[Function of F10 gene, a novel hydatidiform mole-related gene: a preliminary study].

ABSTRACT

OBJECTIVE: To study the function of F10 gene, a novel hydaditiform mole-related gene. METHODS: A549 cell line was transfected with the F10 gene of forward or reverse sequence or with the empty vector, respectively. The cellular mRNA was extracted after 24 h of transfection to screen for the differentially expressed genes among the 3 transfected and the control cells using differential display-polymerase chain reaction (ddPCR). RESULTS: The bands representing differentially expressed genes were amplified from the cells, and the products were linked to T-Vector for sequence analysis. Several genes were screened by Blasting and their expressions were confirmed by fluorescent quantitative PCR. CONCLUSION: F10 gene is functionally related to cell proliferation and apoptosis.