[Function of F10 gene, a novel hydatidiform mole-related gene: a preliminary study].
Nan Fang Yi Ke Da Xue Xue Bao
; 28(5): 722-4, 2008 May.
Article
en Zh
| MEDLINE
| ID: mdl-18504189
ABSTRACT
OBJECTIVE:
To study the function of F10 gene, a novel hydaditiform mole-related gene.METHODS:
A549 cell line was transfected with the F10 gene of forward or reverse sequence or with the empty vector, respectively. The cellular mRNA was extracted after 24 h of transfection to screen for the differentially expressed genes among the 3 transfected and the control cells using differential display-polymerase chain reaction (ddPCR).RESULTS:
The bands representing differentially expressed genes were amplified from the cells, and the products were linked to T-Vector for sequence analysis. Several genes were screened by Blasting and their expressions were confirmed by fluorescent quantitative PCR.CONCLUSION:
F10 gene is functionally related to cell proliferation and apoptosis.
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Base de datos:
MEDLINE
Asunto principal:
Oncogenes
/
Neoplasias Uterinas
/
Mola Hidatiforme
/
Perfilación de la Expresión Génica
Límite:
Female
/
Humans
/
Pregnancy
Idioma:
Zh
Revista:
Nan Fang Yi Ke Da Xue Xue Bao
Año:
2008
Tipo del documento:
Article
País de afiliación:
China