[Esterase activity of human organotypic cornea construct (HCC) as in vitro model for permeation studies]. / Esteraseaktivität eines organotypischen humanen Kornea-Konstrukts (HCC) als In-vitro-Modell für Permeationsuntersuchungen Originalien.
Ophthalmologe
; 102(10): 971-80, 2005 Oct.
Article
en De
| MEDLINE
| ID: mdl-15785910
ABSTRACT
Organotypic cornea equivalents are used as in vitro models for permeation studies. Many ophthalmic drugs are applied as ester prodrugs to achieve a higher bioavailability. The esterase activity of three corneal human cell lines (epithelial, stromal, endothelial cells) as well as of excised porcine cornea, human donor cornea and human cornea construct (HCC) was investigated and compared. Esterase activity was determined using p-nitrophenyl acetate and hydrocortisone acetate (HCA) as esterase substrates. Hydrocortisone acetate permeation across porcine cornea, human donor cornea and HCC was studied in vitro using Franz-diffusion cells. Corneal epithelial cells showed the highest esterase activity and only small differences to keratocytes and endothelial cells were detectable. The permeation barrier properties of the different corneal tissues were very similar in the case of HCA permeation whereas HCA metabolism rates were in the ranking order of porcine cornea > HCC > human donor cornea. Permeation and metabolism studies indicate that the in vitro permeation model HCC is able to adequately convert hydrocortisone acetate to hydrocortisone.
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Base de datos:
MEDLINE
Asunto principal:
Soluciones Oftálmicas
/
Córnea
/
Esterasas
Tipo de estudio:
Prognostic_studies
Límite:
Aged
/
Animals
/
Humans
/
Middle aged
Idioma:
De
Revista:
Ophthalmologe
Asunto de la revista:
OFTALMOLOGIA
Año:
2005
Tipo del documento:
Article