increased acetic acid (P = 0.004), propionic acid (P = 0.016), and total SCFA concentrations. In conclusion, BM supplementation improved the performance of younger broilers, and both enzyme supplementation and reduced ME levels positively affected digestive enzyme activity and intestinal microflora.

The broiler industry currently faces numerous challenges, especially the significant rise in feedstuff prices, and the economic considerations with regard to feedstuff formulation have become important factors for reducing costs and enhancing benefits. Low-energy diets have been increasingly suggested to control feed costs; however, lower nutrient concentrations may result in reduced performance, thereby increasing attention toward the potential use of feed enzymes. In this study, we have assessed the impacts of ß-Mannanase (BM), the major ß-mannan degrading enzyme, supplementation on poultry health and digestion. Our study involved a total of 1,296 Cobb broilers, randomly distributed into a 3 × 2 factorial arrangement to evaluate the effects of BM supplementation on growth, digestive enzyme activity, cecal microbial communities, and SCFA production in broiler chickens fed diets with varying ME levels. Our findings confirmed previous research by showing that BM-supplemented diets are effective in improving performance in younger broilers. In addition, we found that both BM supplementation and reduced ME levels also have positive impacts on digestive enzyme activity and intestinal flora in chickens, which might account for improving production performance. These results will be a crucial refence for the scientific implementation of BM supplementation, which may help achieve better performance and intestinal microecology, resulting in higher economic productivity of broiler farms.

Inclusion of dietary ß-mannanase improves performance and ileal digestibility and reduces ileal digesta viscosity of broilers fed corn-soybean meal based diet.

This study was aimed to evaluate the influence of dietary ß-mannanase inclusion on growth performance, apparent ileal digestibility, digesta viscosity, blood metabolites and excreta noxious gas emissions in broilers fed corn-soybean meal based diet. A total of 600 conventional healthy 1-d-old ROSS 308 broilers with body weight 45 ± 0.50 g (mean ± SD) were randomly assigned to 4 dietary treatments with 10 replicates cages, with 15 broilers in each and fed basal diet supplemented to corn-SBM based diets with 0, 2400, 4800, and 7200 MNU ß-mannanase/kg for 35 d feeding trial period. Significant results were observed on improved average daily gain and reduced feed conversion ratio during trial period and also reduced ileal digesta viscosity and improved apparent ileal digestibility of dry matter, nitrogen and energy. However, no significant effects were found on blood urea nitrogen and creatinine, excreta noxious gas emissions. In conclusion, the inclusion of dietary ß-mannanase had potential to improve daily gain and feed efficiency and apparent ileal digestibility while decreasing digesta viscosity of broiler.

The effect of ß-mannanase on nutrient utilization and blood parameters in chicks fed diets containing soybean meal and guar gum.

The present study was conducted to determine whether the addition of ß-mannanase in broiler feed changes hormonal profiles in the blood and broiler performance and nutrient availability. Five hundred and four Cobb male chickens were studied during d 7 to 21. Three corn-soybean meal (SBM) based diets 1) Low SBM (18% SBM); 2) High SBM (31% SBM); and 3) High SBM+GG (31% SBM + Guar Gum (GG) 0.5%) with 3 levels of ß-mannanase (0, 200, and 400 ppm) were mixed to produce 9 diets. A factorial design 3 × 3 was performed with JMP pro 13 (SAS, 2017). Analysis of variance and contrast analysis were used to test significance level at P < 0.05. Glucose (190 and 188 mg/dL) was increased with 200 and 400 ppm of ß-mannanase, respectively, compared to control (182 mg/dL) in the fasted state (P < 0.037). Glucose was higher in chicks fed with the High SBM and High SBM + GG diets but lower in the fasted re-fed state (P < 0.01). Insulin was higher with 200 and 400 ppm added ß-mannanase in the fed state (P < 0.021). Insulin-like growth factor-1 was higher with 400 ppm added to High SBM+GG. ß-mannanase improved feed conversion ratio (FCR) 9 points with 400 ppm in High SBM diet (P < 0.01) and 16 and 18 points with 200 and 400 ppm, respectively, added to the High SBM+GG diet (P < 0.01). Viscosity decreased from 19.2 to 7 cps with both enzyme doses in the High SBM + GG diet (P < 0.01). Digestible energy was +152 kcal/kg with 400 ppm ß-mannanase in the High SBM diet and +200 kcal/kg with both levels of enzyme in High SBM+GG diet. Digestibility of amino acids was improved from 0.8 to 3.6% with ß-mannanase in High SBM+GG diet (P < 0.05). In conclusion, chicks fed with High SBM and High SBM+GG diets with added ß-mannanase significantly improved blood glucose and anabolic hormone homeostasis, FCR, digestible energy, and digestible amino acids compared to chicks fed with same diets without ß-mannanase.

