Application of a Designed Synthetic Ligand for the Separation and Purification of Alkaline Phosphatase.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai)
; 30(3): 241-245, 1998.
Article
en En
| MEDLINE
| ID: mdl-12174254
ABSTRACT
An artificial affinity ligand comprising a p-aminobenzyl phosphoric acid as inhibitor was designed and coupled to an agarose support by chlorotriazine ring to separate and purify alkaline phosphatase from calf intestine. The optimal density of the ligand is 6 &mgr;mol/g adsorbent. After elution with sodium chloride and inorganic phosphoric acid a 300-fold purified enzymatic preparation was obtained in one step with a yield of 90%.
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Base de datos:
MEDLINE
Idioma:
En
Revista:
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai)
Año:
1998
Tipo del documento:
Article
País de afiliación:
China