An EF-hands protein, centrin-1, is an EGTA-sensitive SUMO-interacting protein in mouse testis.
Cell Biochem Funct
; 28(7): 604-12, 2010 Oct.
Article
en En
| MEDLINE
| ID: mdl-20941751
ABSTRACT
A multifunctional calcium-binding protein, centrin-1, is specifically expressed in male germ cells, certain neurons and ciliated cells. We identified centrin-1 as a protein interacting with SUMO-2/3 using yeast two-hybrid screening of a mouse testicular cDNA library. In bead halo assays, the interaction between centrin-1 and SUMO-2/3 was reduced in the presence of EGTA and facilitated by the addition of CaCl2. immunostaining of seminiferous tubules in 35-day-old mouse testes revealed that cells in the layer containing spermatogonia showed colocalization of SUMO-2/3 with centrin-1 in cytoplasmic spots. Identification of centrin-1 as the EGTA-sensitive SUMO-2/3-interacting protein indicates the possible role of calcium in modulating the centrin-1-SUMO-2/3 interaction and suggests the importance of this interaction in mouse testis.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Túbulos Seminíferos
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Espermatogonias
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Proteínas de Unión al Calcio
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Proteínas Cromosómicas no Histona
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Calcio
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Motivos EF Hand
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Proteínas Modificadoras Pequeñas Relacionadas con Ubiquitina
Tipo de estudio:
Diagnostic_studies
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Prognostic_studies
Límite:
Animals
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Humans
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Male
Idioma:
En
Revista:
Cell Biochem Funct
Año:
2010
Tipo del documento:
Article
País de afiliación:
Japón