Introduction of 6-formylcytidine into a Myb binding sequence.
Nucleosides Nucleotides
; 18(11-12): 2769-83, 1999.
Article
en En
| MEDLINE
| ID: mdl-10639756
ABSTRACT
Single 6-formylcytidine was introduced into a oligonucleotide duplex (23 mers) as a substitute for thymidine in the Myb binding sequence of 3'-TTGAC-5'. The modified duplex showed Tm of 67 degrees C, which was six degrees lower than the Tm of the native duplex. Binding affinity of the 23-mers to the Myb protein was estimated by electrophoretic mobility shift assays, and the binding was almost completely abolished.
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Base de datos:
MEDLINE
Asunto principal:
Oligodesoxirribonucleótidos
/
Proteínas Proto-Oncogénicas c-myb
/
Citidina
Límite:
Humans
Idioma:
En
Revista:
Nucleosides Nucleotides
Año:
1999
Tipo del documento:
Article
País de afiliación:
Japón