Changes in immune and metabolic gut response in broilers fed ß-mannanase in ß-mannan-containing diets.

ß-galactomannans found in soy-based broiler feed are known to cause physiological effects that are hypothesized to be related to gut inflammation. Previous studies have shown that the incorporation of ß-mannanase in the diet or as a supplement results in improvements to certain performance parameters related to gut health and feed conversion. Using kinome analysis, we characterized the mechanism of ß-galactomannan activity and supplementation with ß-mannanase on the gut of commercial broilers to understand the mode of action. Two doses of ß-mannanase (200 and 400 g/ton of feed) with and without inclusion of additional ß-galactomannan (3,000 ppm) were tested at 3 time points (d 14, d 28, and d 42 post hatch). Broilers were fed starter (d 0 to 14), grower (d 15 to 28), and finisher diets (d 29 to 42). Jejuna were collected from birds from each treatment condition and time point. Cluster analysis of the kinome data showed that birds clustered first by age, then predominantly by whether ß-mannanase had been included in the diet. Biological pathway analysis showed that the inclusion of additional ß-galactomannan into the diet resulted in increased signaling related to immune response, relative to our normal control diet (with reduced soybean meal). The addition of ß-mannanase to the enhanced ß-galactomannan diet eliminated the majority of this immune-related signaling, indicating that the feed-induced immune response within the jejuna had been eliminated by the addition of ß-mannanase. We also saw changes in specific metabolic and gut function pathways in birds fed ß-mannanase. These observed changes in ß-mannanase-fed birds are likely the mechanism for the enhanced performance and feed conversion observed in birds given ß-mannanase in their diets.

Effects of mannan level and ß-mannanase supplementation on growth performance, apparent total tract digestibility and blood metabolites of growing pigs.

The exogenous enzymes are less consistent in their effects as their beneficial effects depend upon the types and level non-starch polysaccharides (NSP) present in the diets. Therefore, exogenous enzymes should be selected on the basis of types and amount of the NSP in the pig diets. The objectives of the present experiments were to investigate the effects of dietary level of mannan and ß-mannanase supplementation on growth performance, apparent total tract digestibility (ATTD) of energy and nutrients, and blood metabolites of growing pigs. In Exp. 1, 96 barrows were randomly allotted to four treatments on the basis of BW. There were four replicates in each treatment with six pigs per replicate. The dietary treatments were a corn-soybean meal (SBM)-based control diet and three other diets consisted of the control diet supplemented with 400, 800 or 1600 U of ß-mannanase/kg diet. The final BW, average daily gain (ADG) and blood glucose increased (linear, P0.05) on the concentrations of blood total cholesterol, triacylglycerides and blood urea nitrogen. These results indicate that supplementation of ß-mannanase to low- or high-mannan diets have potential to improve the performance of growing pigs. In addition, palm kernel meal may partially replace corn and SBM without reducing pig performance if ß-mannanase is added to diet.

Evaluation of inclusion level of wheat distillers dried grains with solubles with and without protease or ß-mannanase on performance and water intake of turkey hens.

It is becoming a common practice to use higher levels of wheat distillers dried grains with solubles (wDDGS) in poultry diets. The objective of this study was to determine the effects of level of inclusion of wDDGS with or without enzyme (E-, i.e., wDDGSE-) supplementation on performance and water consumption of turkey hens (0 to 72 d). Two diets (0 or 30% wDDGS) were formulated to meet the nutrient requirements of Hybrid Converter turkeys. Diets (0 or 30% wDDGS; starter, grower, and finisher) were then blended to obtain a different level of inclusion (15%) of wDDGS. The 30% wDDGS diet was divided into 3 fractions and 2 fractions supplemented with either protease (P+, i.e., wDDGSP+; 0.126 g/kg) or ß-mannanase (M+, i.e., wDDGSM+; 0.05 g/kg). All 5 diets were fed ad libitum as mash. The 700 0-d turkey hens were randomly allocated into groups of 35 birds per replicate with 4 replicate floor pens per treatment, in a completely randomized design. Water consumption per pen was recorded beginning at 7 d. There was no effect of dietary treatment on feed intake. BW of turkey hens (52 d; grower) was significantly higher for 30% wDDGSP+ as compared to 0% wDDGSE- or 15% wDDGSE- diets; but was not different from 30% wDDGSE- or 30% wDDGSM+ diets. FCR (P < 0.01; 28 to 52 d), and total FCR (P < 0.05; 0 to 72 d) was significantly improved for birds fed 15 or 30% wDDGS regardless of enzyme treatment compared to 0% wDDGSE-. Water intake (WI, in mL per bird per day) tended to be higher (P = 0.08) between 7 and 28 d for 30% wDDGSP+ diets compared to other treatments. Similarly, WI of birds fed 30% wDDGSP+ was higher (P < 0.05; 28 to 52 and 52 to 72 d) and total WI (P = 0.07; 7 to 72 d) tended to be higher than other treatments. This study is the first to report the impact of wDDGS on WI. As high as 30% wDDGS can be substituted in turkey hen diets. No effect of P+ or M+ at the inclusion level tested was found on performance.

