Mutation and abnormal expression of the fragile histidine triad gene in nasopharyngeal carcinoma.
Laryngoscope
; 111(9): 1589-92, 2001 Sep.
Article
en En
| MEDLINE
| ID: mdl-11568610
ABSTRACT
OBJECTIVE:
To determine alterations of fragile histidine triad (FHIT) gene in nasopharyngeal carcinoma and the correlation of FHIT gene with nasopharyngeal carcinogenesis. STUDYDESIGN:
Prospective study.METHODS:
A total of 28 nasopharyngeal carcinoma and 16 normal nasopharyngeal epithelium specimens were examined for abnormalities of FHIT gene by nested reverse-transcriptase-polymerase chain reaction and DNA sequencing.RESULTS:
The deletion of FHIT gene was not observed in 16 normal nasopharyngeal epithelium specimens. In 28 cases of nasopharyngeal carcinoma tissues, 12 (42.9%) exhibited FHIT aberrant transcripts. Complementary DNA sequencing revealed exonic deletion, small DNA insertion, synonymous mutation in exon 8, or frameshift mutation in exon 5.CONCLUSIONS:
The present results suggest that the FHIT gene may play an important role in the pathogenesis of nasopharyngeal carcinoma and may be one of the candidate tumor suppressor genes in nasopharyngeal carcinoma.
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Base de datos:
MEDLINE
Asunto principal:
Proteínas
/
Regulación Neoplásica de la Expresión Génica
/
Neoplasias Nasofaríngeas
/
Fragilidad Cromosómica
/
Hidrolasas
/
Mutación
Tipo de estudio:
Observational_studies
/
Risk_factors_studies
Límite:
Humans
País/Región como asunto:
Asia
Idioma:
En
Revista:
Laryngoscope
Asunto de la revista:
OTORRINOLARINGOLOGIA
Año:
2001
Tipo del documento:
Article
País de afiliación:
China