Infected splenic dendritic cells are sufficient for prion transmission to the CNS in mouse scrapie.
J Clin Invest
; 108(5): 703-8, 2001 Sep.
Article
en En
| MEDLINE
| ID: mdl-11544275
ABSTRACT
Transmissible spongiform encephalopathies display long incubation periods at the beginning of which the titer of infectious agents (prions) increases in peripheral lymphoid organs. This "replication" leads to a progressive invasion of the CNS. Follicular dendritic cells appear to support prion replication in lymphoid follicles. However, the subsequent steps of neuroinvasion remain obscure. CD11c(+) dendritic cells, an unrelated cell type, are candidate vectors for prion propagation. We found a high infectivity titer in splenic dendritic cells from prion-infected mice, suggesting that dendritic cells carry infection. To test this hypothesis, we injected RAG-1(0/0) mice intravenously with live spleen cell subsets from scrapie-infected donors. Injection of infected dendritic cells induced scrapie without accumulation of prions in the spleen. These results suggest that CD11c(+) dendritic cells can propagate prions from the periphery to the CNS in the absence of any additional lymphoid element.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Scrapie
/
Bazo
/
Células Dendríticas
/
Priones
Límite:
Animals
Idioma:
En
Revista:
J Clin Invest
Año:
2001
Tipo del documento:
Article
País de afiliación:
Francia