Accumulation of a form of p27(Kip1) not associated with Cdk-cyclin complexes in transforming growth factor-beta-arrested Mv1Lu cells.
Exp Cell Res
; 259(1): 107-16, 2000 Aug 25.
Article
en En
| MEDLINE
| ID: mdl-10942583
The p27(Kip1) cyclin-dependent kinase inhibitor translocates in response to transforming growth factor-beta to a Cdk2-cyclin E complex inhibiting its catalytic activity, but the p27(Kip1) protein levels are unaffected [1]. We show here that transforming growth factor-beta induces the accumulation of a form of p27(Kip1) representing a subpopulation of total p27(Kip1) in growth-arrested Mv1Lu epithelial cells. The inducible p27(Kip1) is detectable only by a specific p27(Kip1) monoclonal antibody recognizing a native form of p27(Kip1). The increase in this subset of p27(Kip1) correlates with G(1) arrest and withdrawal of the cells from the cycle induced by transforming growth factor-beta, serum starvation, or contact inhibition. In contrast to the majority of p27(Kip1) in the cells, the transforming growth factor-beta-inducible p27(Kip1) is devoid of cyclin-dependent kinase/cyclin interactions. The results indicate that growth arresting treatments induce the accumulation of non-cyclin-dependent kinase-bound p27(Kip1), which may function as a reservoir for inhibition of Cdk2-cyclin E activities.
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Base de datos:
MEDLINE
Asunto principal:
Factor de Crecimiento Transformador beta
/
Proteínas Proto-Oncogénicas
/
Proteínas Serina-Treonina Quinasas
/
Quinasas Ciclina-Dependientes
/
Proteínas de Ciclo Celular
/
Ciclina E
/
Proteínas Supresoras de Tumor
/
Quinasas CDC2-CDC28
/
Células Epiteliales
/
Proteínas Asociadas a Microtúbulos
Tipo de estudio:
Risk_factors_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Exp Cell Res
Año:
2000
Tipo del documento:
Article
País de afiliación:
Finlandia