Separation of optical isomers in capillary chromatography using a poly(tetrafluoroethylene) capillary tube and an aqueous-organic mixture carrier solution.
Anal Sci
; 26(6): 641-3, 2010.
Article
en En
| MEDLINE
| ID: mdl-20543493
ABSTRACT
Capillary chromatography for the separation of optical isomers was developed using an untreated poly(tetrafluoroethylene) capillary tube and a water-hydrophilic/hydrophobic organic solvent mixture as a carrier solution. The open tubular capillary was 110 cm in length (90 cm effective length) and 100 microm in inner diameter. The carrier solution was prepared with a water-acetonitrile-ethyl acetate mixture (1532 volume ratio) containing 1 mM beta-cyclodextrin. A model analyte solution of dansyl-DL-methionine was injected into the capillary tube by a gravity method. The analyte solution was subsequently delivered through the capillary tube with the carrier solution by a microsyringe pump; the system worked under laminar-flow conditions. The analytes were separated through the capillary tube with on-capillary detection by an absorption detector. D-Isomer and L-isomer were eluted in this order with the water-acetonitrile-ethyl acetate carrier solution including beta-cyclodextrin.
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Base de datos:
MEDLINE
Idioma:
En
Revista:
Anal Sci
Año:
2010
Tipo del documento:
Article
País de afiliación:
Japón