Relationship between stallion sperm motility and viability as detected by two fluorescence staining techniques using flow cytometry.
Theriogenology
; 60(6): 1127-38, 2003 Oct 01.
Article
en En
| MEDLINE
| ID: mdl-12935852
ABSTRACT
Relationships between sperm motility parameters and viability were evaluated using two fluorescent staining techniques in fresh extended semen (fresh and after 24 h storage at 5 degrees C) that had various concentrations of dead sperm added to simulate different levels of viable and nonviable sperm. Both protocols incorporated SYBR-14 and propidium iodide (PI) while the second protocol added the mitochondrial probe JC-1. The relationship between total sperm motility and percent viable sperm was high between staining protocols (r = 0.98). Time (0 h versus 24 h, P<0.0001) and treatment (0, 10, 25, 50, and 75% nonviable sperm, P<0.0001) affected percent total sperm motility and percent viable sperm for both staining protocols. Actual percent viable sperm for each time and treatment did not differ from expected values.
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Base de datos:
MEDLINE
Asunto principal:
Motilidad Espermática
/
Espermatozoides
/
Citometría de Flujo
/
Colorantes Fluorescentes
/
Caballos
Tipo de estudio:
Guideline
Límite:
Animals
Idioma:
En
Revista:
Theriogenology
Año:
2003
Tipo del documento:
Article
País de afiliación:
Estados Unidos