Influence of methylenetetrahydrofolate reductase genotype, age, vitamin B-12, and folate status on plasma homocysteine in children.
Am J Clin Nutr
; 72(6): 1469-73, 2000 Dec.
Article
en En
| MEDLINE
| ID: mdl-11101473
ABSTRACT
BACKGROUND:
Several studies have examined the association of the methylenetetrahydrofolate reductase (MTHFR) genotype with plasma homocysteine in adults, but few studies have been performed in children.OBJECTIVE:
We measured the concentrations of plasma total homocysteine, folate, and vitamin B-12 in a group of healthy fasting children and related these to MTHFR genotype.DESIGN:
After the subjects fasted, blood samples were collected into EDTA-containing tubes. Plasma, red blood cells, and the buffy coat were immediately stored at -80 degrees C for biochemical and molecular analyses. Plasma total homocysteine was determined by HPLC. Folate and vitamin B-12 were measured by a double-labeled radioimmunoassay, and the genotypic analysis was performed by polymerase chain reaction amplification of genomic DNA extracted from blood leukocytes.RESULTS:
Plasma homocysteine concentrations correlated negatively with folate and vitamin B-12(,) but positively with age (P < 0. 0001). Whereas folate and vitamin B-12 accounted for 27% and 19% of the variation in homocysteine, respectively, age accounted for 48% of the variation. When the cohort was divided into older (>10 y) and younger (=10 y) individuals, folate was significantly lower in the older individuals who were homozygous for the mutation (T/T) than in those who were homozygous for the wild-type allele (C/C). Homocysteine was higher in the T/T group than in both the C/C and C/T subgroups aged >10 y.CONCLUSION:
Our data show that in a healthy pediatric population, MTHFR genotype played a significant role in determining homocysteine concentrations in older (>10 y), nutritionally stressed children.
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Base de datos:
MEDLINE
Asunto principal:
Vitamina B 12
/
Envejecimiento
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Ácido Fólico
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Oxidorreductasas actuantes sobre Donantes de Grupo CH-NH
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Homocisteína
Límite:
Adolescent
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Child
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Child, preschool
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Female
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Humans
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Male
Idioma:
En
Revista:
Am J Clin Nutr
Año:
2000
Tipo del documento:
Article
País de afiliación:
Canadá