Zika detection: comparison of methodologies
Braz. j. microbiol
; Braz. j. microbiol;49(1): 144-147, Jan.-Mar. 2018. tab, graf
Article
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| LILACS
| ID: biblio-889187
Biblioteca responsable:
BR1.1
ABSTRACT
ABSTRACT Many countries in the Americas have detected local transmission of multiple arboviruses that cause febrile illnesses. Therefore, laboratory testing has become an important tool for confirming the etiology of these diseases. The present study aimed to compare the sensitivity and specificity of three different Zika virus detection assays. One hundred serum samples from patients presenting with acute febrile symptoms were tested using a previously reported TaqMan® RT-qPCR assay. We used a SYBR® Green RT-qPCR and a conventional PCR methodologies to compare the results. Of the samples that were determined to be negative by the TaqMan® RT-qPCR assay, 100% (Kappa = 0.670) were also found to be negative by SYBR® Green RT-qPCR based on Tm comparison; however, 14% (Kappa = 0.035) were found to be positive by conventional PCR followed by agarose gel electrophoresis. The differences between the ZIKV strains circulating worldwide and the low viremia period can compromise diagnostic accuracy and thereby the accuracy of outbreak data. Therefore, improved assays are required to improve the diagnosis and surveillance of arbovirus.
Palabras clave
Texto completo:
1
Base de datos:
LILACS
Asunto principal:
Reacción en Cadena de la Polimerasa
/
Virus Zika
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Infección por el Virus Zika
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
Braz. j. microbiol
Asunto de la revista:
MICROBIOLOGIA
Año:
2018
Tipo del documento:
Article
/
Project document
País de afiliación:
Brasil