Phylogenetic analysis of the rompB genes of Rickettsia felis and Rickettsia prowazekii European-human and North American flying-squirrel strains.
Am J Trop Med Hyg
; 62(5): 598-603, 2000 May.
Article
en En
| MEDLINE
| ID: mdl-11289671
ABSTRACT
The rickettsial outer membrane protein B (rompB) gene encodes the major surface antigens of Rickettsia species. We undertook sequencing and molecular analysis of the rompB gene of Rickettsia felis and a comparison with its homologs in spotted fever group (SFG) and typhus group (TG) rickettsiae, including the complete sequences of two North American flying squirrel strains and two European human strains of Rickettsia prowazekii. We sequenced 5,226 base pairs (bp) of the R. felis rompB, encoding a protein of 1,654 amino acids. We also sequenced 5,015 bp of rompB of the flying squirrel strains, encoding a protein of 1,643 amino acids. Analysis of the R. felis rompB gene sequence showed 10-13% divergence from SFG rickettsiae and 18% divergence from the TG rickettsiae. The rompB of all sequenced strains of R. prowazekii showed an overall similarity of 99.7-99.9%.
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Base de datos:
MEDLINE
Asunto principal:
Filogenia
/
Rickettsia
/
Rickettsia prowazekii
/
Proteínas de la Membrana Bacteriana Externa
/
Genes Bacterianos
/
Antígenos Bacterianos
/
Antígenos de Superficie
Límite:
Animals
/
Humans
País/Región como asunto:
America do norte
/
Europa
Idioma:
En
Revista:
Am J Trop Med Hyg
Año:
2000
Tipo del documento:
Article
País de afiliación:
Estados Unidos