ß-Mannanase production by Aspergillus niger BCC4525 and its efficacy on broiler performance.

BACKGROUND: Mannan is a hemicellulose constituent commonly found in plant-derived feed ingredients. The gum-like property of mannan can obstruct digestive enzymes and bile acids, resulting in impaired nutrient utilisation. In this study, ß-mannanase production by Aspergillus niger strain BCC4525 was investigated using several agricultural residues under solid state condition. The biochemical properties of the target enzyme and the effects of enzyme supplementation on broiler performance and energy utilisation were assessed. RESULTS: Among five carbon sources tested, copra meal was found to be the best carbon source for ß-mannanase production with the maximum yield of 1837.5 U g(-1) . The crude ß-mannanase exhibited maximum activity at 80 °C within a broad range of pH from 2 to 6. In vitro digestibility assay, which simulates the gastrointestinal tract system of broilers, showed that ß-mannanase could liberate reducing sugars from corn/soybean diet. Surprisingly, ß-mannanase supplementation had no significant effect on broiler feed intake, feed conversion rate or energy utilisation. CONCLUSION: A high level of ß-mannanase was produced by A. niger BCC4525 under solid state condition using copra meal as carbon source. Although the enzyme has the desired properties of an enzyme additive for improving broiler performance, it does not appear to be beneficial.

Lipidemic responses of male broiler chickens to enzyme-supplemented wheat-soybean meal-based diets with various levels of metabolizable energy.

Effects of 2 various levels of AME (according to the manual recommendation and 100 kcal kg(-1) less than it), 2 levels of endo-beta-D-mannanase enzyme (0, 1 g kg(-1)) and 2 levels of xylanase enzyme (0 and 1 g kg(-1)) on serum lipid parameters as a 2(3) factorial arrangement were tested in 120 male broiler chicks fed wheat-soybean meal-based diet. These birds were randomly assigned to 8 experimental groups with 3 pen per group and 5 birds per pen. The serum HDL-cholesterol (HDL), LDL-cholesterol (LDL), Total-cholesterol (TC) and Triglycerides (TG) concentrations were measured at 31 and 41 day of age. The concentrations of serum TG, TC and LDL of 41-day-old birds demonstrated to be lower than those of 31-d-old (p < 0.001). Some hypolipidemic responses were observed in the broiler chicks fed on (1) Diet supplemented with only beta-mannanase, (2) Normal-AME diets supplemented with p-mannanase, (3) Normal-AME diets supplemented with Xylanse and (4) Normal-AME diets supplemented with both beta-mannanase and Xylanase (p < 0.01). In the other hand, some hyperlipidemic responses were detected in the broiler chicks fed on low-AME diets supplemented with xylanse or beta-mannanase enzymes, alone or in combination (p < 0.01). Regardless of AME, adding both xylanse and beta-mannanase to the wheat-soybean meal-based diets have both hyperlipidemic and hypolipidemic effects together (p < 0.01).

Effects of guar meal by-product with and without beta-mannanase Hemicell on broiler performance.

