A deficiency in DNA repair and DNA-PKcs expression in the radiosensitive BALB/c mouse.
Cancer Res
; 60(16): 4342-5, 2000 Aug 15.
Article
en En
| MEDLINE
| ID: mdl-10969773
ABSTRACT
We have studied the efficiency of DNA double strand break (DSB) rejoining in primary cells from mouse strains that show large differences in in vivo radiosensitivity and tumor susceptibility. Cells from radiosensitive, cancer-prone BALB/c mice showed inefficient end joining of gamma ray-induced DSBs as compared with cells from all of the other commonly used strains and F1 hybrids of C57BL/6 and BALB/c mice. The BALB/c repair phenotype was accompanied by a significantly reduced expression level of DNA-PKcs protein as well as a lowered DNA-PK activity level as compared with the other strains. In conjunction with published reports, these data suggest that natural genetic variation in nonhomologous end joining processes may have a significant impact on the in vivo radiation response of mice.
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Base de datos:
MEDLINE
Asunto principal:
Tolerancia a Radiación
/
Proteínas Serina-Treonina Quinasas
/
Proteínas de Unión al ADN
/
Reparación del ADN
Límite:
Animals
Idioma:
En
Revista:
Cancer Res
Año:
2000
Tipo del documento:
Article
País de afiliación:
Estados Unidos