Effects in live cells of a c-myc anti-gene PNA linked to a nuclear localization signal.
Nat Biotechnol
; 18(3): 300-3, 2000 Mar.
Article
en En
| MEDLINE
| ID: mdl-10700145
ABSTRACT
Peptide nucleic acids (PNA) are synthetic homologs of nucleic acids in which the phosphate-sugar polynucleotide backbone is replaced by a flexible polyamide. In this study, a PNA construct was employed as an anti-gene agent in intact cells in culture. The cell lines studied were derived from Burkitt's lymphomas (BL) that presented a translocated and hyperexpressed c-myc oncogene. A 17-mer anti-myc PNA, complementary to a unique sequence located at the beginning of the second exon of the oncogene, and was covalently linked at its N terminus to a nuclear localization signal (NLS) (PNA-myc(wt)-NLS). When BL cells were exposed to PNA-myc(wt)-NLS, the anti-gene construct was localized predominantly in the cell nuclei and a rapid consequent downregulation of c-myc expression occurred. Under these conditions, both completion of a productive cell cycle and apoptosis were inhibited.
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Base de datos:
MEDLINE
Asunto principal:
Genes myc
/
Señales de Localización Nuclear
/
Ácidos Nucleicos de Péptidos
Límite:
Humans
Idioma:
En
Revista:
Nat Biotechnol
Asunto de la revista:
BIOTECNOLOGIA
Año:
2000
Tipo del documento:
Article
País de afiliación:
Italia