Guar gum production yields a high protein guar meal that can be subdivided into germ and hull fractions. Feeding high concentrations of guar meal reduces body weight and feed efficiency in chickens due to the presence of a residual guar gum. Two experiments determined the upper feeding levels of guar meal and the hull and germ fractions in broiler chickens. An industrial source beta-mannanase (Hemicell) also was fed in combination with guar meals. Experiment 1 utilized a 3 x 4 factorial design to feed broiler chickens diets containing guar germ, guar hull, or guar meal at 4 levels (2.5, 5.0, 7.5, and 10.0%) compared with a negative control diet. Results indicated that any of the 3 guar meals could be fed at a 2.5% dietary inclusion rate without adversely affecting broiler chicken growth to 6 wk of age. In experiment 2, a 4 x 2 factorial design consisting of the 3 by-products meals at 5% inclusion and soybean meal control with and without enzyme tested whether Hemicell could increase inclusion rates without decreasing broiler growth or feed consumption to 6 wk of age. Addition of Hemicell to feed had no effect on measures of growth in chickens fed the control diet. Hemicell significantly improved feed:gain ratio of diets containing 5% of each fraction of guar meal versus the untreated diets. Feed:gain ratio for the Hemicell-treated 5% germ fraction diet was improved to control diet levels. Results indicated that the upper feeding level of guar meal and germ and hull fraction of guar meal is 2.5%, and addition of beta-mannanase (Hemicell) increases the upper feeding level for the germ fraction to 5%.

Effects of beta-mannanase in corn-soy diets on commercial leghorns in second-cycle hens.

beta-Mannanase (Hemicell) is a unique enzyme-based feed ingredient that can hydrolyze beta-mannan, an antinutritional fiber in feed. Because soybean meal contains beta-mannan and its derivatives, addition of beta-mannanase may improve soybean-meal utilization. The purpose of this study was to evaluate the effect of beta-mannanase on performance of commercial Leghorns fed corn-soybean meal based diets. In this experiment, 3 diets were formulated. The metabolizable energy content for diet 1 (high-energy diet) was 2,951 kcal/kg, which was 120 kcal/kg higher than diet 2 (low-energy diet supplemented with beta-mannanase) and diet 3 (low-energy diet without beta-mannanase). Hy-Line W-36 hens (n = 720, 98 wk old) were randomly divided into 3 dietary treatments (16 replicates of 15 hens per treatment). The trial lasted for 12 wk. Overall average feed conversion of hens fed the low-energy diet supplemented with beta-mannanase was similar to that of hens fed the high-energy diet, and both were significantly lower than that of hens fed the low-energy diet without beta-mannanase. There were no significant differences in overall average egg production and egg mass among 3 dietary treatments for the 12-wk period. However, the addition of beta-mannanase significantly increased average egg production and egg mass of hens fed the low-energy diet from wk 5 to 8. There were no significant differences in feed intake, egg specific gravity, egg weight, mortality, body weight, and body weight variability among the 3 dietary treatments. beta-Mannanase supplementation improved energy utilization of corn-soybean layer diets and has potential to reduce the cost of practical laying hen diets containing beta-mannan.

A dose-response study with the feed enzyme beta-mannanase in broilers provided with corn-soybean meal based diets in the absence of antibiotic growth promoters.

An experiment was designed to assess the effects of graded levels of beta-mannanase on performance and body weight uniformity of male broilers provided with diets based on corn and soybean meal and devoid of antibiotic growth promoters or coccidiostats. Four dietary treatments contained 0, 50, 80, and 110 MU of Hemicell/ ton (where 1 MU = 10(6) enzyme activity units, 100 MU/ton is manufacturer's recommendation). Each treatment contained 15 pens with 40 birds/pen. Individual bird weights were determined on d 0, 21, and 42. From 21 to 42 d of age, feed intake for the 80 MU/ton treatment was significantly greater than the 50 MU/ton treatment. beta-Mannanase inclusion at 80 or 110 MU/ton induced improvements (P < 0.05) in weight gain (3.9 to 4.8%) and feed efficiency (3.5 to 3.8%) over the control, whereas inclusion of 50 MU/ton resulted in no significant benefit. There were no significant differences between 80 or 110 MU/ton. The experiment demonstrated that dietary inclusion of beta-mannanase at approximately 50 MU/ton is not sufficient for maximum response. Inclusion at 80 MU/ ton improved broiler gains and feed conversion and increasing to 110 MU/ton resulted in no significant additional response.

Use of carbohydrases in corn-soybean meal-based nursery diets.

