Suitability of MDCK cells grown in a serum-free medium for influenza virus production.
Dev Biol Stand
; 98: 13-21; discussion 73-4, 1999.
Article
en En
| MEDLINE
| ID: mdl-10494956
ABSTRACT
MDCK cells have been adapted to grow in a serum-free environment using Ultra-MDCK medium (BioWHITTAKER). The growth of adapted U-MDCK cells was maintained for over a year without any reduction in growth rate or modification of cell karyotype; cells were scaled up to spinner culture using several microcarriers. The cells were shown to be a very good host for influenza A and B virus replication in both the presence and absence of trypsin in the infection medium. Trypsin-independent viruses replicated to high titres (10(7)-10(8) TCID50/ml) in U-MDCK cells, after selection through serial passages without trypsin. This virus progeny exhibited uncleaved and antigenically modified haemagglutinin compared with standard viruses grown with trypsin. Finally, large amounts of influenza A and B viruses were produced in U-MDCK cells grown on microcarriers under rod-stirred conditions using selected trypsin-independent variants.
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Base de datos:
MEDLINE
Asunto principal:
Virus de la Influenza A
/
Virus de la Influenza B
/
Cultivo de Virus
/
Línea Celular
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Dev Biol Stand
Año:
1999
Tipo del documento:
Article