MicroRNA signatures associated with immortalization of EBV-transformed lymphoblastoid cell lines and their clinical traits.
Cell Prolif
; 44(1): 59-66, 2011 Feb.
Article
en En
| MEDLINE
| ID: mdl-21199010
ABSTRACT
OBJECTIVE:
MicroRNAs (miRNAs) are negative regulators of gene expression that play important roles in cell processes such as proliferation, development and differentiation. Recently, it has been reported that miRNAs are related to development of carcinogenesis. The aim of this study was to identify miRNAs associated with terminal immortalization of Epstein-Barr virus (EBV)-transformed lymphoblastoid cell line (LCL) and associated clinical traits. MATERIAL ANDMETHODS:
Hence, we performed miRNA microarray approach with early- (p6) and late-passage (p161) LCLs. RESULTS ANDCONCLUSION:
Microarray data showed that nine miRNAs (miR-20b*, miR-28-5p, miR-99a, miR-125b, miR-151-3p, miR-1519.1, miR-216a, miR-223* and miR-1296) were differentially expressed in most LCLs during long-term culture. In particular, miR-125b was up-regulated in all the tested late-passage LCLs. miR-99a, miR-125b, miR-216a and miR-1296 were putative negative regulators of RASGRP3, GPR160, PRKCH and XAF1, respectively, which were found to be differentially expressed in LCLs during long-term culture in a previous study. Linear regression analysis showed that miR-200a and miR-296-3p correlated with triglyceride and HbA1C levels, respectively, suggesting that miRNA signatures of LCLs could provide information on the donor's health. In conclusion, our study suggests that expression changes of specific miRNAs may be required for terminal immortalization of LCLs. Thus, differentially expressed miRNAs would be a potential marker for completion of cell immortalization during EBV-mediated tumorigenesis.
Texto completo:
1
Base de datos:
MEDLINE
Asunto principal:
Linfocitos
/
Transformación Celular Viral
/
Herpesvirus Humano 4
/
MicroARNs
Tipo de estudio:
Prognostic_studies
/
Risk_factors_studies
Límite:
Humans
Idioma:
En
Revista:
Cell Prolif
Año:
2011
Tipo del documento:
Article
País de afiliación:
Corea del Sur