Expression of interleukin-6 receptor, leukemia inhibitory factor receptor and glycoprotein 130 in the murine cerebellum and neuropathological effect of leukemia inhibitory factor on cerebellar Purkinje cells.
Neuroscience
; 100(4): 841-8, 2000.
Article
en En
| MEDLINE
| ID: mdl-11036218
ABSTRACT
Expression of glycoprotein 130 and the related receptors, including interleukin-6 receptor and leukemia inhibitory factor receptor, was examined in the murine cerebellum at the protein level. Western blot analysis revealed that interleukin-6 receptor, leukemia inhibitory factor receptor and glycoprotein 130 were expressed in the murine cerebellum. Immunoreactivities for interleukin-6 receptor, leukemia inhibitory factor receptor and glycoprotein 130 were strongly localized on the cell body of Purkinje cells, indicating that both interleukin-6 and leukemia inhibitory factor could act directly on Purkinje cells in murine adult mice. The expressions of interleukin-6 receptor, leukemia inhibitory factor receptor and glycoprotein 130 were observed on the cell membranes of Purkinje cells by immunoelectron microscopy. Immunoreactivity for the interleukin-6 receptor was also detected in the cytoplasm of Purkinje cells. Injection of a murine hemopoietic cell line, FDC-P1 cells, transfected with the complementary DNA encoding the leukemia inhibitory factor led to a reduction in calbindin-positive dendrites of the Purkinje cells.The present results suggest that the leukemia inhibitory factor affects cerebellar functions through Purkinje cells.
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Base de datos:
MEDLINE
Asunto principal:
Células de Purkinje
/
Glicoproteínas de Membrana
/
Cerebelo
/
Linfocinas
/
Interleucina-6
/
Receptores de Citocinas
/
Receptores de Interleucina-6
/
Inhibidores de Crecimiento
Límite:
Animals
Idioma:
En
Revista:
Neuroscience
Año:
2000
Tipo del documento:
Article
País de afiliación:
Japón