Characterization of the major allergen of plum as a lipid transfer protein.
J Chromatogr B Biomed Sci Appl
; 756(1-2): 95-103, 2001 May 25.
Article
en En
| MEDLINE
| ID: mdl-11419731
ABSTRACT
BACKGROUND:
Allergy to Prunoideae fruit (plum, peach, cherry and apricot) is one of the most frequent food allergies in southern Europe. All these fruits cross-react in vivo and in vitro, as they share their major allergen, a 9 kD lipid transfer protein (LTP).OBJECTIVE:
The aim of the study was the identification and molecular characterization of the major allergen of plum.METHODS:
The IgE pattern of reactivity to plums was investigated by SDS-PAGE and immunoblotting with the sera of 23 patients. The identified major allergen was purified by HPLC, using a cationic-exchange column followed by gel-filtration. Further characterization was achieved by periodic-Schiff stain, isoelectrofocusing and N-terminal amino acid sequencing. RESULTS ANDCONCLUSIONS:
The major allergen of plum is a 9 kD lipid transfer protein, not glycosylated and with a basic character (pI>9), highly homologous to the major allergen of peach.
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Base de datos:
MEDLINE
Asunto principal:
Alérgenos
/
Proteínas Portadoras
/
Frutas
Límite:
Adolescent
/
Adult
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
J Chromatogr B Biomed Sci Appl
Asunto de la revista:
QUIMICA CLINICA
Año:
2001
Tipo del documento:
Article
País de afiliación:
Italia