Increased sperm mitochondrial DNA content in male infertility.
Hum Reprod
; 18(3): 550-6, 2003 Mar.
Article
en En
| MEDLINE
| ID: mdl-12615823
ABSTRACT
BACKGROUND:
There is increasing evidence that mitochondrial DNA (mtDNA) anomalies in sperm may lead to infertility. Point mutations, deletions and the presence of a specific mtDNA haplogroup have been associated with poor sperm quality, but little attention has been paid to the role of mtDNA content.METHODS:
Using density gradient separation and swim-up methods, we selected motile sperm from 32 normal and 35 abnormal sperm samples. The mtDNA/beta-globin gene ratio was determined by real-time quantitative PCR.RESULTS:
The average mtDNA/beta-globin ratio of sperm collected from 100% density layers was 1.4 for normal sperm, 6.1 for sperm samples presenting at least one abnormal criterion [among the three criteria established by World Health Organization (1999), i.e. sperm count, motility and morphology], and 9.1 for sperm samples presenting two or more of these abnormal criteria. These differences are very highly significant (P < 0.0001). The mtDNA numbers were also much greater in sperm collected from the 40% density gradient layers (mean 17.1, P < 0.001), known to contain the most abnormal sperm of the sperm samples, than in those collected from the 100% layers known to contain sperm with the best fertilizing ability.CONCLUSION:
Our results showed significant mtDNA amplification in sperm collected from abnormal sperm samples.
Buscar en Google
Base de datos:
MEDLINE
Asunto principal:
Espermatozoides
/
ADN Mitocondrial
/
Infertilidad Masculina
Límite:
Humans
/
Male
Idioma:
En
Revista:
Hum Reprod
Asunto de la revista:
MEDICINA REPRODUTIVA
Año:
2003
Tipo del documento:
Article
País de afiliación:
Francia