Association of IL-10 polymorphism with severity of illness in community acquired pneumonia.
Thorax
; 58(2): 154-6, 2003 Feb.
Article
en En
| MEDLINE
| ID: mdl-12554901
ABSTRACT
BACKGROUND:
The influence of genetic polymorphisms of interleukin (IL)-10, tumour necrosis factor (TNF)-alpha, and IL-6 gene promoters on severity of systemic inflammatory response syndrome (SIRS) associated with community acquired pneumonia (CAP) was studied.METHODS:
Using PCR-RFLP analysis we analysed a -1082G/A single nucleotide polymorphism (SNP) of the anti-inflammatory IL-10 gene, a -308G/A SNP of the pro-inflammatory TNF-alpha gene and a -174G/C SNP of the IL-6 gene. Illness severity was stratified according to SIRS score, calculated by presence of up to four physiological indices temperature, white blood cell count, heart rate and respiratory rate (non-SIRS, SIRS 2, SIRS 3, and SIRS 4).RESULTS:
A statistically significant stepwise increase in frequency of the IL-10 G allele, associated with higher expression of the gene, was observed in patients with increasing severity of illness from non-SIRS (n=19) to SIRS 2 (n=17), SIRS 3 (n=33) and SIRS 4 (n=24). This was primarily due to a higher frequency of the GG genotype with increasing severity from non-SIRS through to SIRS 4. IL-10 G allele frequency was also increased in patients who died as a result of CAP (n=11) compared with CAP survivors (n=82) (p=0.01). No association was seen between the TNF-alpha -308G/A and IL-6 -174G/C SNPs and disease. Additionally, no interaction between all three SNP genotypes and disease severity was observed.CONCLUSIONS:
A polymorphism affecting IL-10 expression may influence the severity of illness in patients with CAP.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Neumonía
/
Polimorfismo Genético
/
Interleucina-10
Tipo de estudio:
Etiology_studies
/
Observational_studies
/
Risk_factors_studies
Límite:
Aged
/
Aged80
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Humans
Idioma:
En
Revista:
Thorax
Año:
2003
Tipo del documento:
Article
País de afiliación:
Irlanda