Preliminary evaluation of a multiplex reverse transcription-PCR assay combined with a new DNA chip hybridization assay for detecting respiratory syncytial virus.
J Clin Microbiol
; 45(11): 3811-3, 2007 Nov.
Article
en En
| MEDLINE
| ID: mdl-17881543
ABSTRACT
DNA chips represent a major advance in microbiology laboratories, enabling the detection of a wide range of possible pathogens using a single test. This study compared a multiplex reverse transcription-PCR combined with DNA chip hybridization (ProDect BCS RV chip; bcs Biotech) with the indirect immunofluorescence test commonly used to detect respiratory viruses. A total of 39 respiratory viruses (38 respiratory syncytial viruses [RSVs] and 1 influenza A virus) were detected in samples from 96 patients using the immunofluorescence test, while 36 viruses (34 RSV, 1 influenza A virus, and 1 influenza B virus) were detected by the DNA chip technique. Results showed a good level of agreement between the two tests for RSV detection; the incidence of other viruses was low, since samples were taken from patients with suspected bronchiolitis. DNA chips displayed high sensitivity (94.6%) and specificity (100%).
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Virus Sincitiales Respiratorios
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Análisis de Secuencia por Matrices de Oligonucleótidos
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
J Clin Microbiol
Año:
2007
Tipo del documento:
Article
País de afiliación:
España