Three experiments were conducted to test the hypothesis that supplementing nursery pig diets with a mixture of carbohydrases (CS) will improve pig performance and nutrient digestibility. The CS used in these experiments contained 7 units/g of alpha-1,6-galactosidase, 22 units/g of beta-1,4-mannanase, beta-1,4 mannosidase, and trace amounts of other enzymes. In Exp. 1, 108 pigs weaned at d 21 of age were fed one of three diets containing 0 (control), 0.1, or 0.2% CS for 5 wk, based on a three-phase feeding program (1, 2, and 2 wk). Over the entire 35-d period, ADG was not affected (P > 0.05) by treatment, but supplementing 0.1% CS increased (P < 0.05) gain:feed by 9%. Experiment 2 used 10 gilts fitted with simple T-cannula in the terminal ileum at 3 wk of age. After cannulation, pigs were fed the same control Phase I and II diets, but the Phase III diet contained either 0 or 0.1% CS. Ileal samples were collected for the 3 d following the 5-d adjustment period during Phase III. Apparent ileal digestibility of GE, lysine, threonine, and tryptophan was greater (P < 0.05) in the CS diet. In Exp. 3, 90 pigs weaned at 21 d of age were fed the same control Phase I and II diets, but the Phase III diet contained either 0 or 0.1% CS. Phase III diets were fed for 3 wk. Average daily gain of the CS group was greater (P < 0.05) than the control group during wk 3. Gain:feed ratio was greater (P < 0.05) for the carbohydrase group during the entire Phase III period. Four pigs per treatment were killed at the end of Exp. 3 to measure villus height and to determine the concentration of raffinose and stachyose in different parts of the gastrointestinal tract. Average villus height was greater (P < 0.05) in pigs fed the CS diet. Carbohydrase supplementation decreased (P < 0.05) the concentration of stachyose in freeze-dried digesta from the proximal and distal small intestine. Raffinose concentration, on the other hand, was decreased (P < 0.05) by CS supplementation only in the distal small intestine. These lower concentrations suggest that CS improved the digestibility of carbohydrate in soybean meal. In conclusion, the addition of CS to Phase I and Phase II nursery diets containing low levels of soybean meal did not improve pig performance, but its addition to corn-soybean meal-based Phase III nursery diets improved gain:feed ratio and energy and AA digestibility.

Beneficial effect of beta-mannanase feed enzyme on performance of chicks challenged with Eimerla sp. and Clostridium perfringens.

Two experiments were conducted to determine the effect of a beta-mannanase feed enzyme on the performance of broiler chicks subject to a necrotic enteritis disease challenge model involving oral inoculation of Eimeria sp. and Clostridium pefringens. Beta-mannanase is known to improve productive performance when added to poultry and swine diets. In both experiments, disease challenge in the absence of feed additives demonstrated significant reductions in performance as measured by weight gain, feed conversion, and the incidence of coccidial lesion scores. Significant mortality was also observed in challenged groups in Experiment 1. The disease challenge model was therefore judged as highly effective. Additions of a commonly used antibiotic, bacitracin methylene disalicilate (BMD), and coccidiostat, salinomycin, were highly effective in partially counteracting negative effects of the disease challenge. In both experiments, addition of beta-mannanase significantly improved performance and reduced lesion scores in disease-challenged groups. The degree of improvement was somewhat less than that afforded by a combination of BMD and salinomycin in Experiment 1 but was not different from that afforded by BMD alone in Experiment 2. We conclude that the beta-mannanase enzyme can play a role in circumstances where the use of antibiotics is not desired.

beta-Mannanase ameliorates viscosity-associated depression of growth in broiler chickens fed guar germ and hull fractions.

High concentrations of guar meal in broiler chicken diets reduce body weight and feed efficiency. The increased intestinal viscosity that is responsible for reduced measures of performance results from residual guar gum present in guar meal. Two experiments were designed to study the effects of 2 guar meal fractions at 3 different concentrations, germ (0, 5.0, and 7.5%) and hull (0, 2.5, and 5.0%), and the effectiveness of a beta-mannanase at three levels (0, 1x, and 4x; 1x = 1.09 x 10(5) units/ kg) on broiler growth and feed conversion. Growth and performance were measured as a function of intestinal viscosity. Addition of the germ fraction to rations did not reduce body weight, although feed conversion ratio was increased at 7.5% of the diet. Intestinal viscosity also increased significantly at this level. Enzyme addition significantly reduced intestinal viscosity. Due to an interaction that was present between hull and enzyme concentration, each treatment was compared separately. Inclusion of the hull fraction significantly reduced body weight at both levels of inclusion and increased feed conversion ratio at 5% inclusion. Addition of the enzyme significantly increased body weight and reduced feed conversion ratio in diets containing guar hull fractions. Supplementation of beta-mannanase to feeds containing either fraction of guar meal reduced intestinal viscosity and alleviated the deleterious effects associated with guar meal feeding.