PKC-beta and PKC-zeta mediate opposing effects on proximal tubule Na+,K+-ATPase activity.
FEBS Lett
; 456(1): 45-8, 1999 Jul 30.
Article
en En
| MEDLINE
| ID: mdl-10452527
ABSTRACT
Dopamine (DA) inhibits rodent proximal tubule Na+,K+-ATPase via stimulation of protein kinase C (PKC). However, direct stimulation of PKC by phorbol 12-myristate 13-acetate (PMA) results in increased Na+,K+-ATPase. LY333531, a specific inhibitor of the PKC-beta isoform, prevents PMA-dependent activation of Na+,K+-ATPase, but has no effect on DA inhibition of this activity. A similar result was obtained with a PKC-beta inhibitor peptide. Concentrations of staurosporine, that inhibits PKC-zeta, prevent DA-dependent inhibition of Na+,K+-ATPase and a similar effect was obtained with a PKC-zeta inhibitor peptide. Thus, PMA-dependent stimulation of Na+,K+-ATPase is mediated by activation of PKC-beta, whereas inhibition by DA requires activation of PKC-zeta.
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Base de datos:
MEDLINE
Asunto principal:
Proteína Quinasa C
/
ATPasa Intercambiadora de Sodio-Potasio
/
Isoenzimas
/
Túbulos Renales Proximales
Límite:
Animals
Idioma:
En
Revista:
FEBS Lett
Año:
1999
Tipo del documento:
Article
País de afiliación:
Estados Unidos