Deficiency of inducible nitric oxide synthase exacerbates hepatic fibrosis in mice fed high-fat diet.
Biochem Biophys Res Commun
; 326(1): 45-51, 2005 Jan 07.
Article
en En
| MEDLINE
| ID: mdl-15567150
ABSTRACT
The role of inducible nitric oxide synthase (iNOS) in the progression of fibrosis during nonalcoholic steatohepatitis remains to be elucidated. This study examined the role of iNOS in the progression of fibrosis during steatohepatitis by comparing iNOS knockout (iNOS(-/-)) and wild-type (iNOS(+/+)) mice that were fed a high-fat diet. Severe fatty metamorphosis developed in the liver of iNOS(+/+) and iNOS(-/-) mice. Fibrotic changes were marked in iNOS(-/-) mice. Gelatin zymography showed that pro MMP-2 and pro MMP-9 protein expressions were more highly induced in iNOS(+/+) mice than in iNOS(-/-) mice. Active forms of MMP-2 and MMP-9 were clearly present only in the liver tissue of iNOS(+/+) mice. In situ zymography showed strong gelatinolytic activities in the liver tissue of iNOS(+/+) mice, but only spotty activity in iNOS(-/-)mice. iNOS may attenuate the progression of liver fibrosis in steatohepatitis, in part by inducing MMP-2 and MMP-9 expression and augmenting their activity.
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Base de datos:
MEDLINE
Asunto principal:
Grasas de la Dieta
/
Procolágeno
/
Óxido Nítrico Sintasa
/
Metaloproteinasas de la Matriz
/
Hígado Graso
/
Hígado
/
Cirrosis Hepática
Tipo de estudio:
Etiology_studies
Límite:
Animals
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
2005
Tipo del documento:
Article
País de afiliación:
Japón