[Isolation and identification of a thermophilic bacterium from hot springs in Changbai Mountains].
Wei Sheng Wu Xue Bao
; 48(10): 1285-9, 2008 Oct.
Article
en Zh
| MEDLINE
| ID: mdl-19160805
ABSTRACT
OBJECTIVE:
We studied physiological and biochemical properties of a thermophilic bacterium isolated from hot springs in Changbai Mountain.METHODS:
The strain CBS-5 (=JCM 15484) from hot springs in Changbai Mountains was isolated by plating and screening on Olive-rich medium. It was characterized by the physiological and biochemical analysis, 16S rDNA sequencing and determination of (G+C)mol% contents.RESULTS:
The cells were Gram-positive, non-motile rods, spore-forming and generally occurred singly or in pairs. The growth temperature ranges from 48 degrees C to 70 degrees C, optimum at 65 degrees C; growth pH ranges from 6.5 to 8.5, optimum at 7.7. The strain used glucose, sucrose, maltose, lactose and rhanmose as carbon and energy sources. The G+C content of DNA was 41.9 mol%. The major cellular fatty acids were iso-C150, C160 and iso-C170, respectively representing about 24.20%, 20.45% and 17.42% of total fatty acids. The 16S rDNA sequence analysis showed that the strain belonged to the Anoxybacillus genus, for the sequence similarity was 95.1%-98.5% to other known species of Anoxybacillus.CONCLUSION:
Strain CBS-5 was a member of Anoxybacillus.
Buscar en Google
Base de datos:
MEDLINE
Asunto principal:
Bacillaceae
/
Manantiales de Aguas Termales
Tipo de estudio:
Diagnostic_studies
País/Región como asunto:
Asia
Idioma:
Zh
Revista:
Wei Sheng Wu Xue Bao
Año:
2008
Tipo del documento:
Article
País de afiliación:
China