Determination of a new naphthoquinone derivative, NQ12, in human plasma and urine by high-performance liquid chromatography.
Res Commun Mol Pathol Pharmacol
; 103(1): 101-7, 1999 Jan.
Article
en En
| MEDLINE
| ID: mdl-10440575
ABSTRACT
A high-performance liquid chromatographic method was developed for the determination of a new phospholipase A2 inhibitor, NQ12, in human plasma and urine. The sample preparation was simple 2 volumes of acetonitrile were added to the biological samples to deproteinize it. A 50-microl aliquot of the supernatant was injected onto a C18 reversed-phase column. The mobile phase employed was 0.05M acetate buffer (pH 3) acetonitrile methanol (304525, v/v/v) and run at a flow rate of 1.5 ml/min. The column effluent was monitored by a UV detector set at 298 nm. The retention times for NQ12 and the internal standard were approximately 5.5 and 7.0 min, respectively. The detection limits for NQ12 in human plasma and urine were all 20 ng/ml. The coefficients of variation of the assay (within-day and between-day) were generally low (below 11.8%) for human plasma and urine. No interferences from endogenous substances were found.
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Base de datos:
MEDLINE
Asunto principal:
Inhibidores Enzimáticos
/
Naftalenos
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
Res Commun Mol Pathol Pharmacol
Asunto de la revista:
BIOLOGIA MOLECULAR
/
FARMACOLOGIA
/
PATOLOGIA
Año:
1999
Tipo del documento:
Article
País de afiliación:
Corea del Sur