Orientational behavior of phospholipid membranes with mastoparan studied by 31P solid state NMR.
FEBS Lett
; 455(3): 228-32, 1999 Jul 23.
Article
en En
| MEDLINE
| ID: mdl-10437778
ABSTRACT
Solid state 31P NMR spectroscopy was used to study the perturbing effect of the wasp venom peptide mastoparan (MP) on lipid bilayers composed of dimyristoylphosphatidylcholine (DMPC) and dimyristoylphosphatidylglycerol (DMPG). The 31P chemical shift anisotropy of multilamellar vesicles decreased with increasing peptide concentration, indicating that MP interacts strongly and selectively with the charged DMPG head group. Macroscopically oriented MP-lipid samples between glass plates were studied by 31P NMR as a function of tilt angle. These spectra showed the coexistence of orientation-dependent lamellar signals as well as an isotropic peak, suggesting that MP can induce non-lamellar phases in DMPC/DMPG membranes.
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Base de datos:
MEDLINE
Asunto principal:
Venenos de Avispas
/
Membrana Dobles de Lípidos
Límite:
Animals
Idioma:
En
Revista:
FEBS Lett
Año:
1999
Tipo del documento:
Article
País de afiliación:
Japón