Characterization of L-arginine transport in adrenal cells: effect of ACTH.
Am J Physiol Endocrinol Metab
; 291(2): E291-7, 2006 Aug.
Article
en En
| MEDLINE
| ID: mdl-16478777
ABSTRACT
Nitric oxide synthesis depends on the availability of its precursor L-arginine, which could be regulated by the presence of a specific uptake system. In the present report, the characterization of the L-arginine transport system in mouse adrenal Y1 cells was performed. L-arginine transport was mediated by the cationic/neutral amino acid transport system y+L and the cationic amino acid transporter (CAT) y+ in Y1 cells. These Na+-independent transporters were identified by their selectivity for neutral amino acids in both the presence and absence of Na+ and by the effect of N-ethylmaleimide. Transport data correlated to expression of genes encoding for CAT-1, CAT-2, CD-98, and y+LAT-2. A similar expression profile was detected in rat adrenal zona fasciculata. In addition, cationic amino acid uptake in Y1 cells was upregulated by ACTH and/or cAMP with a concomitant increase in nitric oxide (NO) production.
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Base de datos:
MEDLINE
Asunto principal:
Arginina
/
Corteza Suprarrenal
/
AMP Cíclico
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Hormona Adrenocorticotrópica
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Sistemas de Transporte de Aminoácidos Básicos
/
Óxido Nítrico
Límite:
Animals
Idioma:
En
Revista:
Am J Physiol Endocrinol Metab
Asunto de la revista:
ENDOCRINOLOGIA
/
FISIOLOGIA
/
METABOLISMO
Año:
2006
Tipo del documento:
Article
País de afiliación:
Argentina