A DNA microarray facilitates the diagnosis of Bacillus anthracis in environmental samples.
Lett Appl Microbiol
; 49(3): 324-31, 2009 Sep.
Article
en En
| MEDLINE
| ID: mdl-19552771
AIMS: In order to improve the diagnosis of Bacillus anthracis in environmental samples, we established a DNA microarray based on the ArrayTube technology of Clondiag. METHODS AND RESULTS: Total DNA of a bacterial colony is randomly biotinylated and hybridized to the array. The probes on the array target the virulence genes, the genomic marker gene rpoB, as well as the selective 16S rDNA sequence regions of B. anthracis, of the Bacillus cereus group and of Bacillus subtilis. Eight B. anthracis reference strains were tested and correctly identified. Among the analysed environmental Bacillus isolates, no virulent B. anthracis strain was detected. CONCLUSIONS: This array clearly differentiates B. anthracis from members of the B. cereus group and other Bacillus species in environmental samples by chromosomal (rpoB) and plasmid markers. Additionally, recognition of B. cereus strains harbouring the toxin genes or atypical B. anthracis strains that have lost the virulence plasmids is feasible. SIGNIFICANCE AND IMPACT OF THE STUDY: The array is applicable to the complex diagnostics for B. anthracis detection in environmental samples. Because of low costs, high security and easy handling, the microarray is applicable to routine diagnostics.
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Base de datos:
MEDLINE
Asunto principal:
Bacillus anthracis
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Técnicas Bacteriológicas
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Análisis de Secuencia por Matrices de Oligonucleótidos
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Microbiología Ambiental
Tipo de estudio:
Diagnostic_studies
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Evaluation_studies
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Prognostic_studies
Idioma:
En
Revista:
Lett Appl Microbiol
Asunto de la revista:
MICROBIOLOGIA
Año:
2009
Tipo del documento:
Article
País de afiliación:
Suiza