The normal chain length distribution of the O antigen is required for the interaction of Shigella flexneri 2a with polarized Caco-2 cells
Biol. Res
; 45(1): 21-26, 2012. ilus, tab
Article
en En
| LILACS
| ID: lil-626743
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ABSTRACT
Shigella flexneri causes bacillary dysentery in humans. Essential to the establishment of the disease is the invasion of the colonic epithelial cells. Here we investigated the role of the lipopolysaccharide (LPS) O antigen in the ability of S. flexneri to adhere to and invade polarized Caco-2 cells. The S. flexneri 2a O antigen has two preferred chain lengths a short O antigen (S-OAg) regulated by the WzzB protein and a very long O antigen (VL-OAg) regulated by Wzz pHS2. Mutants with defined deletions of the genes required for O-antigen assembly and polymerization were constructed and assayed for their abilities to adhere to and enter cultured epithelial cells. The results show that both VL- and S-OAg are required for invasion through the basolateral cell membrane. In contrast, the absence of O antigen does not impair adhesion. Purified LPS does not act as a competitor for the invasion of Caco-2 cells by the wild-type strain, suggesting that LPS is not directly involved in the internalization process by epithelial cells.
Palabras clave
Texto completo:
1
Base de datos:
LILACS
Asunto principal:
Shigella flexneri
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Proteínas Bacterianas
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Adhesión Bacteriana
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Antígenos O
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Disentería Bacilar
Límite:
Humans
Idioma:
En
Revista:
Biol. Res
Asunto de la revista:
BIOLOGIA
Año:
2012
Tipo del documento:
Article
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Project document
País de afiliación:
Canadá
/
Chile