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Isolation of lymphocystis disease virus from sole, Solea senegalensis Kaup, and blackspot sea bream, Pagellus bogaraveo (Brunnich).
Alonso, M C; Cano, I; Garcia-Rosado, E; Castro, D; Lamas, J; Barja, J L; Borrego, J J; Bergmann, S M.
Afiliación
  • Alonso MC; Departamento de Microbiología, Facultad de Ciencias, Universidad de Málaga, Campus Teatinos, Málaga, Spain.
J Fish Dis ; 28(4): 221-8, 2005 Apr.
Article en En | MEDLINE | ID: mdl-15813864
Two viruses were isolated from cultured sole, Solea senegalensis, and wild blackspot sea bream, Pagellus bogaraveo, and preliminarily characterized as lymphocystis disease viruses (LCDVs). Viral isolates were characterized by morphological, biochemical and biophysical properties. In addition, the susceptibility of four fish cell lines was also tested. LCDV isolates developed cytopathic effects on the SAF-1 cell line at 5 and 6 days post-infection and reached titres of 10(6) TCID50 mL(-1). The antigenic and structural protein analysis of the two new LCDV isolates showed identical profiles to that obtained for LCDV strain Leetown NFH (ATCC VR-342), used as a reference viral strain, and for an LCDV isolate collected from gilt-head sea bream, Sparus aurata, cultured in southern Spain. Molecular confirmation was performed by polymerase chain reaction. Specific primers for LCDV produced a 270-bp DNA fragment, the expected size for LCDV.
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Base de datos: MEDLINE Asunto principal: Peces Planos / Perciformes / Infecciones por Virus ADN / Enfermedades de los Peces / Iridoviridae Límite: Animals Idioma: En Revista: J Fish Dis Asunto de la revista: BIOLOGIA / MEDICINA VETERINARIA Año: 2005 Tipo del documento: Article País de afiliación: España
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Base de datos: MEDLINE Asunto principal: Peces Planos / Perciformes / Infecciones por Virus ADN / Enfermedades de los Peces / Iridoviridae Límite: Animals Idioma: En Revista: J Fish Dis Asunto de la revista: BIOLOGIA / MEDICINA VETERINARIA Año: 2005 Tipo del documento: Article País de afiliación: España